Results 111 to 120 of about 126,114 (304)

Puromycin-induced retention deficit in goldfish as a function of attained training performance level [PDF]

, 1976
Goldfish received either 20 or 50 active avoidance training trials followed by puromycin (or no-treatment) and 10 retraining trials either 1 or 7 days following training.
Agranoff, Bernard W., Springer, Alan D.
core   +1 more source

PRC2.1 Coordinates Peri‐Nucleolar H3K27me3‐Enriched Heterochromatin Organization and NPM1 Pentamerization to Maintain Nucleolar Integrity

Advanced Science, EarlyView.
PRC2.1(PCL2)‐coordinated H3K27me3‐enriched PNH establishes a spatial scaffold crucial for nucleolar integrity. As a crucial coordinator, PCL2 links PRC2.1 to chromatin organization and NPM1 assembly. This network‐based model reveals how chromatin modifications and nucleolar components cooperatively maintain nucleolar architecture, revealing novel ...
Lina Zhu, Wenqin Wang, Shuyun Chen, Gangyi Zhu, Zhen Wu, Yue Gu, Jingwen Xiong, Xu Zhang, Heng Liu, Kyoichi Isono, Youming Zhang, Jingjing Cao, Xiangzhi Li   +12 more
wiley   +1 more source

Inhibition of SIRT7 Overcomes Radioresistance in Pancreatic Neuroendocrine Tumors by Reactivating MEN1 Expression

Advanced Science, EarlyView.
Pancreatic neuroendocrine tumors frequently silence MEN1 through epigenetic mechanisms. Here, SIRT7 recruits DNMT1 to the MEN1 promoter, drives hypermethylation, and enhances DNA repair. Inhibiting SIRT7 restores MEN1, reduces MRN complex abundance, impairs double‐strand break repair, and sensitizes PanNET models to radiation, supporting SIRT7 as a ...
Jianyun Jiang, Yan Wang, Yi Qin, Luohai Chen, Xiaowu Xu, Guixiong Fan, Desheng Jing, Miaoyan Wei, Xianjun Yu, Junfeng Xu, Shunrong Ji, Jie Chen   +11 more
wiley   +1 more source

An Enhanced Retroviral Vector for Efficient Genetic Manipulation and Selection in Mammalian Cells

Biomolecules
Introducing genetic material into hard-to-transfect mammalian cell lines and primary cells is often best achieved through retroviral infection. An ideal retroviral vector should offer a compact, selectable, and screenable marker while maximizing ...
Jana Triller, Iryna Prots, Hans-Martin Jäck, Jürgen Wittmann   +3 more
doaj   +1 more source

Bi-allelic Homology-Directed Repair with Helper-Dependent Adenoviruses

Molecular Therapy: Methods & Clinical Development, 2019
We describe a strategy to achieve footprintless bi-allelic homology-directed repair (HDR) using helper-dependent adenoviruses (HDAds). This approach utilizes two HDAds to deliver the donor DNA.
Donna J. Palmer, Dustin L. Turner, Philip Ng   +2 more
doaj   +1 more source

G3BP1 Succinylation at K413 is Critical for Cardiac Function by Modulating PI3K‐AKT‐mTOR Signal Axis

Advanced Science, EarlyView.
Schematic illustrating the impact of G3BP1 succinylation at K413 on cardiac function. In the healthy human heart, G3BP1 succinylation maintains homeostatic mTOR signaling. In patients with dilated cardiomyopathy (DCM) and heart failure (HF), G3BP1 de‐succinylation induces RagA expression and disrupts the binding of the TSC1/2 complex, leading to the ...
Yuan Zhang, Cancan Yao, Yan Chen, Ke Cai, Zhouping Lu, Boxuan Wu, Kun Yu, Yan Shi, Jianyuan Zhao, Xiangyu Zhou   +9 more
wiley   +1 more source

Renal structural abnormalities following recovery from acute puromycin nephrosis

, 2002
Renal structural abnormalities following recovery from acute puromycin nephrosis.BackgroundRats that recover from acute puromycin nephrosis later develop widespread glomerular and tubulointerstitial injury. The current study sought to identify structural
Marcussen, Niels, Timothy W. Meyer, Meyer, Timothy W., Jens R. Nyengaard, Ruth Rasch, Rasch, Ruth, Niels Marcussen, Nyengaard, Jens R.   +7 more
core   +1 more source

Tumor‐Derived LAMB3 Drives Immunosuppressive LRRC15+ Fibroblast Formation During Pancreatic Ductal Adenocarcinoma Development

Advanced Science, EarlyView.
A single‐cell atlas of pancreatic ductal adenocarcinoma development reveals progressive ductal‐fibroblast‐immune crosstalk. Tumor‐derived LAMB3 drives the formation of immunosuppressive LRRC15+ fibroblasts through the ITGB1/FAK/MAPK/FOSL2 signaling. Glycolytic reprogramming upregulates LAMB3 and correlates with LRRC15+ fibroblast enrichment.
Xuqing Shi, Hangqi Liu, Jianru Sun, Xiaoding Liu, Xinping Jv, Longyun Chen, Yuhan Zhang, Hui Zhang, Xudong Xing, Ruiyu Li, Xinyi Ke, Jun Wang, Xianglin Yin, Bohan Liu, Qixian Liu, Yuan Wang, Junliang Lu, Shiyi Liu, Junyi Pang, Yumeng Cai, Menghua Dai, Fan Bai, Huanwen Wu, Zhiyong Liang   +23 more
wiley   +1 more source

Effect of Puromycin on Mitotic Index, Amino Acid Incorporation and Dna Synthesis of Epidermal Cells

, 1970
Puromycin 200 mg/kg given systemically will block DNA synthesis, amino acid incorporation and mitosis in the epidermis of the baby rat. Topically applied solutions of 1% puromycin did not have any effect on uptake of H3 thymidine or on mitosis of the ...
Stoughton, Richard B., Robinson, Trevor W.E.   +1 more
core   +1 more source

Sleeping Beauty Transposon-based System for Rapid Generation of HBV-replicating Stable Cell Lines

Bio-Protocol, 2018
The stable HBV-transfected cell lines, which based on stable integration of replication-competent HBV genome into hepatic cells, are widely used in basic research and antiviral drug evaluation against HBV.
Jin-Wei Zheng, Jia-Li Cao, Quan Yuan
doaj   +1 more source