Pyridoxal 5′-Phosphate-dependent Catalytic Antibody [PDF]
Journal of Biological Chemistry, 1996 Cofactors might efficiently extend the catalytic potential of antibodies. Monoclonal antibodies against Nalpha-phosphopyridoxyl-L-lysine were screened for: 1) binding of 5'-phosphopyridoxyl amino acids, 2) binding of Schiff base of pyridoxal 5'-phosphate (PLP) and amino acids, the first intermediate of all PLP-dependent reactions, and 3) catalysis of ...
S I, Gramatikova, P, Christen
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Pyridoxal 5′-phosphate: electrophilic catalyst extraordinaire [PDF]
Current Opinion in Chemical Biology, 2009 Studies of nonenzymatic electrophilic catalysis of carbon deprotonation of glycine show that pyridoxal 5'-phosphate (PLP) strongly enhances the carbon acidity of alpha-amino acids, but that this is not the overriding mechanistic imperative for cofactor catalysis. Although the fully protonated PLP-glycine iminium ion adduct exhibits an extraordinary low
John P, Richard +3 more
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Vitamin B6 Acquisition and Metabolism in Schistosoma mansoni
Frontiers in Immunology, 2021 Schistosomes are parasitic platyhelminths that currently infect >200 million people globally. The adult worms can live within the vasculature of their hosts for many years where they acquire all nutrients necessary for their survival and growth.
Akram A. Da’dara +4 more
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L-Penicillamine is a mechanism-based inhibitor of serine palmitoyltransferase by forming a pyridoxal-5′-phosphate-thiazolidine adduct [PDF]
, 2012 J. Lowther +4 more
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Pyridoxal-5′-Phosphate-Dependent Alkyl Transfer in Nucleoside Antibiotic Biosynthesis
Nature Chemical Biology, 2020 Several nucleoside antibiotics are structurally characterized by a 5″-amino-5″-deoxyribose (ADR) appended via a glycosidic bond to a high-carbon sugar nucleoside (5′ S ,6′ S )-5′- C -glycyluridine (GlyU).
Z. Cui +11 more
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Pyridoxal in the Cerebrospinal Fluid May Be a Better Indicator of Vitamin B6–dependent Epilepsy Than Pyridoxal 5′-Phosphate [PDF]
, 2020 Background We aimed to demonstrate the biochemical characteristics of vitamin B6–dependent epilepsy, with a particular focus on pyridoxal 5′-phosphate and pyridoxal in the cerebrospinal fluid. Methods Using our laboratory database, we identified patients
Akiyama, Tomoyuki +19 more
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SNZ3 Encodes a PLP Synthase Involved in Thiamine Synthesis in Saccharomyces cerevisiae
G3: Genes, Genomes, Genetics, 2019 Pyridoxal 5′-phosphate (the active form of vitamin B6) is a cofactor that is important for a broad number of biochemical reactions and is essential for all forms of life.
Michael D. Paxhia, Diana M. Downs
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Competition of Pyridoxal 5'-Phosphate with Ribulose 1,5-Bisphosphate and Effector Sugar Phosphates at the Reaction Centers of the Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase [PDF]
, 1980 The Stimulation of the carboxylase reaction by effectors of ribulose 1,5-bisphosphate carboxyl ase/oxygenase displays higher sensitivity towards pyridoxal 5'-phosphate inhibition than the catalytical process itself. Pyridoxal 5'-phosphate binding to the
Gaudszun, Thomas +3 more
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Pyridoxal 5′-Phosphate-Dependent Enzymes at the Crossroads of Host–Microbe Tryptophan Metabolism
International Journal of Molecular Sciences, 2020 The chemical processes taking place in humans intersects the myriad of metabolic pathways occurring in commensal microorganisms that colonize the body to generate a complex biochemical network that regulates multiple aspects of human life.
B. Cellini +6 more
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Studies on the changes in protein fluorescence and enzymic activity of aspartate aminotransferase on binding of pyridoxal 5'-Phosphate [PDF]
, 1974 1. The a and ,B subforms of aspartate aminotransferase were purified from pig heart. 2. The a subform contained 2mol of pyridoxal 5'-phosphate. The apo-(a subform) could be fully reactived by combination with 2mol of cofactor. 3.
Evans, RW, Holbrook, J
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