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A new isotopic assay for purine nucleoside phosphorylase
Analytical Biochemistry, 1989We have developed a new assay for purine nucleoside phosphorylase which is based on the release of tritium when [2-3H]inosine is used as the substrate and the reaction is coupled with xanthine oxidase. After the reaction is terminated, residual [2-3H]inosine is adsorbed on charcoal and the supernatant solution is assayed for radioactivity by liquid ...
Chi-Hsiung Chang +2 more
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Inhibitors of the enzyme purine nucleoside phosphorylase
Expert Opinion on Therapeutic Patents, 1998The enzyme purine nucleoside phosphorylase (PNP) catalyses the reversible cleavage of purine nucleosides to the purine base and ribose-1-phosphate. Several cases of a rare genetic disorder in which PNP is lacking have been reported in children. These children were found to be T-cell immunodeficient while their B-cell immunity remained normal.
Philip E. Morris, John A. Montgomery
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Purine nucleoside phosphorylase polymorphism in sheep erythrocytes
Biochemical Genetics, 1977A polymorphism of purine nucleoside phosphorylase is described in sheep erythrocytes. Two isozymes were distinguished electrophoretically, one with high activity (NP-1) and one with low activity (NP-2). Breeding data suggest that the two isozymes are the product of two codominant alleles, NP1 and NP2.
P. G. Board, J. E. Smith
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Purine Nucleoside Phosphorylase Deficiency: A Mutation Update
Nucleosides, Nucleotides and Nucleic Acids, 2011Purine nucleoside phosphorylase (PNPase) deficiency is an autosomal recessive disorder affecting purine degradation and salvage pathways. Clinically, patients typically present with severe immunodeficiency, neurological dysfunction, and autoimmunity. Biochemically, PNPase deficiency may be suspected in the presence of hypouricemia.
A. Corrigan +5 more
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Genetic Variability of Purine Nucleoside Phosphorylase in the Mouse
1984The inherited human deficiency of purine nucleoside phosphorylase is associated primarily with cellular immune dysfunction1. We have begun to screen for quantitative activity variants of purine nucleoside phosphorylase in Mus musculus in attempts to establish an animal model for the enzyme deficiencies associated with immunodeficiency disease.
Marcia J. Sparling +3 more
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Chapter 3. Purine Nucleoside Phosphorylase
2007Purine nucleoside phosphorylase (PNP) catalyzes the reversible phosphorolysis of purine ribonucleosides and 2′-deoxypurine ribonucleosides to the free base, and ribose 1-phosphate or 2′-deoxyribose 1-phosphate, respectively.1–3 PNPs are found in most prokaryotic and eukaryotic organisms where the en...
Steven E. Ealick, Yang Zhang
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Plasma purine nucleoside phosphorylase in cancer patients
Clinica Chimica Acta, 2004Purine nucleoside phosphorylase (PNP) is the purine salvage enzyme that converts guanosine to guanine and inosine to hypoxanthine.279 samples from patients with differing cancers were collected during treatment at both pre- and post-dose stages for plasma PNP activity and compared with a normal population.Normal plasma PNP activity was found to be 3.2+/
Russell P. Newton +2 more
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Functional analysis of purine nucleoside phosphorylase as a key enzyme in ribavirin metabolism.
Drug Metabolism and Pharmacokinetics, 2014Ribavirin is a purine nucleoside analogue that possesses potent anti-hepatitis C virus activity, and it has long been considered likely that ribavirin undergoes a first-pass metabolism at the small intestine. Although purine nucleoside phosphorylase (PNP)
Tomomi Furihata +5 more
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[73] Chinese hamster purine nucleoside phosphorylase
1978Publisher Summary This chapter describes the purification procedure of nucleoside phosphorylase enzyme from Chinese hamster V79 tissue culture cells. Purine nucleoside phosphorylase (purine nucleoside: orthophosphate ribosyltransferase) in eukaryotes catalyzes the reversible conversion among the purine bases, hypoxanthine and guanine, and their ...
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