Results 71 to 80 of about 27,209 (247)
Synthesis and enzymatic evaluation of the guanosine analogue 2-amino-6-mercapto-7-methylpurine ribonucleoside (MESG): insights into the phosphorolysis reaction mechanism based on the blueprint transition state: SN1 or SN2? [PDF]
, 2010 A modified experimental procedure for the synthesis of MESG (2-amino-6-mercapto-7-methylpurine ribonucleoside) 1 has been successfully performed and its full characterization is presented.
Basso, Luiz A. +8 more
core +2 more sources
Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases. [PDF]
PLoS ONE, 2012 The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies.
Priscila O de Giuseppe +5 more
doaj +1 more source
, 2014 The standard of care for patients suffering cancer often includes treatment with nucleoside analogues (NAs). NAs are internalized by cell-specific nucleobase/nucleoside transporters and, after enzymatic activation (often one or more phosphorylation steps)
Balzarini, Jan +2 more
core
Pinpointing dynamic coupling in enzymes for efficient drug design [PDF]
, 2016 Enzymes are proteins that catalyze almost every chemical reaction in living systems, achieving rate enhancements of up to 21 orders of magnitude relative to the uncatalyzed reactions.
Glasstone S, Haldane JBS, Polanyi M
core +1 more source
Pharmacoepidemiology and Drug Safety, Volume 35, Issue 4, April 2026.ABSTRACT Immunocompromised individuals experience an impaired immune function due to conditions that might be either congenital or acquired over the course of their lives. Epidemiological studies often rely on clinical definitions which, in some cases, benefit from being translated into machine‐readable algorithms for application to electronic health ...
Judit Riera‐Arnau +62 more
wiley +1 more source
A Simple Method for RNA‐Seq of Manually Isolated Chromatophores in Oryzias Fishes
Development, Growth &Differentiation, Volume 68, Issue 3, April 2026.ABSTRACT RNA sequencing (RNA‐seq) has become an essential tool for analyzing gene expression and exploring cell type–specific transcriptomes. However, sample preparation and quality control remain challenging, as current approaches typically rely on dissecting tissues containing mixed cell populations or using flow cytometry to isolate fluorescently ...
Makoto Goda +11 more
wiley +1 more source
The cytokinesis regulator RacGAP1 is a Rac1‐specific GAP on membranes
Protein Science, Volume 35, Issue 3, March 2026.Abstract Rho family small GTPases are essential for cytokinesis completion. RacGAP1, a dimeric multidomain protein with a lipid‐binding C1 domain and a GTPase‐activating protein (GAP) domain, is a major regulator in this process. However, despite many cellular and biochemical studies, whether RhoA or Rac1 is the actual substrate inactivated by RacGAP1 ...
Pavlina Dubois +5 more
wiley +1 more source
Molecules, 2019 Etheno-derivatives of guanine, O6-methylguanine, and isoguanine were prepared and purified using standard methods. The title compounds were examined as potential substrates of purine-nucleoside phosphorylases from various sources in the reverse ...
Alicja Stachelska-Wierzchowska +4 more
doaj +1 more source
Proteomic alterations of Escherichia coli by paraquat [PDF]
, 2010 Paraquat (PQ; a widely used herbicide) exerts its harmful effect to human, mammals and microorganisms upon intracellular conversion to superoxide radical.
Isarankura-Na-Ayudhya, Chartchalerm +7 more
core
Global transcriptome response in during growth on ribose [PDF]
, 2011 Background Lactobacillus sakei is valuable in the fermentation of meat products and exhibits properties that allow for better preservation of meat and fish. On these substrates, glucose and ribose are the main carbon sources available for growth. We used
Naterstad Kristine +3 more
core +2 more sources