Results 21 to 30 of about 647,340 (267)
Biotechnology & Biotechnological Equipment, 2021 The microRNAs (miRNAs) are a group of small non-coding RNAs found in both plants and animals. Their functions and target genes have been identified in many plant species.
Özgür Çakır +3 more
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qPCR, dPCR, NGS – A journey [PDF]
Biomolecular Detection and Quantification, 2015 Scientific conferences fulfil many roles, but one of the most important ones is that they help shape the direction in which a scientific discipline grows by promoting person-to-person exchanges of information, ideas and constructive criticisms between scientists from different backgrounds.
Huggett, Jim +2 more
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Biologia Plantarum, 2016 Globally, drought is the main factor that reduces common bean yield. For this reason, breeding alternatives, such as molecular marker-assisted selection, that focus on various functional genes directly involved in the response to water stress, such as ...
G. M. Gutierrez-Benicio +6 more
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On non-detects in qPCR data [PDF]
Bioinformatics, 2014 Motivation: Quantitative real-time PCR (qPCR) is one of the most widely used methods to measure gene expression. Despite extensive research in qPCR laboratory protocols, normalization and statistical analysis, little attention has been given to qPCR non-detects—those reactions failing to produce a minimum amount of signal.Results: We show that the ...
Matthew N. McCall +3 more
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Czech Journal of Animal Science, 2021 Copy numbers of alpha amylase genes (AMY), which encode starch-digesting enzymes, are markedly increased in modern humans and domesticated dogs as an adaptive evolutionary mechanism in response to increased consumption of starch-rich foods acquired ...
Tsukasa Yoshidomi +6 more
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Click-qPCR: A Simple Tool for Interactive qPCR Data Analysis
BIO-PROTOCOLReal-time quantitative PCR (qPCR) is a pivotal technique for analyzing gene expression and DNA copy number variations. However, the limited availability of user-friendly software tools for qPCR data analysis presents a significant challenge for experimental biologists with limited computational skills.
Kubota, Azusa, Tajima, Atsushi
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Journal of Fungi, 2019 Mucormycosis infections are infrequent yet aggressive and serious fungal infections. Early diagnosis of mucormycosis and its discrimination from other fungal infections is required for targeted treatment and more favorable patient outcomes.
Rita Caramalho +10 more
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Application of uMelt SM algorithm in Real-time PCR melting curve analysis [PDF]
مجله بیوتکنولوژی کشاورزی, 2018 One of the challenges in interpreting the results obtained in qPCR is to make sure the amplicons are specific and that the melting curve analysis is used to examine it.
Anahita Kharabi, Abbas Saedi
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DETECTION AND QUANTIFICATION OF MYCOTOXIGENIC FUNGI IN MAIZE BY REAL-TIME PCR [PDF]
Journal of Engineering Science (Chişinău), 2020 The article focuses on real-time PCR detection and quantification of Fusarium spp., potential producer of class B fumonisin mycotoxins in mature corn kernels using primers designed to genes FUM1 and FUM6, involved in mycotoxin biosynthesis. Research have
MITINA, Irina +4 more
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