Results 41 to 50 of about 932,733 (312)

Quantification of Pneumocystis jirovecii: Cross-Platform Comparison of One qPCR Assay with Leading Platforms and Six Master Mixes

Journal of Fungi, 2019
Diagnosis of Pneumocystis jirovecii pneumonia relies on nucleic acid quantification in respiratory samples. Lack of standardization among molecular assays results in significant differences among assays/centers.
Sarah Dellière, Maud Gits-Muselli, P. Lewis White, Carlo Mengoli, Stéphane Bretagne, Alexandre Alanio   +5 more
doaj   +1 more source

Tuz Stresinin Hafifletilmesinde L-Argininin Zea mays’da Antioksidan Enzim Aktivitesi Üzerine Etkisi

Turkish Journal of Agriculture: Food Science and Technology
Arjinin, bitkilerde stres metabolizmasında yer alan çeşitli metabolitlerin öncülüğünü yapan bir aminoasit olmasının yanı sıra, bitkilerin çevresel streslere yanıtını modüle edebilen bir sinyal molekülüdür.
Esra Arslan Yüksel
doaj   +1 more source

Optimasi Primer yang Menargetkan Gen OsAP2, OsERF3 dan OsEREBP2 Menggunakan qPCR

Bioeksperimen: Jurnal Penelitian Biologi, 2022
qPCR merupakan metode yang akurat untuk mendeteksi ekspresi gen. Analisis ekspresi gen pengkode faktor transkripsi pada padi saat ini banyak dilakukan. OsAP2, OsERF3 dan OsEREBP2 merupakan faktor transkripsi pada padi yang termasuk kedalam kelompok AP2 ...
Arifa Rizqi Nafisa, Alfino Sebastian, Putri Wijayanti, Rosy Feraningsih Patigu, Yekti Asih Purwestri   +4 more
doaj   +1 more source

Enhancing gene expression studies in bovine embryos fertilized in vitro: Identifying stable reference genes across blastocysts with different developmental speeds

The Journal of Reproduction and Development
In vitro fertilization (IVF) is crucial for livestock reproduction; however, pregnancy rates after embryo transfer vary depending on the developmental speeds of the embryos. Although quantitative PCR (qPCR) is used to predict developmental potential, its
Sung-Ho KIM, Sang-Yup LEE, Saet-Byul KIM, Tae-Gyun KIM, Min JANG, Sung-Ho YUN, Seong-Eun HEO, Do-Yoon KIM, Seung-Joon KIM, Won-Jae LEE   +9 more
doaj   +1 more source

Development of an Interferon Gamma Response-Related Signature for Prediction of Survival in Clear Cell Renal Cell Carcinoma

Journal of Inflammation Research, 2021
Lixiao Liu,1,* Xuedan Du,2,* Jintao Fang,3,* Jinduo Zhao,1 Yong Guo,3 Ye Zhao,1 Chengyang Zou,1 Xiaojian Yan,1 Wenfeng Li2 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou ...
Liu L, Du X, Fang J, Zhao J, Guo Y, Zhao Y, Zou C, Yan X, Li W   +8 more
doaj  

Analysis of variance components reveals the contribution of sample processing to transcript variation [PDF]

, 2009
The proper design of DNA microarray experiments requires knowledge of biological and technical variation of the studied biological model. For the filamentous fungus Aspergillus niger, a fast, quantitative real-time PCR (qPCR)-based hierarchical ...
Veen, D., van der, Oliveira, J.M., Berg, W.A.M., van den, Graaff, L.H., de   +3 more
core   +1 more source

Broad Geographic and Host Distribution of Apis mellifera Filamentous Virus in South American Native Bees

Journal of Apicultural Science, 2019
Apis mellifera filamentous virus (AmFV) is a large double stranded DNA virus of honey bees and its prevalence and relationship with other parasites is poorly known.
Quintana Silvina, de Landa Gregorio Fernandez, Revainera Pablo, Meroi Facundo, Porrini Leonardo, Di Geronimo Vanesa, Brasesco Constanza, Plischuk Santiago, Eguaras Martín J., Maggi Matias   +9 more
doaj   +1 more source

Efficacy of Mycoplasma hyopneumoniae vaccination before and at weaning against experimental challenge infection in pigs [PDF]

, 2016
Background: Commercial bacterins are widely used at weaning to control Mycoplasma hyopneumoniae infections in pigs. However, it is not known whether the efficacy of vaccinating against M.
Arsenakis, Ioannis, Boyen, Filip, Del Pozo Sacristán, Rubén, Haesebrouck, Freddy, Maes, Dominiek, Michiels, Annelies, Panzavolta, Luca   +6 more
core   +2 more sources

SPUD qPCR Assay Confirms PREXCEL-Q Software's Ability to Avoid qPCR Inhibition [PDF]

Current Issues in Molecular Biology, 2010
Real-time quantitative polymerase chain reaction is subject to inhibition by substances that co-purify with nucleic acids during isolation and preparation of samples. Such materials alter the activity of reverse transcriptase (RT) and thermostable DNA polymerase enzymes on which the assay depends.
Gallup, Jack, Sow, Fatoumata, Van Geelen, Albert, Ackermann, Mark   +3 more
openaire   +3 more sources

A beginner’s guide to RT-PCR, qPCR and RT-qPCR [PDF]

The Biochemist, 2020
The development of the polymerase chain reaction (PCR), for which Kary Mullis received the 1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that prize was awarded, research was being carried out by Russel Higuchi which led to the discovery that PCR can be monitored using fluorescent probes, facilitating quantitative ...
openaire   +1 more source