Results 11 to 20 of about 619,072 (268)
Auto-qPCR; a python-based web app for automated and reproducible analysis of qPCR data [PDF]
Scientific Reports, 2021 AbstractQuantifying changes in DNA and RNA levels is essential in numerous molecular biology protocols. Quantitative real time PCR (qPCR) techniques have evolved to become commonplace, however, data analysis includes many time-consuming and cumbersome steps, which can lead to mistakes and misinterpretation of data. To address these bottlenecks, we have
Gilles Maussion +13 more
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Journal of Water and Health, 2022 Limited information exists on the environmental persistence of genetic markers for fecal indicator bacteria (FIB) in treated wastewaters. Here, the decay rate constants of culturable cells and genetic markers for four diverse groups of FIBs, such as ...
Eunice C. Chern +4 more
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Учёные записки Казанского университета: Серия Естественные науки, 2019 Phytopathogenic strains of Fusarium oxysporum can parasitize on more than 120 species of crops and ornamental plants posing a serious danger to world agriculture. The wide range of hosts can be evidence of fast changes in the genomes of F.
D.M. Afordoanyi +2 more
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Inducible versus constitutive social immunity: examining effects of colony infection on glucose oxidase and defensin-1 production in honeybees [PDF]
Royal Society Open Science, 2017 Honeybees use a variety of defence mechanisms to reduce disease infection and spread throughout the colony. Many of these defences rely on the collective action of multiple individuals to prevent, reduce or eradicate pathogens—often referred to as ...
Margarita M. López-Uribe +2 more
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Liver Molecular-biochemical Markers in Viral Hepatitis B patients
Zanco Journal of Pure and Applied Sciences, 2021 The prevalence of liver disease has led to its emergence as a major challenge. This challenge is also attributed to factors such as the challenging diagnosis, complexity of pathogenesis, and the absence of established therapies.
Lana Basil Khudhur, Fakhir Najim
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A beginner’s guide to RT-PCR, qPCR and RT-qPCR [PDF]
The Biochemist, 2020 The development of the polymerase chain reaction (PCR), for which Kary Mullis received the 1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that prize was awarded, research was being carried out by Russel Higuchi which led to the discovery that PCR can be monitored using fluorescent probes, facilitating quantitative ...
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Molecular Profiling and Precise Diagnosis of Pratylenchus penetrans Infestation in Soil: A qPCR-Based Molecular Approach [PDF]
The Plant Pathology JournalPratylenchus penetrans, an important soil pathogen, has been reported on various crops in the temperate regions of South Korea. In concern, there is an urgent need for a precise, species-specific quantitative polymerase chain reaction (qPCR) kit to ...
Karthi Natesan +5 more
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Epithelial-mesenchymal transition-related genes in coronary artery disease
Open Medicine, 2022 Epithelial-mesenchymal transition (EMT) is critical in the development of coronary artery disease (CAD). However, landscapes of EMT-related genes have not been fully established in CAD.
Xu Xiang +4 more
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Validation of kinetics similarity in qPCR [PDF]
Nucleic Acids Research, 2011 Quantitative real-time PCR (qPCR) is the method of choice for specific and sensitive quantification of nucleic acids. However, data validation is still a major issue, partially due to the complex effect of PCR inhibition on the results. If undetected PCR inhibition may severely impair the accuracy and sensitivity of results. PCR inhibition is addressed
Bar, T. +2 more
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qPCR as a Selective Tool for Cytogenetics
Plants, 2022 qPCR is widely used in quantitative studies of plant genomes and transcriptomes. In this article, this method is considered as an auxiliary step in the preparation and selection of markers for FISH analysis. Several cases from the authors’ research on populations of the same species were reviewed, and a comparison of the closely related species, as ...
Mikhail G. Divashuk +7 more
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