Results 71 to 80 of about 156,917 (287)

Monitoring phosphorylation of the pyruvate dehydrogenase complex [PDF]

Analytical Biochemistry, 2009
The pyruvate dehydrogenase multienzyme complex (PDC) is a key regulatory point in cellular metabolism linking glycolysis to the citric acid cycle and lipogenesis. Reversible phosphorylation of the pyruvate dehydrogenase enzyme is a critical regulatory mechanism and an important point for monitoring metabolic activity.
Matthew J, Rardin, Sandra E, Wiley, Robert K, Naviaux, Anne N, Murphy, Jack E, Dixon   +4 more
openaire   +2 more sources

Identification of functional murine mitochondrial formyl peptides and their effects on myeloid‐derived suppressor cell generation

FEBS Open Bio, EarlyView.
We first identified functional murine mitochondrial N‐formyl peptides (MT‐FPs) and investigated their effects on the in vitro myeloid‐derived suppressor cell (MDSC) generation from bone marrow cells. We demonstrated that MT‐FPs acted directly on bone marrow cells to promote MDSC generation and modulated the polymorphonuclear (PMN)‐MDSC/monocyte (M ...
Miyako Ozawa, Saori Kagoshima, Akira Yamada   +2 more
wiley   +1 more source

Mitochondrial Encephalomyopathy Associated with Pyruvate Dehydrogenase Complex Deficiency: Eight Clinical Cases

Вопросы современной педиатрии, 2021
Background. Defects in pyruvate dehydrogenase complex (PDC), involved in the glycolysis products integration into the cells' energy metabolism, are one of the reasons of mitochondrial pathology development.
Ekaterina A. Nikolaeva, Svetlana Ya. Volgina, Chulpan D. Khaliullina, Sergey V. Bochenkov, Maria A. Danceva   +4 more
doaj   +1 more source

The Urea Carboxylase and Allophanate Hydrolase Activities of Urea Amidolyase Are Functionally Independent [PDF]

, 2016
Urea amidolyase (UAL) is a multifunctional biotin-dependent enzyme that contributes to both bacterial and fungal pathogenicity by catalyzing the ATP-dependent cleavage of urea into ammonia and CO2.
Bahler, Baker, Balotra, Bratlie, Chapman-Smith, Chen, Chen, Cheng, Dumetz, Fan, Fan, Geck, Ghosh, Greenberg, Hettwer, Huang, Ianeselli, James, Kaessmann, Kanamori, Kanamori, Khosla, Kimura, Koonin, Kotlyar, Kozer, Kulkarni, Kulkarni, Kuriyan, Lawrence, Lherbet, Lietzan, Lin, Lorenz, Marsh, Moxley, Navarathna, Navarathna, Ribeiro, Shapir, Shapir, Singh, Spivey, Strope, Thanabalu, Thoden, Thompson, Whitney, Zeczycki   +48 more
core   +2 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Cryo-EM snapshots of a native lysate provide structural insights into a metabolon-embedded transacetylase reaction

Nature Communications, 2021
How is acetyl-CoA produced in the context of the endogenous, eukaryotic pyruvate dehydrogenase complex metabolon? Here the authors dissect the embedded transacetylase reaction through biochemical, cryo-EM, HADDOCKing and molecular dynamics methods.
Christian Tüting, Fotis L. Kyrilis, Johannes Müller, Marija Sorokina, Ioannis Skalidis, Farzad Hamdi, Yashar Sadian, Panagiotis L. Kastritis   +7 more
doaj   +1 more source

Light Activated Induction of Cuproptosis in Resistant Cancer Cells Using Polymeric BODIPY Nanoparticles for Photoactivated Chemotherapy

Advanced Functional Materials, EarlyView.
This article presents a photo‐responsive nanoparticle platform that precisely triggers copper‐dependent cuproptotic cell death in (drug‐resistant) cancer cells. The system remains stable and inactive in the dark but releases cytotoxic species upon red‐light irradiation, achieving potent activity in drug‐resistant breast cancer cells.
Ricarda Zimmermann, Nicolás Montesdeoca, Johannes Karges   +2 more
wiley   +1 more source

Intracellular acetyl phosphate modulates Escherichia coli pyruvate metabolism

Acta Biochimica et Biophysica Sinica
Lysine acetylation has been shown to be an abundant and vital post-translational modification (PTM) that utilizes acetyl phosphate (AcP) as one of the acetyl group donors in bacteria. The pyruvate dehydrogenase (PDH) complex catalyzes the conversion from
Zhang Ling, Shi Hongmei, Liu Zixiang, Gu Jing, Deng Jiaoyu   +4 more
doaj   +1 more source

Characterization of the metabolic shift between oxidative and fermentative growth in Saccharomyces cerevisiae by comparative ¹³C flux analysis

Microbial Cell Factories, 2005
Background One of the most fascinating properties of the biotechnologically important organism Saccharomyces cerevisiae is its ability to perform simultaneous respiration and fermentation at high growth rate even under fully aerobic conditions.
Wittmann Christoph, Frick Oliver
doaj   +1 more source

Global Metabolic Shifts in Age and Alzheimer's Disease Mouse Brains Pivot at NAD+/NADH Redox Sites. [PDF]

, 2019
Age and Alzheimer's disease (AD) share some common features such as cognitive impairments, memory loss, metabolic disturbances, bioenergetic deficits, and inflammation.
Brewer, Gregory J, Dong, Yue
core