Results 21 to 30 of about 619,072 (268)

Characterization of Matrix Metalloprotease-9 Gene from Nile tilapia (Oreochromis niloticus) and Its High-Level Expression Induced by the Streptococcus agalactiae Challenge

open access: yesBiomolecules, 2020
The bacterial diseases of tilapia caused by Streptococcus agalactiae have resulted in the high mortality and huge economic loss in the tilapia industry. Matrix metalloproteinase-9 (MMP-9) may play an important role in fighting infection.
Fu-Rui Liang   +5 more
doaj   +1 more source

Investigation of Rhabdovirus Infection in Hybrid Snakehead (Channa argus ♂ × Channa maculata ♀) in the Pearl River Delta

open access: yesGuangdong nongye kexue, 2023
【Objective】Hybrid snakehead is an important special aquatic fish in China. In order to find out the infection status of hybrid snakehead rhabdovirus (HSHRV), the rhabdovirus disease of hybrid snakehead cultured in the Pearl River Delta was investigated ...
Caiyi WEN   +8 more
doaj   +1 more source

Microbial water quality of pond Alleben (Gaziantep, Turkey) in winter and climatic changes in the region

open access: yesJournal of Water and Climate Change, 2023
This study assessed the microbial quality of water sources in winter by microbiological methods and molecular-based quantitative polymerase chain reaction (qPCR) and potential future bacterial loads in the Alleben pond based on the increase in water ...
Hatice Aysun Mercimek Takci   +3 more
doaj   +1 more source

Detection of Leptospira in urine of apparently healthy dogs by quantitative polymerase chain reaction in Haryana, India [PDF]

open access: yesVeterinary World, 2020
Background and Aim: Leptospirosis is a zoonotic disease caused by bacteria of the genus Leptospira. The organism can spread through the urine of infected animals, which can get into water or soil and can survive there for weeks to months.
Preeti Rohilla   +4 more
doaj   +1 more source

qPCR, dPCR, NGS – A journey [PDF]

open access: yesBiomolecular Detection and Quantification, 2015
Scientific conferences fulfil many roles, but one of the most important ones is that they help shape the direction in which a scientific discipline grows by promoting person-to-person exchanges of information, ideas and constructive criticisms between scientists from different backgrounds.
Huggett, Jim   +2 more
openaire   +3 more sources

Biochar Enhances Nitrous Oxide Reduction in Acidic but Not in Near-Neutral pH Soil

open access: yesSoil Systems, 2019
We quantified nitrous oxide (N2O) fluxes and total denitrification (N2O + N2) in an acidic (Ferralsol) and a near-neutral pH soil (Cambisol) to determine whether biochar’s alkalinization effect could be the mechanism inducing potential reductions ...
Engil Isadora Pujol Pereira   +4 more
doaj   +1 more source

Semi-Quantitative, Duplexed qPCR Assay for the Detection of Leishmania spp. Using Bisulphite Conversion Technology

open access: yesTropical Medicine and Infectious Disease, 2019
Leishmaniasis is caused by the flagellated protozoan Leishmania, and is a neglected tropical disease (NTD), as defined by the World Health Organisation (WHO).
Ineka Gow   +4 more
doaj   +1 more source

Comparing the stemness and morphobiometry of spermatogonial stem cells from Doom pig on different days of culture

open access: yesCzech Journal of Animal Science, 2020
The present study was conducted to compare the stemness and morphobiometry of spermatogonial stem cells (SSCs) from the Doom pig on different days of culture (9th, 30th and 65th day) for the development of long-term culture method.
Arpana Das   +8 more
doaj   +1 more source

On non-detects in qPCR data [PDF]

open access: yesBioinformatics, 2014
Motivation: Quantitative real-time PCR (qPCR) is one of the most widely used methods to measure gene expression. Despite extensive research in qPCR laboratory protocols, normalization and statistical analysis, little attention has been given to qPCR non-detects—those reactions failing to produce a minimum amount of signal.Results: We show that the ...
Matthew N. McCall   +3 more
openaire   +2 more sources

Quantification of Pneumocystis jirovecii: Cross-Platform Comparison of One qPCR Assay with Leading Platforms and Six Master Mixes

open access: yesJournal of Fungi, 2019
Diagnosis of Pneumocystis jirovecii pneumonia relies on nucleic acid quantification in respiratory samples. Lack of standardization among molecular assays results in significant differences among assays/centers.
Sarah Dellière   +5 more
doaj   +1 more source

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