Results 41 to 50 of about 21,408 (184)

Thermo Sequenase™ DNA Polymerase and T. acidophilum Pyrophosphatase: New Thermostable Enzymes for DNA Sequencing

BioTechniques, 1997
A combination of thermostable enzymes has been developed that produces higher quality cycle sequences. Thermo Sequenase™ DNA polymerase is a thermostable enzyme engineered to catalyze the incorporation of ddNTPs with an efficiency several thousandfold ...
P.B. Vander Horn, M.C. Davis, J.J. Cunniff, C. Ruan, B.F. McArdle, S.B. Samols, J. Szasz, G. Hu, K.M. Hujer, S.T. Domke, S.R. Brummet, R.B. Moffett, C.W. Fuller   +12 more
doaj   +1 more source

Characterization of a non-nudix pyrophosphatase points to interplay between flavin and NAD(H) homeostasis in Saccharomyces cerevisiae. [PDF]

PLoS ONE, 2018
The flavin cofactors FMN and FAD are required for a wide variety of biological processes, however, little is known about their metabolism. Here, we report the cloning and biochemical characterization of the Saccharomyces cerevisiae pyrophosphatase Fpy1p.
Joseph H Lynch, Na Sa, Sompop Saeheng, Nadia Raffaelli, Sanja Roje   +4 more
doaj   +1 more source

Cleavage-Polyadenylation Factor Cft1 and SPX Domain Proteins Are Agents of Inositol Pyrophosphate Toxicosis in Fission Yeast

mBio, 2022
Inositol pyrophosphate (IPP) dynamics govern expression of the fission yeast phosphate homeostasis regulon via their effects on lncRNA-mediated transcription interference.
Beate Schwer, Angad Garg, Ana M. Sanchez, Mindy A. Bernstein, Bradley Benjamin, Stewart Shuman   +5 more
doaj   +1 more source

TNAP and PHOSPHO1 Function Synergistically to Afford Critical Control Over the Mineralization of the Postnatal Murine Skeleton

Advanced Science, EarlyView.
Biomineralization underpins skeletal development, yet its molecular control remains incompletely understood. Using a novel murine knockout model, this study reveals the essential and complementary roles of PHOSPHO1 and TNAP in postnatal skeletal development.
Lucie E. Bourne, Aikta Sharma, Scott Dillon, Jacob Keen, Soher N. Jayash, Natalie Crump, Lucinda A.E. Evans, Maya Karmali, Worachet Promruk, Claire E. Clarkin, Sonoko Narisawa, Louise Stephen, Brian L. Foster, José Luis Millán, Colin Farquharson, Katherine A. Staines   +15 more
wiley   +1 more source

Purification and pyrophosphatase activity of PHP domain.

, 2016
A) SDS-PAGE of fractions collected during the purification of the E. coli PHP domain. M: molecular mass markers. Lane 1: soluble proteins isolated after refolding of the inclusion bodies containing PHP; lanes 2 and 3: purified PHP domain after ion ...
Massimiliano Lanzi (1463971), Perceval Vellosillo Gonzalez (2601454), Alejandro Hochkoeppler (2082769), Fabio Lapenta (2601445), Sofia Perticarari (2601448), Alejandro Montón Silva (2601451), Renato Brandimarti (617022), Fabio Lino Gratani (2601457)   +7 more
core   +1 more source

Autosomal recessive hypophosphatemic rickets type 2; a novel mutation in the ENPP1 gene

The Turkish Journal of Pediatrics, 2022
Background. Hypophosphatemic rickets (HR) is a rare disease caused by several genetic mutations in factors that cause an increase in fibroblast growth factor 23 (FGF23), and renal phosphate transporters.
Eda Çelebi Bitkin, Huri Sema Aymelek
doaj   +1 more source

Dual Repression by IPA1 Fine‐Tunes OsbZIP79‐Mediated Salt Tolerance in Rice

Advanced Science, EarlyView.
Dual repression by IPA1 fine‐tunes OsbZIP79‐mediated salt tolerance in rice: direct transcriptional inhibition under normal conditions and salt‐induced degradation under stress. This dual mechanism activates OsbZIP79 to regulate Na+/K+ homeostasis and redox balance via downstream genes, enabling optimal salt stress response.
Hui Wang, Bo Liu, Can Hu, Chenfan Zheng, Yuting Wei, Xier Xu, Wei Yang, Yu Fang, Guangle Shen, Pedro García‐Caparros, Conghe Zhang, Qingyao Shu, Meng Jiang   +12 more
wiley   +1 more source

Functional Specialization of Respiratory and Olfactory Mucus Revealed by Proteomic Profiling

International Forum of Allergy &Rhinology, EarlyView.
ABSTRACT Background Mucus overlies the nasal mucosa in the respiratory and olfactory regions, which perform distinct physiological functions. However, proteomic differences between respiratory mucosa (RM) and olfactory cleft (OC) mucus remain unclear.
Anna Kristina Hernandez, Karoline Lantzsch, Romain Topalian, Nadine Gunder, Philipp Hubel, Katharina Schindowski, Jens Pfannstiel, Thomas Hummel   +7 more
wiley   +1 more source

Vacuolar H+-pyrophosphatase AVP1 is involved in amine fungicide tolerance in Arabidopsis thaliana and provides tridemorph resistance in yeast

Frontiers in Plant Science, 2016
Amine fungicides are widely used as crop protectants. Their success is believed to be related to their ability to inhibit postlanosterol sterol biosynthesis in fungi, in particular sterol-Δ8,Δ7-isomerases and sterol-Δ14-reductases, with a concomitant ...
Agustín eHernández, Agustín eHernández, Rosana eHerrera-Palau, Juan Manuel Madroñal, Tomás eAlbi, Guillermo eLopez-Lluch, Jose Roman Perez-Castiñeira, Plácido eNavas, Federico eValverde, Aurelio eSerrano   +9 more
doaj   +1 more source

Genomics-guided analysis of NAD recycling yields functional elucidation of COG1058 as a new family of pyrophosphatases. [PDF]

PLoS ONE, 2013
We have recently identified the enzyme NMN deamidase (PncC), which plays a key role in the regeneration of NAD in bacteria by recycling back to the coenzyme the pyridine by-products of its non redox consumption.
Lucia Cialabrini, Silverio Ruggieri, Marat D Kazanov, Leonardo Sorci, Francesca Mazzola, Giuseppe Orsomando, Andrei L Osterman, Nadia Raffaelli   +7 more
doaj   +1 more source