Results 111 to 120 of about 145,751 (270)

Asymmetric Cu─N─Ru Bridgedsite Nanozyme‐Loaded Injectable Thermogel Boosts Cuproptosis‐Like Death for Multidrug‐Resistant Urinary Tract Infections

Advanced Science, EarlyView.
To address multidrug‐resistant urinary tract infections (MDR‐UTIs), we developed Cu‐ZIF8‐Ru nanozyme featuring an asymmetric Cu─N─Ru catalytic site. This unique structure enhances multienzyme‐mimetic activity, eradicating resistant bacteria by inducing a cuproptosis‐like death pathway through intracellular Cu2+ accumulation and energy depletion.
Guanlin Li, Shike Zhang, Jinggong Liu, Xin Wang, Heng Wang, Hongcan Yang, Yuhao Zhou, Wen Liu, Hongxing Liu, Wenqi Wu   +9 more
wiley   +1 more source

ERN1 KNOCKDOWN AFFECTS THE EXPRESSION OF PDHA1, PDHB, PDHX, DLD, AND DLAT GENES AND MODIFIES THEIR HYPOXIC REGULATION [PDF]

Biotechnologia Acta
Hypoxia and endoplasmic reticulum stress are critical factors affecting tumor growth. Inhibition of ERN1 protein has the ability to suppress glioma cell proliferation and growth. Pyruvate dehydrogenase complex is a important factor for glioma cell growth
Y.P. KHIKHLO, O.V. HALKIN, Y.M. VILETSKA, O.H. MINCHENKO   +3 more
doaj   +1 more source

Bifunctional Artificial Enzymes‐Loaded Microgels With LOX‐ and CAT‐Like Activities for Metabolic Reprogramming and Scarless Wound Repair

Advanced Science, EarlyView.
A bifunctional lactate oxidase‐like and catalase‐like artificial enzyme (Metazyme) is integrated into a rod‐shaped microgel (MetaRgel) to enable cascade lactate oxidation and oxygen regeneration. By reprogramming the wound metabolic microenvironment, MetaRgel alleviates excessive lactate accumulation, oxidative stress, hypoxia, and inflammation ...
Yongyuan Kang, Pai Peng, Liang Song, Qiaoxuan Wang, Zilong Zhong, Dongchao Qiu, Yunfan Liu, Kefei Zhao, Xiaofei Dong, Changyou Gao   +9 more
wiley   +1 more source

Systematic Metabolic Engineering and Model‐Guided Optimization for High‐Level Production of L‐Theanine from Xylose in Escherichia coli

Advanced Science, EarlyView.
This study is pioneering in constructing the shortest known synthetic pathway for L‐theanine production from xylose within E coli. Through comprehensive metabolic engineering strategies, our engineered strain achieved the highest reported L‐theanine titer from xylose, with a titer of 95.42 g/L, and a yield of 0.55 g/g.
Haolin Han, Boyuan Xue, Guangqi Shan, Meng Meng, Shaojie Wang, Haijia Su   +5 more
wiley   +1 more source

Compensatory Interplay Between Clarin‐1 and Clarin‐2 Deafness‐Associated Proteins Governs Phenotypic Variability in Hearing

Advanced Science, EarlyView.
Functional compensation between clarin‐1 and clarin‐2 in cochlear hair cells. Hearing loss associated with CLRN1 mutations shows striking phenotypic variability; however, the underlying mechanisms remain poorly understood. This study reveals that clarin‐1 and clarin‐2 function cooperatively in cochlear hair cells to sustain mechanoelectrical ...
Maureen Wentling, Aïda Yakhlef Sanchez, Nicolas Thelen, Müge Senarisoy, Maria Hogg, Steven Condamine, Andrea Lelli, Emilia Wysocka, Pranav Patni, Sandrine Vitry, Kerem Yasin Yildizhan, Sébastien Le Gal, Sylvie Nouaille, Michael R. Bowl, Marc Thiry, Didier Dulon, Sedigheh Delmaghani, Aziz El‐Amraoui   +17 more
wiley   +1 more source

Mitochondrial thermal proteome profiling reveals aberrant activation of pyruvate dehydrogenase upon cuproptosis

Cell Reports
Summary: Cuproptosis, a copper-induced form of regulated cell death, holds therapeutic promise in cancer but remains mechanistically unclear. We developed Mito-TPCA, a mitochondrial thermal proximity coaggregation strategy combining enzyme-catalyzed ...
Zongyu Huang, Yanjun Liu, Ya Zeng, Jun Wang, Siyuan Sun, Chris Soon Heng Tan, Peng R. Chen   +6 more
doaj   +1 more source

TrxR2 Lactylation Facilitates Mitochondrial Protection and Endothelial Ferroptosis Resistance in Diabetic Cardiomyopathy

Advanced Science, EarlyView.
TrxR2 deletion in diabetic mice suppresses TUFM‐AMPK‐FUNDC1‐dependent mitophagy in endothelial cells, resulting in SCP2 upregulation and mitochondrial translocation of ACSL4. Mitochondrial ACSL4 promotes mitochondrial eicosanoid biosynthesis and ferroptosis, thereby aggravating cardiac microvascular injury and diabetic cardiomyopathy.
Su Li, Muyin Liu, Chao Chen, Xinyan Li, Xiaopei Yan, Wentao Zhu, Wenyan Qiu, Qiyu Li, Xiangyu Sun, Chao Huang, Ming Yin, Zhangwei Chen, Yao Lu, Junbo Ge, Xiangqing Kong, Juying Qian, Yuqiong Chen   +16 more
wiley   +1 more source

Combining Electrochemical Reduction with Biosynthesis for Directed Conversion of CO2 into a Library of C3 Chemicals

Advanced Science, EarlyView.
In the H‐type electrolytic cell, carbon dioxide is reduced to acetic acid via electro‐microbial catalysis. The simply processed acetic acid is further converted through biological fermentation into high‐value‐added products, including acrylic acid, L‐lactic acid, and β‐alanine.
Kaixing Xiao, Shanquan Liang, Xujun Zhao, Zhiyao Peng, Ruoshi Luo, Jikai Zong, Ling Zhou, Yude Su, Dan Wang   +8 more
wiley   +1 more source

Systematic Engineering of Proteases in Saccharopolyspora Spinosa Reveals Synergistic Enhancement of Spinosad Biosynthesis via Substrate Flux Optimization

Advanced Science, EarlyView.
ARTP mutagenesis yielded Saccharopolyspora spinosa mutant D184 with improved extracellular nitrogen utilization. An integrated workflow of protease genetic manipulation, multi‐omics, and rational synergy design pinpointed a pepP‐clpP‐htpX synergistic triangular combination.
Duo Jin, Wangqiong Chen, Danlu Yang, Qing Liu, Baolong Bai, Zirui Dai, Xirong Liu, Zirong Zhu, Jie Rang, Liqiu Xia   +9 more
wiley   +1 more source

Endometrial Epithelial Lactate Deficiency Drives CD8+ T‐Cells Dysregulation in Unexplained Recurrent Implantation Failure

Advanced Science, EarlyView.
Unexplained recurrent implantation failure (RIF) represents a significant clinical challenge. Our results demonstrate that reduced lactate production in the RIF endometrium impairs the suppression of cytotoxic CD8+ T‑cells, allowing their proliferation and thereby disrupting the local immune balance essential for successful embryo implantation.
Yuanlin He, Kang Ke, Qingxia Meng, Yichun Guan, Jichun Tan, Hong Lv, Boxian Huang, Chan Tian, Qinyan Zou, Mingxing An, Yao Gao, Jingjing Chen, Xiaoyu Wei, Xi Wang, Hongxia Ma, Yuan Lin, Feiyang Diao, Yayun Gu, Zhibin Hu   +18 more
wiley   +1 more source