Results 31 to 40 of about 2,825 (207)
Surface Charge Movements of Purple Membrane During Light-Dark Adaptation
The difference in the surface charge distribution between light-adapted and dark-adapted purple membranes was investigated with electric dichroism measurements from approximately pH 5 to pH 11.
Takeuchi, Yasuaki +3 more
core +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
Orthorhombic two-dimensional crystal form of purple membrane
A new two-dimensional crystal form of purple membrane has been obtained in vitro. It is produced by the joint use of a cationic detergent, dodecyltrimethylammonium chloride, and the nonionic detergent, Triton X-100.
Oesterhelt, D. +2 more
core +1 more source
Picosecond events in the photochemical cycle of the light-driven chloride-pump halorhodopsin [PDF]
The early events in halorhodopsin after light excitation are studied with picosecond time resolution. Absorption and fluorescence measurements show that the electronically excited state of the incorporated retinal has a lifetime of 5 ps. Within that time
Hegemann, P. +10 more
core +1 more source
Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth +6 more
wiley +1 more source
Orientation of purple membrane in combined electric and magnetic fields
The orientation of purple membrane in gels for photoelectric measurements is relatively poor, when they are prepared with the standard technique of applying a DC electric field and rapid polymerization. We have improved it by adding a high magnetic field
Kramer, H. +4 more
core +1 more source
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui +10 more
wiley +1 more source
Imaging the surface potential of active purple membrane
Bacteriorhodopsin, the light-driven proton pumping protein in the purple membrane of Halobacterium salinarium, is known to be fully functional in various environments.
Helmut F. Knapp +5 more
core +1 more source
Development of human monoclonal antibodies against TARM1 by yeast display
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe +5 more
wiley +1 more source
Cell membranes separate the inside and outside of cells. Membrane proteins in the cell membrane control the traffic of molecules across the membrane and are therefore targets for a lot of drugs: about 50 % of all approved drugs target a membrane protein!
Scheidelaar, S. +2 more
core

