Results 31 to 40 of about 647,340 (267)

The 'PREXCEL-Q Method' for qPCR

International Journal of Biomedical Science, 2008
The purpose of this manuscript is to describe a reliable approach to quantitative real-time polymerase chain reaction (qPCR) assay development and project management, which is currently embodied in the Excel 2003-based software program named 'PREXCEL-Q' (P-Q) (formerly known as 'FocusField2-6Gallup-qPCRSet-upTool-001,' 'FF2-6-001 qPCR set-up tool' or ...
Ackermann, Mark, Gallup, Jack
openaire   +3 more sources

Identification and Analysis Long Noncoding RNA in Response to Cold Stress in Cucumber

Journal of the American Society for Horticultural Science
Long noncoding RNAs (lncRNAs) are crucial components of plant genomes, often exceeding the number of protein-coding genes. Although recent studies have highlighted the importance of plant lncRNAs in low-temperature responses, the identification and ...
Yang Liu, Sheng Sun, Chang Lu
doaj   +1 more source

Co-Acquired Nanovirus and Geminivirus Exhibit a Contrasted Localization within Their Common Aphid Vector

Viruses, 2020
Single-stranded DNA (ssDNA) plant viruses belong to the families Geminiviridae and Nanoviridae. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner.
Jérémy Di Mattia, Faustine Ryckebusch, Marie-Stéphanie Vernerey, Elodie Pirolles, Nicolas Sauvion, Michel Peterschmitt, Jean-Louis Zeddam, Stéphane Blanc   +7 more
doaj   +1 more source

An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane

FEBS Letters, EarlyView.
Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa, Akira Hattori, Masafumi Tsujimoto, Toshihide Kobayashi   +3 more
wiley   +1 more source

Optimization and validation of a real-time PCR method for the simultaneous detection of Lactococcus garvieae and Streptococcus agalactiae in fish

Journal of BioScience and Biotechnology
Background: Lactococcus garvieae and Streptococcus agalactiae infections contribute to heavy losses in aquaculture farms worldwide. Currently, available pathogen diagnostic tools use biochemical and microbiological methods beleaguered by very low ...
Taona Zinyakasa, Farisai Chidzwondo, Tatenda Makawa, Sitokozile Sibanda, Exnevia Gomo, Tivapasi Musa, Elizabeth Gori   +6 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Phenotypic analysis of extracellular vesicles: a review on the applications of fluorescence

Journal of Extracellular Vesicles, 2020
Extracellular vesicles (EVs) have numerous potential applications in the field of healthcare and diagnostics, and research into their biological functions is rapidly increasing.
Maria S. Panagopoulou, Alastair W. Wark, David J S Birch, Christopher D. Gregory   +3 more
doaj   +1 more source

Modelling stem cell differentiation related processes—A practical overview for biologists

FEBS Letters, EarlyView.
Stem cell differentiation is complex and difficult to control experimentally. This review introduces suitable computational modelling approaches that can support stem cell research, from mechanistic ODE and abstract models to multiscale and deep learning methods.
Ricco Zeegelaar, Xinqi Yan, Lucas F. Jansen Klomp, Stefano Schivo, Janine N. Post   +4 more
wiley   +1 more source

A one-step multiplex qPCR assay for simultaneous identification and quantification of Leishmania martiniquensis and Leishmania orientalis/Leishmania chancei and detection and quantification of trypanosomatids in clinical samples

Parasite
Leishmaniasis is one of the most important zoonotic diseases. Recently, Leishmania (Mundinia) martiniquensis, Leishmania (Mundinia) orientalis, and Leishmania (Mundinia) chancei have been reported as new human pathogens.
Mano Chonlada, Hirunpatrawong Piyapha, Prasertsilp Patiya, Kaewmee Saowalak, Limprasert Parinda, Siriyasatien Padet, Tantiworawit Adisak, Gatherer Derek, Urbaniak Michael D., Bates Paul A., Jariyapan Narissara   +10 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source