Results 41 to 50 of about 647,340 (267)

Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR

The Plant Pathology Journal, 2019
The soybean cyst nematode, Heterodera glycines, is a major plant-parasitic nematode that has caused important economic losses to Korea's soybean production. Four species of cyst nematodes, H. schachtii, H. glycines, H. trifolii, and H.
Hyoung-Rai Ko, Heonil Kang, Eun-Hyoung Park, Eun-Hwa Kim, Jae-Kook Lee   +4 more
doaj   +1 more source

Longitudinal study on oral shedding of human betaherpesviruses 6 and 7 in renal transplant recipients reveals active replication

Journal of Oral Microbiology, 2020
Backgroung Roseolovirus latency and persistence in salivary glands that are frequently reactivated after renal transplantation to cause infection have been reported.
Jéssica Vasques Raposo, Dmitry José De Santana Sarmento, Rafaela Barbosa Da Silva Pinto, Amanda Oliveira Lopes, Marina Gallottini, Tânia Regina Tozetto-Mendoza, Paulo Henrique Braz-Silva, Vanessa Salete de Paula   +7 more
doaj   +1 more source

pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis

Shipin gongye ke-ji, 2023
The effects of citric acid addition coupled with pH on cell growth, metabolism, and spore yield of B. licheniformis were studied in a 30 L bioreactor, and the gene expression differences related to energy metabolism, oxidative stress effect, and amino ...
Yue DING, Junfeng HE, Ruofei GONG, Hang DAI, Xiong CHEN, Yanan HUANG, Zhi WANG   +6 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

The kinetic requirements of extreme qPCR

Biomolecular Detection and Quantification, 2019
The kinetic requirements of quantitative PCR were experimentally dissected into the stages of DNA denaturation, primer annealing, and polymerase extension. The temperature/time conditions for 2 stages were kept optimal, while the other was limited until the amplification efficiency decreased as measured by an increase in quantification cycle (Cq ...
Adam L. Millington, Jessica A. Houskeeper, John F. Quackenbush, James M. Trauba, Carl T. Wittwer   +4 more
openaire   +2 more sources

Partial depletion of plasminogen activator inhibitor‐1 decreases subcutaneous fat cell hypertrophy and liver cholesterol in high‐fat‐fed female mice

FEBS Letters, EarlyView.
Obesity raises blood levels of PAI‐1, a protein linked to metabolic dysfunction‐associated steatotic liver disease in people with obesity. In female mice fed a high‐fat diet, partially lowering PAI‐1 led to smaller subcutaneous fat cells and lower liver cholesterol, without changing body weight or insulin sensitivity.
Claudia E. Ramirez Bustamante, Robert Sharp, Pradip K. Saha, Elizabeth S. Anaya, Elizabeth L. Lieu, Evelyn L. de Groot, Pooja Patel, Mi Jeong Heo, Aaron R. Cox, Kang Ho Kim, Sean M. Hartig   +10 more
wiley   +1 more source

High occurrence of viable forms of Cryptosporidium and Giardia in domestic sewage from an agricultural region of Brazil

Journal of Water and Health, 2022
Cryptosporidium and Giardia are the main etiologies of waterborne outbreaks caused by protozoa. These parasites are commonly detected in wastewater; however, there is little knowledge about the concentration of viable forms in treated sewage, mainly in ...
Winni Alves Ladeia, Felippe Danyel Cardoso Martins, Beatriz de Souza Lima Nino, Arielle da Cunha Silvério, Ana Clécia da Silva, Raul Ossada, Douglas Aparecido da Silva, João Luis Garcia, Roberta Lemos Freire   +8 more
doaj   +1 more source

Developmental programmes drive cellular plasticity, disease progression and therapy resistance in lung adenocarcinoma

Molecular Oncology, EarlyView.
This study shows that lung adenocarcinomas exploit developmental branching morphogenesis to acquire a therapy resistant basal‐like tumour cell state. This process was found to be regulated by combined TP53 loss‐of‐function and type‐I interferon signalling, identifying a novel axis for biomarker and therapeutic target discovery.
Kamila J Bienkowska, Stephany Gallardo, Nur S. Zainal, Leena Arora, Matthew Ellis, Maria‐Antoinette Lopez, Judith Austine, Sai Pittla, Serena J Chee, Aiman Alzetani, Emily C Shaw, Christian H Ottensmeier, Gareth J Thomas, Christopher J Hanley   +13 more
wiley   +1 more source

Stimulator of interferon genes agonist augmented antitumor immunity of osimertinib in Egfr‐mutated lung cancer

Molecular Oncology, EarlyView.
Combining osimertinib with the STING agonist ADU‐S100 activates innate and adaptive immunity to overcome the non‐inflamed microenvironment of Egfr‐mutant lung cancer. This combination increases NK and CD8+ T‐cell infiltration, associated with activation of the STING‐IRF3 pathway and local immunogenic cell death.
Jun Nishimura, Tadahiro Kuribayashi, Johannes Brägelmann, Sachi Okawa, Masataka Taoka, Shunta Mori, Tomoka Nishimura, Takaaki Tanaka, Go Makimoto, Kiichiro Ninomiya, Kammei Rai, Eiki Ichihara, Ryohei Katayama, Katsuyuki Hotta, Masahiro Tabata, Yosuke Togashi, Yoshinobu Maeda, Martin L. Sos, Katsuyuki Kiura, Kadoaki Ohashi   +19 more
wiley   +1 more source

qPCR primer design revisited

Biomolecular Detection and Quantification, 2017
Primers are arguably the single most critical components of any PCR assay, as their properties control the exquisite specificity and sensitivity that make this method uniquely powerful. Consequently, poor design combined with failure to optimise reaction conditions is likely to result in reduced technical precision and false positive or negative ...
Bustin, Stephen A., Huggett, Jim
openaire   +2 more sources