Results 101 to 110 of about 126,114 (304)

Genome‐Wide CRISPR Screen Identifies a microRNA Orchestrating Pleiotropic Resistance to Targeted Therapy and T Cell Immunity in Melanoma

Advanced Science, EarlyView.
A genome‐wide microRNA CRISPR screen identifies miR‐18a as a master regulator of cross‐resistance in melanoma. Loss of miR‐18a activates the AJUBA–YAP/Hippo axis to confer BRAFi resistance and enhances THBS1–CD47 interaction to impair CD8+ T cell immunity. hnRNP A1 is identified as an upstream regulator of miR‐18a processing.
Zhao Wang, Haotian Liu, Xueqi Wang, Jianjin Teng, Zihan Zheng, Jingya Zhang, Wanyi Xiao, Qian Liang, Jiaxin Li, Xiaomeng Jia, Xin Feng, Hongzhang Cui, Menghan Luo, Tielong Yang, Liuxing Wu, Ke Zhao, Weilong Yang, Mulin Jun Li, Dandan Huang, Jilong Yang   +19 more
wiley   +1 more source

A novel puromycin decorporation method to quantify skeletal muscle protein breakdown: a proof-of-concept study [PDF]

, 2017
The precise roles that the major proteolytic pathways play in the regulation of skeletal muscle mass remain incompletely understood, in part due to technical limitations associated with current techniques used to quantify muscle protein breakdown (MPB ...
Wilkinson, Daniel J., Smith, Kenneth, Atherton, Philip J., Hannah Crossland, Philip J. Atherton, Daniel J. Wilkinson, Kenneth Smith, Crossland, Hannah   +7 more
core   +1 more source

Scarless Genome Editing of Human Pluripotent Stem Cells via Transient Puromycin Selection

Stem Cell Reports, 2018
Summary: Genome-edited human pluripotent stem cells (hPSCs) have broad applications in disease modeling, drug discovery, and regenerative medicine. We present and characterize a robust method for rapid, scarless introduction or correction of disease ...
Benjamin Steyer, Qian Bu, Evan Cory, Keer Jiang, Stella Duong, Divya Sinha, Stephanie Steltzer, David Gamm, Qiang Chang, Krishanu Saha   +9 more
doaj   +1 more source

RNA Helicase DDX21 Controls CD4+ T Cell Proliferation and Promotes Inflammatory Bowel Disease via Translational Control

Advanced Science, EarlyView.
ABSTRACT Inflammatory bowel disease (IBD) is characterized by dysregulated T cell responses. RNA helicases, including DExD‐box helicase 21 (DDX21), are pivotal in RNA metabolism, but their role in T cell‐mediated pathology during IBD remains unclear. Here, we demonstrate that DDX21 expression in CD4+ T cells correlates with cell cycle and translation ...
Yujuan Zhang, Chen Kan, Xinhui Yang, Yajuan Hao, Xuemin Cai, Mei Yang, Qianqian Zhang, Zhengting Wang, Ning Jiang, Lei Wang, Hua‐Bing Li, Jing Zhou   +11 more
wiley   +1 more source

Potential energy calculations on conformations of puromycin and 3'-terminal aminoacyl adenosines of transfer RNAs

, 1973
Potential energy calculations taking into account van der Waals, electrostatic and torsional interactions have been carried out on puromycin, an inhibitor of protein synthesis, resembling the terminal 3'-aminoacyl adenosine moieties of charged transfer ...
Sundaralingam, M., Yathindra, N.
core   +1 more source

Electron microscopic and biochemical study of lipoprotein synthesis in the isolated perfused rat liver

Journal of Lipid Research, 1967
The isolated perfused rat liver was used to study the 300-800 A electron-opaque bodies which had previously been described in the liver cell Golgi apparatus, smooth endoplasmic reticulum, and space of Disse.When the perfusion medium was enriched with ...
Albert L. Jones, Neil B. Ruderman, M. Guillermo Herrera   +2 more
doaj   +1 more source

Isolation and properties of a puromycin acetyltransferase from puromycin-producing Streptomyces alboniger.

The Journal of Antibiotics, 1985
Puromycin 2"-N-acetyltransferase was isolated from cell extracts of puromycin-producing Streptomyces alboniger KCC S-0309 by ammonium sulfate fractionation, heat treatment to eliminate contaminant proteins and chromatography on DEAE-Toyopearl 650S.
S Y, Paik, M, Sugiyama, R, Nomi
openaire   +3 more sources

Protein Disulfide Isomerase Disassembles TDP‐43/G3BP1 Condensates and Antagonizes TDP‐43 Pathological Aggregates

Advanced Science, EarlyView.
Cytoplasmic aggregation of TDP‐43 is a common pathological feature in amyotrophic lateral sclerosis, frontotemporal lobar degeneration, and Alzheimer's disease with TDP‐43 pathology. This study reports that wild‐type PDI slows down phase separation of TDP‐43 through direct interaction with TDP‐43.
Jia‐Qi Liu, Hao Liu, Yu‐Xuan Sun, Yuying Li, Xiangyi Liu, Li‐Qiang Wang, Zhaofei Yang, Qi Fu, Xiaojiao Xu, Jie Chen, Yingshuang Zhang, Jun Zhou, Weidong Le, Mengchao Cui, Yi Liang   +14 more
wiley   +1 more source

ZDHHC9‐Mediated Palmitoylation of ACSL4 Drives Ferroptosis in Diabetes Mellitus–Induced Erectile Dysfunction

Advanced Science, EarlyView.
ABSTRACT Diabetes mellitus‐induced erectile dysfunction (DMED) is a highly prevalent complication among diabetic patients; however, its underlying pathogenic mechanisms remain incompletely understood. Metabolic disorder is a hallmark of diabetes, yet its precise contribution to DMED progression is not well defined.
Wanyang Guo, Ming Xiao, Mengjun Huang, Dongzi Peng, Ruijiang Zeng, Ruilin Liu, Yuanqiao Zhao, Zhihan Ouyang, Yulong Hong, Zexian Ding, Zhuo Xing, Hao Su, Jinxiang Wang, Wenjun Mao, Xin Jin   +14 more
wiley   +1 more source

Puromycin terminated proteins colocalize with ADAM15 and/or PABP at the cell surface.

, 2018
Confocal microscopy of human osteoarthritic chondrocytes double stained with anti-puromycin and anti-ADAM15 antibodies and with anti-puromycin and anti-PABP antibodies and visualized using Alexa 488 (green) and Alexa 594 (red) secondary antibodies ...
Nadine Schneider (1502281), Harald Burkhardt (23413), Beate B. Böhm (5801741), Tomasz Janczi (5801747), Yuliya Fehrl (5801744)   +4 more
core   +1 more source