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Pulsed-Field Gel Electrophoresis
Genome, 1989The introduction of pulsed — field — gel techniques for separating large DNA molecules has had an invigorating effect on the study of chromosomal DNA molecules, genome structure and electrophoretic theory. Indeed, the story of pulsed — field gels--like those of the Southern experiment or the polymerase-chain reaction--is a good illustration of the ...
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Methods for pulsed-field gel electrophoresis
Applied Biochemistry and Biotechnology, 1993The term pulsed-field gel electrophoresis (PFGE) is used as an acronym to indicate any technique that resolves (large) DNA molecules by continuous reorientation. It bridges the resolution gap between cytogenetic methods (> 5 Mb) and DNA analysis (< 50 kb).
J T, Den Dunnen, G J, van Ommen
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Pulsed Field Gel Electrophoresis for Bifidobacterium
2015Pulse Field Gel Electrophoresis (PFGE), unlike conventional electrophoresis, can resolve DNA fragments greater than 30 kb and is a highly discriminatory molecular typing method. Here we describe a PFGE protocol for bifidobacteria characterized by a short lysis time determined by the addition of lysis reagents to the initial cell suspension, a reduced ...
Esther, Jiménez +2 more
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Pulsed Field Gel Electrophoresis for Mycobacteria
2003Reference EPFL-CHAPTER-151283doi:10.1385/0-89603-471-2:51View record in PubMed Record created on 2010-09-07, modified on 2017-05 ...
W J, Philipp +3 more
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Pulsed Field Gel Electrophoresis
1995Abstract Pulsed field gel electrophoresis (PFGE) is the most reliable method for separating large fragments of DNA and has had a significant impact on the analysis of both prokaryotic and eukaryotic genomes. This book presents detailed chapters on both the theory behind PFGE and protocols for most of its major research and diagnostic ...
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Pulsed-Field Gel Electrophoresis of MRSA
2007Pulsed-field gel electrophoresis (PFGE) is a genetic typing method that is widely used as a molecular epidemiological tool for studying the genetic diversity of Staphylococcus aureus and numerous other bacterial pathogens. For PFGE, intact bacterial cells are embedded in soft agarose plugs followed by lysis of the cell wall in situ to minimize shearing
Kurt D, Reed +2 more
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Pulsed-field Gel Electrophoresis via Transverse Alternating Field Electrophoresis Gels
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Theories of Pulsed-Field Gel Electrophoresis
2003Pulsed-field gel electrophoresis (PFGE) is one of the key technological advances of the past ten years that has made the mapping of genomes of whole organisms possible. In conventional electrophoreis, the mobility of DNA at almost any practical value of the field strength is essentially independent of mol wt above approx 30 kbp.
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Pulsed-Field Gel Electrophoresis: Protocols
1999Pulsed-field gel electrophoresis (PFGE) techniques in combination with the cloning of large fragments of DNA into yeast artificial chromosomes (YACs) have revolutionized physical mapping in molecular genetics (Barlow and Lehrach 1987; Burke et al. 1987).
Gudrun A. Rappold +4 more
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