Molecular Profiling and Precise Diagnosis of Pratylenchus penetrans Infestation in Soil: A qPCR-Based Molecular Approach [PDF]
The Plant Pathology JournalPratylenchus penetrans, an important soil pathogen, has been reported on various crops in the temperate regions of South Korea. In concern, there is an urgent need for a precise, species-specific quantitative polymerase chain reaction (qPCR) kit to ...
Karthi Natesan +5 more
doaj +1 more source
Epithelial-mesenchymal transition-related genes in coronary artery disease
Open Medicine, 2022 Epithelial-mesenchymal transition (EMT) is critical in the development of coronary artery disease (CAD). However, landscapes of EMT-related genes have not been fully established in CAD.
Xu Xiang +4 more
doaj +1 more source
Frequency of shedding of respiratory pathogens in horses recently imported to the United States. [PDF]
, 2018 BackgroundImported horses that have undergone recent long distance transport might represent a serious risk for spreading infectious respiratory pathogens into populations of horses.ObjectiveTo investigate the frequency of shedding of respiratory ...
Boyle +25 more
core +1 more source
Straightforward and sensitive RT-qPCR based gene expression analysis of FFPE samples [PDF]
, 2016 Fragmented RNA from formalin-fixed paraffin-embedded (FFPE) tissue is a known obstacle to gene expression analysis. In this study, the impact of RNA integrity, gene-specific reverse transcription and targeted cDNA preamplification was quantified in terms
Cuvelier, Claude +5 more
core +2 more sources
Using the VALGENT-3 framework to assess the clinical and analytical performance of the RIATOL qPCR HPV genotyping assay [PDF]
, 2019 Background and objective: The VALGENT framework is developed to assess the clinical performance of HPV tests that offer genotyping capability. Samples from the VALGENT-3 panel are used to identify an optimal viral concentration threshold for the RIATOL ...
Arbyn, Marc +6 more
core +1 more source
Biomolecules, 2020 The bacterial diseases of tilapia caused by Streptococcus agalactiae have resulted in the high mortality and huge economic loss in the tilapia industry. Matrix metalloproteinase-9 (MMP-9) may play an important role in fighting infection.
Fu-Rui Liang +5 more
doaj +1 more source
Guangdong nongye kexue, 2023 【Objective】Hybrid snakehead is an important special aquatic fish in China. In order to find out the infection status of hybrid snakehead rhabdovirus (HSHRV), the rhabdovirus disease of hybrid snakehead cultured in the Pearl River Delta was investigated ...
Caiyi WEN +8 more
doaj +1 more source
Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR. [PDF]
, 2011 Telomere length/DNA content has been measured in epidemiological/clinical settings with the goal of testing a host of hypotheses related to the biology of human aging, but often the conclusions of these studies have been inconsistent.
Aviv, Abraham +5 more
core +2 more sources
Journal of Water and Climate Change, 2023 This study assessed the microbial quality of water sources in winter by microbiological methods and molecular-based quantitative polymerase chain reaction (qPCR) and potential future bacterial loads in the Alleben pond based on the increase in water ...
Hatice Aysun Mercimek Takci +3 more
doaj +1 more source
Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India [PDF]
Veterinary World, 2020 Background and Aim: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months.
Preeti Rohilla +4 more
doaj +1 more source