Results 221 to 230 of about 24,465 (266)
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Purine nucleoside phosphorylase of chicken liver
Biochimica et Biophysica Acta (BBA) - Enzymology, 1971Abstract Purine nucleoside phosphorylase (purine nucleoside:orthophosphate ribosyltransferase, EC 2.4.2.1) has been purified 125-fold from the homogenate of chicken livers and some of the properties of the purified enzyme have been studied. This enzyme had a pH optimum at around 6.o. At high substrate levels of inosine the reaction rate was increased,
Keizo Tsushima +2 more
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Characterization of purine nucleoside phosphorylase in leukemia
American Journal of Hematology, 1986AbstractPurine nucleoside phosphorylase (PNP) activity was determined in mononuclear cells from 49 patients with various types of leukemia. A low level of PNP activity was found in mononuclear cells from patients with acute myeloid and lymphoblastic leukemia and with chronic lymphocytic leukemia. Enzymatic and immunological studies on PNP from leukemic
Naomi Horiuchi +3 more
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Adenine as Substrate for Purine Nucleoside Phosphorylase
Canadian Journal of Biochemistry, 1971Purine nucleoside phosphorylase (purine-nucleoside:orthophosphate ribosyltransferase, EC 2.4.2.1) from four sources (rat liver and brain, human erythrocytes, and calf spleen) has been shown to exhibit a low intrinsic activity towards adenine in the presence of ribose 1-phosphate.
R. P. Miech +3 more
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Allosteric regulation of purine nucleoside phosphorylase
Archives of Biochemistry and Biophysics, 1991Purine nucleoside phosphorylase (EC 2.4.2.1) from bovine spleen is allosterically regulated. With the substrate inosine the enzyme displayed complex kinetics: positive cooperativity vs inosine when this substrate was close to physiological concentrations, negative cooperativity at inosine concentrations greater than 60 microM, and substrate inhibition ...
Patricia A. Ropp, Thomas W. Traut
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Stroke in purine nucleoside phosphorylase deficiency
Pediatric Neurology, 1995The first documented case of cerebrovascular disease occurring in a 13-year-old girl with purine nucleoside phosphorylase deficiency is reported. This patient, the oldest known survivor with purine nucleoside phosphorylase deficiency, had previously experienced multiple sequential neurologic problems.
David A. Tam, Robert T. Leshner
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LINGCOD MUSCLE PURINE NUCLEOSIDE PHOSPHORYLASE
Canadian Journal of Biochemistry and Physiology, 1958A purine nucleoside phosphorylase enzyme preparation, which catalyzed the general reaction ribose (deoxyribose) [Formula: see text] nucleoside (deoxynucleoside)+orthophosphate, was isolated from muscles of the lingcod (Ophiodon elongatus). The reaction was found to be about 85% in favor of nucleoside synthesis with 10 μM./ml.
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Prenatal exclusion of purine nucleoside phosphorylase deficiency
European Journal of Pediatrics, 1986We report on the prenatal exclusion of purine nucleoside phosphorylase (PNP) deficiency in a fetus whose parents were known to be heterozygotes for the enzyme defect. Prenatal investigation was performed in the 16th week of gestation on amniotic fluid and cultured amnion cells using sensitive techniques.
E. Carapella De Luca +6 more
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Design of Purine Nucleoside Phosphorylase Inhibitors
2018Purine nucleoside phosphorylase inhibitors hold promise as specific immunosuppressive, anti-T cell leukemic, and antiuricopoietic agents. The best inhibitors available that are biologically active have Ki values from 10(-6) to 10(-7) M and fall into two categories: noncleavable nucleosides preferably iodinated at the C-5' position and C-8-substituted ...
Y. Sudhakara Babu +9 more
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Purine nucleoside phosphorylase: Isolation and characterization
Collection of Czechoslovak Chemical Communications, 1990Purine nucleoside phosphorylase (PNP) from mouse leukemia cells L1210 was purified to homogeneity by a combination of ion exchange and affinity chromatography using AE-Sepharose 4B and 9-(p-succinylaminobenzyl)hypoxanthine as the matrix and the ligand, respectively.
Antonín Holý +3 more
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Is purine nucleoside phosphorylase an example of a morpheein?
Journal of Physics: Condensed Matter, 2007Purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme of the nucleoside salvage pathway and it is characterized by non-Michaelis kinetics. Kinetic data in many cases (for some substrates or some concentration range of a co-substrate) are best described by the double hyperbolic equation.
Borys Kierdaszuk +3 more
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