DNA-based faecal dietary analysis: A comparison of qPCR and high throughput sequencing approaches [PDF]
, 2011 The genetic analysis of faecal material represents a relatively non-invasive way to study animal diet and has been widely adopted in ecological research.
Murray, D.C. +8 more
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Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
, 2020 BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer +3 more
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Journal of Water and Health, 2022 Limited information exists on the environmental persistence of genetic markers for fecal indicator bacteria (FIB) in treated wastewaters. Here, the decay rate constants of culturable cells and genetic markers for four diverse groups of FIBs, such as ...
Eunice C. Chern +4 more
doaj +1 more source
Universal probe-based intermediate primer-triggered qPCR (UPIP-qPCR) for SNP genotyping [PDF]
BMC Genomics, 2021 AbstractBackgroundThe detection and identification of single nucleotide polymorphism (SNP) is essential for determining patient disease susceptibility and the delivery of medicines targeted to the individual. At present, SNP genotyping technology includes Sanger sequencing, TaqMan-probe quantitative polymerase chain reaction (qPCR), amplification ...
Baowei Li +10 more
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Учёные записки Казанского университета: Серия Естественные науки, 2019 Phytopathogenic strains of Fusarium oxysporum can parasitize on more than 120 species of crops and ornamental plants posing a serious danger to world agriculture. The wide range of hosts can be evidence of fast changes in the genomes of F.
D.M. Afordoanyi +2 more
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Inducible versus constitutive social immunity: examining effects of colony infection on glucose oxidase and defensin-1 production in honeybees [PDF]
Royal Society Open Science, 2017 Honeybees use a variety of defence mechanisms to reduce disease infection and spread throughout the colony. Many of these defences rely on the collective action of multiple individuals to prevent, reduce or eradicate pathogens—often referred to as ...
Margarita M. López-Uribe +2 more
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Amplification efficiency and thermal stability of qPCR instrumentation: Current landscape and future perspectives [PDF]
, 2015 This is a final draft version of the publication following acceptance by the journal.Quantitative polymerase chain reaction (qPCR) is a method of amplifying and detecting small samples of genetic material in real time and is in routine use across many ...
Karteris, E, Rogers-Broadway, KR
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Liver Molecular-biochemical Markers in Viral Hepatitis B patients
Zanco Journal of Pure and Applied Sciences, 2021 The prevalence of liver disease has led to its emergence as a major challenge. This challenge is also attributed to factors such as the challenging diagnosis, complexity of pathogenesis, and the absence of established therapies.
Lana Basil Khudhur, Fakhir Najim
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Comparison of droplet digital PCR and qPCR for the quantification of Shiga toxin-producing Escherichia coli in bovine feces [PDF]
, 2016 Cattle are considered to be the main reservoir for Shiga toxin-producing Escherichia coli (STEC) and are often the direct or indirect source of STEC outbreaks in humans.
De Reu, Koen +5 more
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Multiplex quantitative PCR for single-reaction genetically modified (GM) plant detection and identification of false-positive GM plants linked to Cauliflower mosaic virus (CaMV) infection. [PDF]
, 2019 BACKGROUND:Most genetically modified (GM) plants contain a promoter, P35S, from the plant virus, Cauliflower mosaic virus (CaMV), and many have a terminator, TNOS, derived from the bacterium, Agrobacterium tumefaciens. Assays designed to detect GM plants
A Bak +35 more
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