Results 231 to 240 of about 50,195 (270)

Immunohistochemical investigation of pulmonary surfactant-associated protein A in fire victims

Legal Medicine, 2001
To evaluate the forensic pathological significance of the immunohistochemical distribution of pulmonary surfactant-associated protein A (SP-A) in determining the cause of death in fires, 57 fire victims were examined by scoring the staining intensity.
B L, Zhu, K, Ishida, S, Oritani, L, Quan, M, Taniguchi, D R, Li, M Q, Fujita, H, Maeda   +7 more
openaire   +2 more sources

Characterization of a Second Human Pulmonary Surfactant-associated Protein SP-A Gene

American Journal of Respiratory Cell and Molecular Biology, 1992
Pulmonary surfactant is a lipid-protein complex involved in maintaining alveolar stability. SP-A is the major surfactant-associated protein of 26 to 38 kD. A human SP-A gene (SP-A I) and two distinct SP-A cDNAs, MPSAP 1A and MPSAP 6A, have been reported previously.
S L, Katyal, G, Singh, J, Locker
openaire   +2 more sources

Immunohistochemistry of pulmonary surfactant-associated protein A in acute respiratory distress syndrome

Legal Medicine, 2001
Acute respiratory distress syndrome (ARDS) is a fatal complication in severe traumas and diseases. Although the contribution of pulmonary surfactant abnormality to the pathogenesis of ARDS has been clinically fairly well investigated, the histopathological evidence has not been established.
B L, Zhu, K, Ishida, L, Quan, M Q, Fujita, H, Maeda   +4 more
openaire   +2 more sources

Proline hydroxylation alters the electrophoretic mobility of pulmonary surfactant‐associated protein A

ELECTROPHORESIS, 1988
AbstractStudies from several laboratories involving amino acid analysis and sequencing of the Mr 35 000 pulmonary surfactant‐associated proteins (SP‐A) have detected hydroxyproline residues. These residues are present in a region with a collagen‐like sequence that has been revealed by direct amino acid sequencing and from the deduced amino acid ...
D S, Phelps, J, Floros
openaire   +2 more sources

A Quantitative Method for Detecting Surfactant-Associated Protein C in Pulmonary Surfactant

Analytical Biochemistry, 1994
We describe a chemical method for the detection and quantification of surfactant protein C (SP-C), a palmitoylated 3.5-kDa protein, based on the release of free-sulfhydryl groups upon depalmitoylation. SP-C was purified from calf lung surfactant extract by gel filtration on LH-20 and LH-60 columns in chloroform:methanol:0.1 N HCl (19:19:2).
R, Qanbar, F, Possmayer
openaire   +2 more sources

Isolation and sequence of a cDNA clone for the rat pulmonary surfactant-associated protein (PSP-A)

Biochemical and Biophysical Research Communications, 1987
Pulmonary surfactant is composed mainly of phospholipid and two groups of apoproteins. One of these apoproteins is a family of glycoproteins (pulmonary surfactant-associated protein A, PSP-A). We have isolated and sequenced a cDNA clone encoding for rat PSP-A and the full amino acid sequence has been deduced from the nucleotide sequence.
K, Sano, J, Fisher, R J, Mason, Y, Kuroki, J, Schilling, B, Benson, D, Voelker   +6 more
openaire   +2 more sources

Determination of the Pulmonary Surfactant-Associated Protein SP-B in Amniotic Fluid with a Competition ELISA

Gynecologic and Obstetric Investigation, 1994
A competition ELISA was developed with polyclonal chicken antibodies, with which the surfactant-associated protein B (SP-B) can be measured in amniotic fluid. The SP-B content from 240 amniotic fluid samples taken from uncomplicated pregnancies between the 29th and 42nd week of gestation was determined.
I, Dilger, G, Schwedler, J W, Dudenhausen   +2 more
openaire   +2 more sources

Pulmonary surfactant-associated protein A levels in cadaveric sera with reference to the cause of death

Forensic Science International, 2000
Pulmonary surfactant-associated protein A (SP-A) is an exclusively lung specific protein, and is considered to leak into the blood stream in alveolar septal damage. In this study we examined the serum SP-A level in forensic autopsy materials using an enzyme immunoassay with monoclonal antibodies to assess the postmortem level in relation to the cause ...
K, Ishida, B, Zhu, L, Quan, M Q, Fujita, H, Maeda   +4 more
openaire   +2 more sources

Development of the pulmonary acinus in fetal rat lung: a study based on an antiserum recognizing surfactant-associated proteins

Anatomy and Embryology, 1987
In this study on the development of the pulmonary acinus in fetal rat lung use was made of an antiserum, rabbit anti-mouse, that recognizes the type II alveolar epithelial cell or its precursor (a cuboidal cell lacking multilamellar bodies) by the presence of a cell-specific antigen. This serum had already been used in studies on mouse-lung development
C J, Otto-Verberne, A A, Ten Have-Opbroek   +1 more
openaire   +2 more sources

Pulmonary surfactant-associated protein A enhances the surface activity of lipid extract surfactant and reverses inhibition by blood proteins in vitro

Biochemistry, 1990
Although a monolayer of dipalmitoylphosphatidylcholine, the major component of pulmonary surfactant, is thought to be responsible for the reduction of the surface tension at the air-liquid interface of the alveolus, the participation of unsaturated and anionic phospholipids and the three surfactant-associated proteins is suggested in the generation and
A M, Cockshutt, J, Weitz, F, Possmayer
openaire   +2 more sources