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Two distinct arginine kinases in Neocaridina denticulate: Psychrophilic and mesophilic enzymes
International Journal of Biological Macromolecules, 2014The small shrimp Neocaridina denticulata has two types of arginine kinases (AKs): AK1 and AK2. We determined the full kinetic parameters for the forward reaction of the AKs at temperatures between 15 and 35°C. The catalytic efficiencies, determined by kcat/(Ka(ATP)·Kia(ARG)), of the two enzymes showed a marked difference in temperature dependence.
Tomohiko, Suzuki, Yoshitaka, Kanou
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Temperature dependence of growth, enzyme secretion and activity of psychrophilic Antarctic bacteria
Applied Microbiology and Biotechnology, 1994Five psychrophilic Antarctic bacteria have been selected for their capacity to secrete exoenzymes into culture medium. These strains are able to grow from 0 to about 25° C. However, production of lipase fromMoraxella, α-amylase fromAlteromonas haloplanctis, β-lactamase fromPsychrobacter immobilis and protease fromBacillus is maximal at temperatures ...
Feller, Georges +5 more
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Toward a molecular understanding of cold activity of enzymes from psychrophiles
Extremophiles, 2000Despite the fact that a much greater proportion of the earth environment is cold rather than hot, much less is known about psychrophilic, cold-adapted microorganisms compared with thermophiles living at high temperatures. In particular, investigation of the molecular basis of cold-active enzymes from psychrophiles has only recently received concerted ...
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Enzymic bases of physiological changes in a mutant of the psychrophile Micrococcus cryophilus
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969Abstract The isolation and characterization of a temperature-resistant mutant of an obligate psychrophile is described. This mutant is able to grow at temperatures up to 34°, about 10° higher than the maximum growth temperature of the wild-type psychrophile.
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Molecular Adaptations of Enzymes from Thermophilic and Psychrophilic Organisms
1994Although the average temperature on earth is probably of the order of 15 °C, the environments with extreme temperature in which life can be considered possible are very common on the surface of the planet.
J. L. Arpigny +6 more
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Biotechnology Letters, 2000
The effect of different growth temperatures on the production of 11 extracellular enzymes was studied in nine cold-adapted bacterial strains isolated from various cold environments. Ten of these enzymes displayed temperature-dependent production. Five different temperature-related production patterns were identified, which depended on neither the type ...
Buchon, L. +3 more
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The effect of different growth temperatures on the production of 11 extracellular enzymes was studied in nine cold-adapted bacterial strains isolated from various cold environments. Ten of these enzymes displayed temperature-dependent production. Five different temperature-related production patterns were identified, which depended on neither the type ...
Buchon, L. +3 more
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Directed evolution study of temperature adaptation in a psychrophilic enzyme.
Journal of molecular biology, 2000We have used laboratory evolution methods to enhance the thermostability and activity of the psychrophilic protease subtilisin S41, with the goal of investigating the mechanisms by which this enzyme can adapt to different selection pressures. A combined strategy of random mutagenesis, saturation mutagenesis and in vitro recombination (DNA shuffling ...
Miyazaki, Kentaro +4 more
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Aspartate aminotransferase from Moraxella TAC125: an unusual psychrophilic enzyme
1999Life at low temperature requires molecular modifications that are not just the opposite to those adopted by thermophilic organisms. Hot-adapted proteins, indeed, have to face the problem of stability at high temperature, whereas the psychrophilic ones have to cope with the reduction of the enzymatic reaction rates at low temperature in order to ...
M. L. Tutino +6 more
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About the pecularities of psychrophilic enzymes
Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 2000Ch. Gerday, G. Feller
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