Results 11 to 20 of about 647,340 (267)
Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data [PDF]
Scientific Reports, 2021 AbstractQuantifying changes in DNA and RNA levels is essential in numerous molecular biology protocols. Quantitative real time PCR (qPCR) techniques have evolved to become commonplace, however, data analysis includes many time-consuming and cumbersome steps, which can lead to mistakes and misinterpretation of data. To address these bottlenecks, we have
Gilles Maussion +13 more
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A beginner’s guide to RT-PCR, qPCR and RT-qPCR [PDF]
The Biochemist, 2020 The development of the polymerase chain reaction (PCR), for which Kary Mullis received the 1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that prize was awarded, research was being carried out by Russel Higuchi which led to the discovery that PCR can be monitored using fluorescent probes, facilitating quantitative ...
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qPCR as a Selective Tool for Cytogenetics
Plants, 2022 qPCR is widely used in quantitative studies of plant genomes and transcriptomes. In this article, this method is considered as an auxiliary step in the preparation and selection of markers for FISH analysis. Several cases from the authors’ research on populations of the same species were reviewed, and a comparison of the closely related species, as ...
Mikhail G. Divashuk +7 more
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Biobran/MGN-3: A Promising Natural Strategy for Mitigating Kidney Damage and Immune Modulation During Etoposide Therapy [PDF]
Journal of Bioscience and Applied ResearchAlthough chemotherapy is one of the pillars in cancer treatment, its side effects have extremely harmful impacts on many of the body's organs. This study investigates the potential protective effect of the natural product, Biobran/MGN-3, against kidney ...
Ali Eldib +10 more
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Detection of Weligama Coconut Leaf Wilt Disease Phytoplasma by Real-Time Polymerase Chain Reaction
CORD, 2020 Weligama Coconut Leaf Wilt Disease (WCLWD) is a non-lethal, but debilitating phytoplasma disease found in coconut palms in Sri Lanka which is confined to the Southern Province of the country, well-away from the major coconut growing area.
H.T.R. Wijesekara +4 more
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Journal of Pure and Applied Microbiology, 2022 The advent of technologies has made allogenic transplantation a potential curative therapy for end-stage renal diseases, but the episodes of rejection still remain as one of the challenges in the post-transplant scenario.
C. Priscilla +5 more
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Molecular Detection of Ehrlichia canis in the Pet-Dog Population in R. N. Macedonia
Acta Veterinaria, 2021 Canine monocytic ehrlichiosis (CME) is a widespread, tick-borne, canine disease, caused by an obligate intracellular bacterium, Ehrlichia canis. The main vector, a brown-dog tick, Rhipicephalus sanguineus, is widely distributed, especially in areas with ...
Petrov Elena Atanaskova +4 more
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Validation of kinetics similarity in qPCR [PDF]
Nucleic Acids Research, 2011 Quantitative real-time PCR (qPCR) is the method of choice for specific and sensitive quantification of nucleic acids. However, data validation is still a major issue, partially due to the complex effect of PCR inhibition on the results. If undetected PCR inhibition may severely impair the accuracy and sensitivity of results. PCR inhibition is addressed
Bar, T. +2 more
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A Preliminary Survey of Cultured Fusaria from Symptomatic Legume Grains in North-Eastern Poland
Toxins, 2019 Legumes are amongst the most promising crops to satisfy the increasing demand for protein-rich food and feed. Today, however, their cultivation in Europe is low, while European agriculture faces a deficit of protein-rich feed, of which the largest part ...
Maciej Żelechowski +2 more
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Revista Peruana de Biología, 2020 Production of lignocellulolytic enzymes by filamentous fungi have a great potential at industrial level due to their widespread applications. Mixed fungal cultures and particularly mixed fungal biofilms constitute a promising fermentation system for an ...
Alonso Reyes-Calderón +5 more
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