Results 251 to 260 of about 1,004,764 (341)

Getting over qPCR’s technical hurdles

BioTechniques, 2013
openaire   +2 more sources

Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening

Advanced Healthcare Materials, EarlyView.
Models of the human skin must combine the relevant biological contents and suitable biomaterials with the correct spatial organization. Performing compound screening on such in vitro models also requires fast and reproducible production methods of the models.
Elisa Lenzi, Nadia Vertti‐Quintero, Julien Husson, Anne‐Laure Bulteau, Carine Nizard, Karl Pays, Sébastien Sart, Charles N. Baroud   +7 more
wiley   +1 more source

Evaluation of the reproducibility and performance characteristics of the Phagomagnetic separation-qPCR assay for rapidly detecting viable <i>Mycobacterium avium</i> subsp. <i>paratuberculosis</i> in bovine milk and feces. [PDF]

Front Vet Sci
Grant IR, Sevilla IA, Molina E, Romero Martinez B, Lorente-Leal V, Thibault-Poisson VC, Cechova M, Köhler H.   +7 more
europepmc   +1 more source

Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling

Advanced Healthcare Materials, EarlyView.
A fully synthetic, compartmentalized 3D kidney disease model is introduced. The kidney model combines a PEG‐based hydrogel matrix with anisotropic, enzymatically degradable rod‐shaped microgels to spatially arrange a triple co‐culture of key renal epithelial, endothelial, and fibroblast cells.
Ninon Möhl, Daphne Bouwens, Johanna Abele, Aline Hans, Tanja Topic, Daniel Günther, Jitske Jansen, Rafael Kramann, Laura De Laporte   +8 more
wiley   +1 more source

Development and validation of a quadruplex qPCR assay for simultaneous detection of GPV, GoCV, TMUV, and GAstV-Ⅱ in geese. [PDF]

Sci Rep
Li M, Wang M, Wang J, Li H, Wang Q, Yan G, Huang Z.   +6 more
europepmc   +1 more source

RT-qPCR v1

, 2020
openaire   +1 more source

Preliminary Study of a Ninj1‐Loaded Bimodal Ultrasound/NIR Fluorescence Targeted Molecular Probe for Diagnosing Early‐Stage Inflammation in Coronary Microvascular Dysfunction

Advanced Healthcare Materials, EarlyView.
Coronary microvascular dysfunction (CMD) targeting remains a challenge for precise diagnosis. This work presents a dual‐modal nanoprobe (T‐IR780‐NBs) that combines ultrasound contrast with near‐infrared fluorescence. This technology utilizes proteomics‐derived antibodies that specifically localize to inflamed and injured cardiac tissue, enabling ...
Xiaohui Xu, Lina Guan, Baihetiya Tayier, Chen Yuan, Shangke Chen, Qi Shi, Yuming Mu   +6 more
wiley   +1 more source

Evaluating Real-Time PCR to Quantify Drosophila suzukii Infestation of Fruit Crops. [PDF]

Insects
Gullickson MG, Averello V, Rogers MA, Hutchison WD, Hegeman A.   +4 more
europepmc   +1 more source

Algorithm Optimization in Methylation Detection with Multiple RT-qPCR

, 2016
Lele Song, Yuemin Li, Jia Jia, Zhou Guangpeng, Jianming Wang, Qian Kang, Peng Jin, Jianqiu Sheng, Guoxiang Cai, Sanjun Cai, Xiaoliang Han   +10 more
openalex   +2 more sources

Click-qPCR: A Simple Tool for Interactive qPCR Data Analysis

BIO-PROTOCOL
Azusa Kubota, Atsushi Tajima
openaire   +1 more source