Results 1 to 10 of about 21,408 (184)

Protein engineering of NADH pyrophosphatase for efficient biocatalytic production of reduced nicotinamide mononucleotide [PDF]

Frontiers in Bioengineering and Biotechnology, 2023
Introduction: NADH pyrophosphatase, a hydrolase catalyzing the phosphate bond of NADH to reduced nicotinamide mononucleotide, has potential applications in the food, cosmetic and pharmaceutical industry.Methods: Here, we investigated the effects of ...
Ye Liu, Ye Liu, Jin-Song Gong, George Marshall, Chang Su, Chang Su, Michael Hall, Heng Li, Guo-Qiang Xu, Jin-Song Shi, Jin-Song Shi, Zheng-Hong Xu, Zheng-Hong Xu   +12 more
doaj   +2 more sources

Enhancement of Activity of Thermophilic Inorganic Pyrophosphatase Ton1914 via Site-Directed Mutagenesis [PDF]

Biomolecules
Inorganic pyrophosphatase (PPase) is an enzyme that catalyzes the hydrolysis of pyrophosphate (PPi) into two phosphates. Ton1914, a thermophilic inorganic pyrophosphatase derived from Thermococcus onnurineus NA1, has good thermal stability and an ...
Siyao Liu, Xinrui Yang, Renjun Gao, Guiqiu Xie   +3 more
doaj   +2 more sources

Tandem inactivation of inositol pyrophosphatases Asp1, Siw14, and Aps1 illuminates functional redundancies in inositol pyrophosphate catabolism in fission yeast [PDF]

mBio
Inositol pyrophosphates 5-IP7, 1-IP7, and 1,5-IP8 are eukaryal signaling molecules that influence cell physiology, especially phosphate homeostasis. In fission yeast, 1,5-IP8 and 1-IP7 impact gene expression by acting as agonists of RNA 3'-processing and
Beate Schwer, Isabel Prucker, Ana M. Sanchez, Jill Babor, Henning J. Jessen, Stewart Shuman   +5 more
doaj   +2 more sources

Tissue-targeted inorganic pyrophosphate hydrolysis in a fugu5 mutant reveals that excess inorganic pyrophosphate triggers developmental defects in a cell-autonomous manner

Frontiers in Plant Science, 2022
Excess PPi triggers developmental defects in a cell-autonomous manner. The level of inorganic pyrophosphate (PPi) must be tightly regulated in all kingdoms for the proper execution of cellular functions. In plants, the vacuolar proton pyrophosphatase (H+-
Shizuka Gunji, Shizuka Gunji, Kensuke Kawade, Kensuke Kawade, Kensuke Kawade, Hiromitsu Tabeta, Hiromitsu Tabeta, Gorou Horiguchi, Gorou Horiguchi, Akira Oikawa, Akira Oikawa, Mariko Asaoka, Masami Yokota Hirai, Masami Yokota Hirai, Hirokazu Tsukaya, Ali Ferjani   +15 more
doaj   +1 more source

Aggregation-Induced Emission of Au/Ag Alloy Nanoclusters for Fluorescence Detection of Inorganic Pyrophosphate and Pyrophosphatase Activity

Frontiers in Bioengineering and Biotechnology, 2021
The development of sensitive and accurate detection of inorganic pyrophosphate (PPi) and pyrophosphatase activity (PPase) is important as they play vital roles in biological systems.
Zhongli Lei, Jie Zhou, Miao Liang, Yan Xiao, Zhihong Liu   +4 more
doaj   +1 more source

Phosphatase activities and available nutrients in soil aggregates affected by straw returning to a calcareous soil under the maize–wheat cropping system

Frontiers in Environmental Science, 2023
The objective of this study was to investigate the effects of different rates of straw returning on soil aggregate stability, phosphatase activities, and the available nitrogen (N) and phosphorus (P) within different soil aggregate sizes.
Xiang-Jie Lin, Xiang-Jie Lin, Guang-Na Zhang, Zhen Wang, Qing-Dian Han, Peng Leng   +5 more
doaj   +1 more source

The Crucial Role of Soil Organic Matter in Satisfying the Phosphorus Requirements of Olive Trees (Olea europaea L.)

Agriculture, 2021
Under high organic matter content, even under low extractable soil P concentrations, sufficient or over-sufficient foliar P levels may be found. This multi-year study aimed at examining the effects of organic matter content and irrigation management on ...
Niki Christopoulou, Theocharis Chatzistathis, Efimia M. Papatheodorou, Vassilis Aschonitis, Nikolaos Monokrousos   +4 more
doaj   +1 more source

Activities, substrate specificity, and genetic interactions of fission yeast Siw14, a cysteinyl-phosphatase-type inositol pyrophosphatase

mBio, 2023
Inositol pyrophosphate 1,5-IP8 is a signaling molecule that regulates phosphate and polyphosphate homeostasis in the fission yeast Schizosaccharomyces pombe. 1,5-IP8 levels are dictated by a balance between the Asp1 kinase domain that converts 5-IP7 to 1,
Ana M. Sanchez, Beate Schwer, Nikolaus Jork, Henning J. Jessen, Stewart Shuman   +4 more
doaj   +1 more source

A Moonlighting microRNA: Mechanism(s) of miR-122-Mediated Viral RNA Accumulation

Proceedings, 2020
Hepatitis C virus (HCV) is a positive-sense RNA virus that interacts with a human-liver-specific microRNA, termed miR-122. miR-122 binds to two sites in the 5' untranslated region (UTR) of the viral genome, and this interaction promotes HCV RNA ...
Jasmin Chahal, Luca FR Gebert, Hin Hark Gan, Kristin C Gunsalus, Ian J MacRae, Selena M Sagan   +5 more
doaj   +1 more source

Lack of Vacuolar H+ -Pyrophosphatase and Cytosolic Pyrophosphatases Causes Fatal Developmental Defects in Arabidopsis thaliana

Frontiers in Plant Science, 2020
The cytosolic level of inorganic pyrophosphate (PPi) is finely regulated, with PPi hydrolyzed primarily by the vacuolar H+-pyrophosphatase (H+-PPase, VHP1/FUGU5/AVP1) and secondarily by five cytosolic soluble pyrophosphatases (sPPases; PPa1–PPa5) in ...
Mayu Fukuda, Marika Mieda, Ryosuke Sato, Satoru Kinoshita, Takaaki Tomoyama, Ali Ferjani, Masayoshi Maeshima, Masayoshi Maeshima, Shoji Segami, Shoji Segami   +9 more
doaj   +1 more source