Results 221 to 230 of about 54,906 (269)
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Fluorometric assay of pyridoxal and pyridoxal 5′-phosphate
Analytical Biochemistry, 1979Abstract A new and very sensitive fluorometric method for the determination of pyridoxal and pyridoxal 5′-phosphate is reported. The specificity is based on the reductive amination of pyridoxal and its 5′-phosphate with methyl anthranilate and sodium cyanoborohydride at pH 4,5 to 5,0.
M S, Chauhan, K, Dakshinamurti
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Biomimetic Chirality Sensing with Pyridoxal-5′-phosphate
Journal of the American Chemical Society, 2017Pyridoxal-5'-phosphate (PLP) is introduced to a biomimetic indicator displacement assay for simultaneous determination of the absolute configuration, enantiomeric composition and concentration of unprotected amino acids, amino alcohols and amines. The chiroptical assay is based on fast imine metathesis with a PLP aryl imine probe to capture the target ...
Christian Wolf
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Spectrophotometric determination of pyridoxal-5-phosphate
Analytica Chimica Acta, 1959Abstract A simple and specific spectrophotometric procedure for the estimation of pyridoxal-5-phosphate is described, based on the complete disappearance of the absorption maximum at 385 mμ following the incubation with potassium cyanide.
V, SCARDI, V, BONAVITA
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Biological role of carbamoyl pyridoxal 5′-phosphate
Comptes Rendus de l'Académie des Sciences - Series III - Sciences de la Vie, 1997A new compound, carbamoyl-pyridoxal 5'-phosphate (C-PLP), was synthetized by condensation of pyridoxal 5'-phosphate (PLP) with KCNO. It may be obtained under certain physiological conditions of pH, temperature and concentration of reagents. Formation and degradation of C-PLP are readily reversible chemical reactions, not involving enzymes, at least in ...
Terzuoli, Lucia +6 more
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Pyridoxal-5′-phosphate and pyridoxal biokinetics in aging Wistar rats
Experimental Gerontology, 1991Biokinetic parameters of plasma pyridoxal-5'-phosphate (PLP) and pyridoxal (PL) disposition were studied in male Wistar rats aged 8 and 27 months kept from weaning on a purified diet containing 250 g casein and 6 mg pyridoxine.HCl per kg. Baseline plasma PLP concentration was lower in the older animals (514 +/- 56 nmol/L for young and 317 +/- 124 nmol ...
Bode, W., Berg, H. van den
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Interaction between Pyridoxal Kinase and Pyridoxal-5-phosphate-Dependent Enzymes
Journal of Biochemistry, 2003The interactions of two pyridoxal-5-phosphate (PLP)-dependent enzymes, alanine aminotransferase (ALT) and glutamate decarboxylase (GAD), with pyridoxal kinase (PK) were studied by fluorescence polarization as well as surface plasmon resonance techniques. The results demonstrated that PK can specifically bind to ALT and GAD.
Pik-Yuen, Cheung +7 more
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Über Pyridoxal‐5′‐phosphat (Codecarboxylase)
Helvetica Chimica Acta, 1951AbstractEs wird eine etwas vereinfachte Darstellung für Pyridoxal‐5′‐phosphat und dessen Magnesium‐ und Calciumsalze beschrieben.
M. Viscontini, C. Ebnöther, P. Karrer
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Polarographische bestimmung des pyridoxals und pyridoxal-5-phosphats
Journal of Electroanalytical Chemistry (1959), 1960The estimation of pyridoxal together with pyridoxal-5-phosphate has been carried out by two polarographic methods. The first method is based upon the varying heights of the pyridoxal waves between ph 2 and 9. Since the height of the wave of the pyridoxal-5-phosphate practically does not alter with the ph, the decrease in the wave height of the mixture ...
Osvald Manoušek, Petr Zuman
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Exploring the pyridoxal 5′‐phosphate‐dependent enzymes
The Chemical Record, 2006AbstractPyridoxal 5′‐phosphate (PLP)‐dependent enzymes represent about 4% of the enzymes classified by the Enzyme Commission. The versatility of PLP in carrying out a large variety of reactions exploiting the electron sink effect of the pyridine ring, the conformational changes accompanying the chemical steps and stabilizing distinct catalytic ...
MOZZARELLI, Andrea, BETTATI, Stefano
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Antibodies against pyridoxal 5′-phosphate and pyridoxamine 5′-phosphate
Biochimica et Biophysica Acta (BBA) - General Subjects, 1966Abstract 1. 1. The antigenicity of pyridoxal 5′-phosphate in rabbits was tested by immunization of the animals with the coenzyme coupled to bovine serum albumin. The coupling of pyridoxal phosphate to albumin, as well as to other proteins, was achieved by reduction (at pH 7·5) with sodium borohydride. 2. 2.
F CORDOBA, C GONZALEZ, P RIVERA
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