Results 211 to 220 of about 20,168 (266)

LINGCOD MUSCLE PURINE NUCLEOSIDE PHOSPHORYLASE

Canadian Journal of Biochemistry and Physiology, 1958
A purine nucleoside phosphorylase enzyme preparation, which catalyzed the general reaction ribose (deoxyribose) [Formula: see text] nucleoside (deoxynucleoside)+orthophosphate, was isolated from muscles of the lingcod (Ophiodon elongatus). The reaction was found to be about 85% in favor of nucleoside synthesis with 10 μM./ml.
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Prenatal exclusion of purine nucleoside phosphorylase deficiency

European Journal of Pediatrics, 1986
We report on the prenatal exclusion of purine nucleoside phosphorylase (PNP) deficiency in a fetus whose parents were known to be heterozygotes for the enzyme defect. Prenatal investigation was performed in the 16th week of gestation on amniotic fluid and cultured amnion cells using sensitive techniques.
E. Carapella De Luca, STEGAGNO, MICHELE, C. Dionisi Vici, PAESANO, Rosalba, L. D. Fairbanks, G. S. Morris, H. A. Simmonds   +6 more
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Purine nucleoside phosphorylase: Isolation and characterization

Collection of Czechoslovak Chemical Communications, 1990
Purine nucleoside phosphorylase (PNP) from mouse leukemia cells L1210 was purified to homogeneity by a combination of ion exchange and affinity chromatography using AE-Sepharose 4B and 9-(p-succinylaminobenzyl)hypoxanthine as the matrix and the ligand, respectively.
Antonín Holý, Katarina Šedivá, Ivan Rosenberg, Ivan Votruba   +3 more
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Is purine nucleoside phosphorylase an example of a morpheein?

Journal of Physics: Condensed Matter, 2007
Purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme of the nucleoside salvage pathway and it is characterized by non-Michaelis kinetics. Kinetic data in many cases (for some substrates or some concentration range of a co-substrate) are best described by the double hyperbolic equation.
Borys Kierdaszuk, Anna Modrak-Wójcik, Katarzyna Stępniak, Agnieszka Bzowska   +3 more
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Design of Purine Nucleoside Phosphorylase Inhibitors

2018
Purine nucleoside phosphorylase inhibitors hold promise as specific immunosuppressive, anti-T cell leukemic, and antiuricopoietic agents. The best inhibitors available that are biologically active have Ki values from 10(-6) to 10(-7) M and fall into two categories: noncleavable nucleosides preferably iodinated at the C-5' position and C-8-substituted ...
Y. Sudhakara Babu, John A. Montgomery, Charles E. Bugg, W. Michael Carson, Sthanam V. L. Narayana, William J. Cook, Steven E. Ealick, Wayne C. Guida, Mark D. Erion, John A. Secrist   +9 more
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Purine nucleoside phosphorylase from bovine liver

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1978
1. Purine nucleoside phosphorylase (purine nucleoside:orthophosphate ribosyltransferase, E.C. 2.4.2.1) from liver of cattle, Bos taurus, was purified to homogeneity. Some properties of the enzymes from three different bovine tissues were compared and discussed. 2.
Takeshi Nishino, Zenro Ikezawa, Koji Murakami, Keizo Tsushima   +3 more
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Purine Nucleoside Phosphorylase. 2. Catalytic Mechanism

Biochemistry, 1997
X-ray crystallography, molecular modeling, and site-directed mutagenesis were used to delineate the catalytic mechanism of purine nucleoside phosphorylase (PNP). PNP catalyzes the reversible phosphorolysis of purine nucleosides to the corresponding purine base and ribose 1-phosphate using a substrate-assisted catalytic mechanism.
Richard L. Walter, Steven E. Ealick, Mark D. Erion, Johanna D. Stoeckler, Wayne C. Guida   +4 more
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16 Purine Nucleoside Phosphorylase

1972
Publisher Summary This chapter focuses on purine nucleoside phosphorylases (PNPases), which are widely distributed in nature and have been identified and studied in a number of tissues in mammals, chickens, fish, yeast, and in several species of bacteria. In the erythrocytes of dogs, the activity of PNPase is remarkably low when compared with the red
R.E. Parks, R.P. Agarwal
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A new colorimetric assay for purine nucleoside phosphorylase

Clinical Biochemistry, 1989
A new colorimetric procedure for the determination of purine nucleoside phosphorylase (PNP, E.C. 2.4.2.1) activity is described. In this procedure, the hydrogen peroxide formed in the PNP-xanthine oxidase reaction is used to oxidize the chromogenic reagents--3,5-dichloro-2-hydroxybenzenesulfonic acid/4-aminophenazone using the enzyme peroxidase.
Peter Cashion, Samuel Y. Chu, Min Jiang
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A new ultramicrochemical assay for purine nucleoside phosphorylase

Analytical Biochemistry, 1978
Abstract An ultramicrochemical method using radioactive substrates has been developed for the quantitation of purine nucleoside phosphorylase (NP) activity in single cells. NP activity can reproducibly be measured in both directions of the enzyme reaction. A quantitative relationship between the number of cells incubated and the NP activity was found.
C. H. M. M. de Bruyn, P. Hösli, T. L. Oei, Claude Griscelli, M.P. Uitendaal   +4 more
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