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FISH of Alu-PCR amplified YAC clones and applications in tumor cytogenetics [PDF]

, 1994
Choo, K. H. A., Cremer, Thomas, Lengauer, Christoph, Speicher, Michael R.   +3 more
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Pulsed Field Gel Electrophoresis

2003
Pulsed- field gel electrophoresis (PFGE) was first described by Schwartz and Cantor (1) It is now an umbrella term for the alternating of an electric field in more than one direction through a solid matrix to achieve the separation of DNA fragments.
A J, Hillier, B E, Davidson
  +8 more sources

Pulsed-field gel electrophoresis

Nature Protocols, 2007
This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel electrophoresis commonly resolves fragments up to approximately 50 kb in size, PFGE fractionates DNA molecules up to 10 Mb.
Jill, Herschleb, Gene, Ananiev, David C, Schwartz   +2 more
openaire   +3 more sources

Pulsed field sequencing gel electrophoresis

ELECTROPHORESIS, 1992
AbstractThe effect of pulsed fields on sequencing gel electrophoresis is investigated, using DNA fragment markers ranging in size from 20 to 6557 bases. For high continuous electric fields (5000 V/55 cm) band inversion is observed in which fragments larger than 4000 bases migrate faster than those of 800–1000 bases.
E, Brassard, C, Turmel, J, Noolandi
openaire   +2 more sources

Pulsed field gel electrophoresis

Analytical Chemistry, 1991
The development of pulsed field gel electrophoresis has increased by 2 orders of magnitude the size of DNA molecules that can be routinely fractionated and analyzed. This increase is of major importance to molecular biology because it simplifies many previously laborious investigations and makes possible many new ones.
openaire   +4 more sources

Pulsed-field gel electrophoresis

Molecular Biotechnology, 1998
Pulsed-field gel electrophoresis (PFGE) was originally developed as a technique for providing electrophoretic karyotypes of micro-organisms. Since then the technique has evolved and diversified in many new directions. This review traces the evolution of PFGE, summarizes our understanding of its theoretical basis, and provides a comprehensive ...
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Pulsed-field gel electrophoresis

1992
In view of the great impact of restriction fragment length polymorphism (RFLP) markers on both basic and applied plant research, considerable effort is currently being spent in constructing complete genetic linkage maps of the genomes of a wide variety of plant species.
Raymond Van Daelen, Pim Zabel
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Pulsed-field gel electrophoresis.

Methods in enzymology, 1996
Campylobacter is a common foodborne pathogen. In most outbreaks, the source of infection is not found, due to the time passing from infection to diagnosis. In processed foods, Campylobacter may also become stressed and difficult to culture. In milkborne outbreaks, the contaminated milk is usually not kept long enough, and the bulk milk is not ...
J C, Wrestler, B D, Lipes, B W, Birren, E, Lai   +3 more
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Pulsed‐Field Gel Electrophoresis

Current Protocols in Molecular Biology, 2000
AbstractDNA molecules longer than 25 kb are poorly resolved by standard agarose gel electrophoresis. These longer molecules can be resolved using several techniques that periodically change the direction of the electric field in the gel. This unit describes the simplest and most generally useful of the pulsed‐field techniques, field inversion ...
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Pulse Field Gel Electrophoresis

2015
International ...
Jordan, Kieran, Dalmasso, Marion
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