Results 111 to 120 of about 159,015 (286)
Advanced Science, EarlyView.This study identifies B4GALNT4 as a key driver of prostate cancer (PCa). It shows that B4GALNT4 glycosylates PDK1 protein at the N531 site, which stabilizes the PDK1 and constitutively activates the PI3K‐AKT pathway. This mechanism promotes tumor cell proliferation, migration, and invasion. The findings establish the B4GALNT4‐PDK1 glycosylation axis as
Shaoqin Jiang +12 more
wiley +1 more source
An Enhanced Retroviral Vector for Efficient Genetic Manipulation and Selection in Mammalian Cells
BiomoleculesIntroducing genetic material into hard-to-transfect mammalian cell lines and primary cells is often best achieved through retroviral infection. An ideal retroviral vector should offer a compact, selectable, and screenable marker while maximizing ...
Jana Triller +3 more
doaj +1 more source
Full-length prepro-alpha-factor can be translocated across the mammalian microsomal membrane only if translation has not terminated. [PDF]
, 1988 We have previously shown that fully synthesized prepro-alpha-factor (pp alpha F), the precursor for the yeast pheromone alpha-factor, can be translocated posttranslationally across yeast rough microsomal (RM) membranes from a soluble, ribosome-free pool.
Garcia, PD, Walter, P
core
Advanced Science, EarlyView.CDK4/6 inhibition promotes CD8+ T cell expansion through tumor‐macrophage crosstalk by activating HIF‐1α and enhancing MIF‐CD44/CD74 signaling. This reprograms TAMs to boost MHC‐I antigen presentation, and CDK4/6 inhibitor‐trained M1 TAM supernatant therapy synergizes with low‐dose PD‐1 blockade to restore antitumor immunity.
Lin He +17 more
wiley +1 more source
Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines
Bio-Protocol, 2018 The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV.
Jin-Wei Zheng, Jia-Li Cao, Quan Yuan
doaj +1 more source
Advanced Science, EarlyView.Chronic myeloid leukemia blast phase (CML‐BP) poses a severe therapeutic challenge. This study reveals that the super‐enhancer‐driven transcription factors SOX4 and SMAD3 form a cooperative axis critical for disease progression. They co‐activate the oncogenic kinase AXL and promote phospholipid remodeling via LPCAT1 to facilitate its signaling ...
Enzhe Lou +22 more
wiley +1 more source
lHuman cytotoxic T lymphocytes with reduced sensitivity to Fas-induced apoptosis [PDF]
, 2005 Effector-memory T cells expressing Fas (Apo-1/CD95) are switched to an apoptotic program by cross-linking with Fas-ligand (FasL). Consequently, tumors that express FasL can induce apoptosis of infiltrating Fas-positive T lymphocytes and subdue any ...
Biagi, E +8 more
core
A Bayesian measurement error model for two-channel cell-based RNAi data with replicates
, 2012 RNA interference (RNAi) is an endogenous cellular process in which small double-stranded RNAs lead to the destruction of mRNAs with complementary nucleoside sequence. With the production of RNAi libraries, large-scale RNAi screening in human cells can be
Chang, I-Shou +8 more
core +1 more source
ITGB1 Regulates Triple‐Negative Breast Cancer Development by Modulating the Tumor Microenvironment
Advanced Science, EarlyView.ABSTRACT Tumorigenesis and metastasis are frequently attributed to the intricate interplay between cancer cells and the tumor microenvironment (TME). Comprehending the mechanisms and key regulators of cancer‐immune crosstalk in the TME is imperative for developing efficacious immunotherapy.
Nuozi Song +12 more
wiley +1 more source
Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses
Molecular Therapy: Methods & Clinical Development, 2019 We describe a strategy to achieve footprintless bi-allelic homology-directed repair (HDR) using helper-dependent adenoviruses (HDAds). This approach utilizes two HDAds to deliver the donor DNA.
Donna J. Palmer +2 more
doaj +1 more source