Results 121 to 130 of about 1,475 (148)

Probing the Allosteric Mechanism in Pyrrolysyl-tRNA Synthetase Using Energy-Weighted Network Formalism

Biochemistry, 2011
Pyrrolysyl-tRNA synthetase (PylRS) is an atypical enzyme responsible for charging tRNA(Pyl) with pyrrolysine, despite lacking precise tRNA anticodon recognition. This dimeric protein exhibits allosteric regulation of function, like any other tRNA synthetases.
Moitrayee, Bhattacharyya, Saraswathi, Vishveshwara   +1 more
openaire   +2 more sources

A Semi-Rationally Engineered Bacterial Pyrrolysyl-tRNA Synthetase Genetically Encodes Phenyl Azide Chemistry

Biotechnology Journal, 2018
The site-specific incorporation of non-canonical amino acids (ncAAs) at amber codons requires an aminoacyl-tRNA synthetase and a cognate amber suppressor tRNA (tRNACUA ). The archaeal tyrosyl-tRNA synthetase from Methanocaldococcus jannaschii and the pyrrolysyl-tRNA synthetase (PylRS) from Methanosarcina mazei have been extensively engineered to accept
Patrik, Fladischer, Alexandra, Weingartner, Johannes, Blamauer, Barbara, Darnhofer, Ruth, Birner-Gruenberger, Tsvetan, Kardashliev, Anna Joelle, Ruff, Ulrich, Schwaneberg, Birgit, Wiltschi   +8 more
openaire   +2 more sources

Crystallographic Studies on Multiple Conformational States of Active-site Loops in Pyrrolysyl-tRNA Synthetase

Journal of Molecular Biology, 2008
Pyrrolysine, a lysine derivative with a bulky pyrroline ring, is the "22nd" genetically encoded amino acid. In the present study, the carboxy-terminal catalytic fragment of Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS) was analyzed by X-ray crystallography and site-directed mutagenesis.
Tatsuo, Yanagisawa, Ryohei, Ishii, Ryuya, Fukunaga, Takatsugu, Kobayashi, Kensaku, Sakamoto, Shigeyuki, Yokoyama   +5 more
openaire   +2 more sources

Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases

Biochemical and Biophysical Research Communications, 2008
We report a method for site-specifically incorporating l-lysine derivatives into proteins in mammalian cells, based on the expression of the pyrrolysyl-tRNA synthetase (PylRS)-tRNA(Pyl) pair from Methanosarcina mazei. Different types of external promoters were tested for the expression of tRNA(Pyl) in Chinese hamster ovary cells.
Takahito, Mukai, Takatsugu, Kobayashi, Nobumasa, Hino, Tatsuo, Yanagisawa, Kensaku, Sakamoto, Shigeyuki, Yokoyama   +5 more
openaire   +2 more sources

Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single- and Multiple-Noncanonical Amino Acid Mutagenesis

2016
To date, the two systems most extensively used for noncanonical amino acid (ncAA) incorporation via orthogonal translation are based on the Methanococcus jannaschii TyrRS/tRNA CUATyr and the Methanosarcina barkeri/Methanosarcina mazei PylRS/tRNA CUAPyl pairs.
Tobias, Baumann, Matthias, Exner, Nediljko, Budisa   +2 more
openaire   +2 more sources

Quadruply orthogonal pyrrolysyl-tRNA synthetase/tRNAPyl pairs in yeast

This study develops quadruply orthogonal PylRS/tRNAPyl tools via semi-rational design and, through SCRaMbLE-based chassis optimization, enables simultaneous incorporation of three distinct non-canonical amino acids in yeast.
openaire   +1 more source

Integrative oncology: Addressing the global challenges of cancer prevention and treatment

Ca-A Cancer Journal for Clinicians, 2022
Jun J Mao,, Msce, Lorenzo Cohen, Karen M Mustian   +2 more
exaly  

Obesity and adverse breast cancer risk and outcome: Mechanistic insights and strategies for intervention

Ca-A Cancer Journal for Clinicians, 2017
Cynthia Morata-Tarifa, Joyce M Slingerland   +1 more
exaly  

Multidisciplinary standards of care and recent progress in pancreatic ductal adenocarcinoma

Ca-A Cancer Journal for Clinicians, 2020
Aaron J Grossberg, William L Hwang, Anirban Maitra   +2 more
exaly  

Oral complications of cancer and cancer therapy

Ca-A Cancer Journal for Clinicians, 2012
Joel B Epstein, Rene-Jean Bensadoun, Andrei Barasch   +2 more
exaly