Results 31 to 40 of about 623,101 (265)
The 'PREXCEL-Q Method' for qPCR
International Journal of Biomedical Science, 2008 The purpose of this manuscript is to describe a reliable approach to quantitative real-time polymerase chain reaction (qPCR) assay development and project management, which is currently embodied in the Excel 2003-based software program named 'PREXCEL-Q' (P-Q) (formerly known as 'FocusField2-6Gallup-qPCRSet-upTool-001,' 'FF2-6-001 qPCR set-up tool' or ...
Ackermann, Mark, Gallup, Jack
openaire +3 more sources
Clinical biochemical significance of STAT6, ERG, and miR-647 expression in prostate cancer: Associations with tumor aggressiveness and patient prognosis [PDF]
Journal of Medical BiochemistryBackground: Reliable molecular indicators that can facilitate early detection and prognosis prediction in prostate cancer are still insufficient. Although Transcription 6 (STAT6), ERG, and microRNA-647 (miR-647) have recently been recognized as key ...
Hong Xin +4 more
doaj +1 more source
Application of uMelt SM algorithm in Real-time PCR melting curve analysis [PDF]
مجله بیوتکنولوژی کشاورزی, 2018 One of the challenges in interpreting the results obtained in qPCR is to make sure the amplicons are specific and that the melting curve analysis is used to examine it.
Anahita Kharabi, Abbas Saedi
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FEBS Letters, EarlyView.Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi +5 more
wiley +1 more source
Identification and Analysis Long Noncoding RNA in Response to Cold Stress in Cucumber
Journal of the American Society for Horticultural ScienceLong noncoding RNAs (lncRNAs) are crucial components of plant genomes, often exceeding the number of protein-coding genes. Although recent studies have highlighted the importance of plant lncRNAs in low-temperature responses, the identification and ...
Yang Liu, Sheng Sun, Chang Lu
doaj +1 more source
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
Journal of BioScience and BiotechnologyBackground: Lactococcus garvieae and Streptococcus agalactiae infections contribute to heavy losses in aquaculture farms worldwide. Currently, available pathogen diagnostic tools use biochemical and microbiological methods beleaguered by very low ...
Taona Zinyakasa +6 more
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An isoform of 14‐3‐3 protein regulates transbilayer lipid movement at the plasma membrane
FEBS Letters, EarlyView.Loss of 14‐3‐3ζ in CHO cells confers resistance to exogenous phosphatidylserine (PS) and impairs endocytosis‐independent inward flip‐flop of fluorescent PS at the plasma membrane. RNAi‐mediated knockdown reproduces this defect, while no additive effect is seen in ATP11C‐deficient cells.
Akiko Yamaji‐Hasegawa +3 more
wiley +1 more source
Viruses, 2020 Single-stranded DNA (ssDNA) plant viruses belong to the families Geminiviridae and Nanoviridae. They are transmitted by Hemipteran insects in a circulative, mostly non-propagative, manner.
Jérémy Di Mattia +7 more
doaj +1 more source
pH Coupled Citric Acid Feeding Strategy to Promote Spore Production of Bacillus licheniformis
Shipin gongye ke-ji, 2023 The effects of citric acid addition coupled with pH on cell growth, metabolism, and spore yield of B. licheniformis were studied in a 30 L bioreactor, and the gene expression differences related to energy metabolism, oxidative stress effect, and amino ...
Yue DING +6 more
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