Results 131 to 140 of about 1,617,447 (322)

Validation of Reference Genes for Normalization of Relative qRT-PCR Studies in Papillary Thyroid Carcinoma

Scientific Reports, 2019
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) in thyroid tumors require accurate data normalization, however, there are no sufficient studies addressing the suitable reference genes for gene expression analysis in malignant and ...
S. A. Razavi, S. A. Razavi, M. Afsharpad, M. Modarressi, M. Zarkesh, P. Yaghmaei, S. Nasiri, S. Tavangar, Hanieh Gholami, Afsoon Daneshafrooz, M. Hedayati   +10 more
semanticscholar   +1 more source

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

Differential regulation of ZFAS1 splice variants by endoplasmic reticulum stress in hepatocyte cell lines

FEBS Open Bio, EarlyView.
ZFAS1 is a lncRNA promoting cell proliferation and migration, exhibiting high expression in various cancers. It is conserved, widely expressed, and produces multiple splice variants with unclear roles. We identified several splice variants in hepatocyte models, and found that inhibiting or suppressing regulators of the unfolded protein response (PERK ...
Sébastien Soubeyrand, Paulina Lau, Ruth McPherson   +2 more
wiley   +1 more source

Reference genes for qRT-PCR normalisation in different tissues, developmental stages, and stress conditions of Hypericum perforatum

PeerJ, 2019
Hypericum perforatum L. is a widely known medicinal herb used mostly as a remedy for depression because it contains high levels of naphthodianthrones, phloroglucinols, alkaloids, and some other secondary metabolites.
Wen Zhou, Shiqiang Wang, Lei Yang, Yan Sun, Qian Zhang, Bin Li, Bin Wang, Lin Li, Donghao Wang, Zhe-zhi Wang   +9 more
semanticscholar   +1 more source

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Expression of two genes of paclitaxel biosynthetic pathway during germination of Taxus baccata zygotic embryos

Biologia Plantarum, 2010
The spatial and temporal expression of dbat and dbtnbt genes involved in the later steps of paclitaxel biosynthesis in relation to baccatin III and paclitaxel accumulation in Taxus baccata L. germinating embryos and seedlings was investigated. The steady-
K. Bruňáková, J. Košuth, Z. Katkovčinová, M. Lázárová, E. Čellárová   +4 more
doaj   +1 more source

Cellular bases of the RNA metabolism dysfunction in motor neurons of a murine model of spinal muscular atrophy: Role of Cajal bodies and the nucleolus [PDF]

, 2017
Spinal muscular atrophy (SMA) is caused by a homozygous deletion or mutation in the survival motor neuron 1 (SMN1) gene that leads to reduced levels of SMN protein resulting in degeneration of motor neurons (MNs).
Berciano, Maria T., Calderó i Pardo, Jordi, Lafarga, Miguel, Narcís, Oriol J., Piedrafita Llorens, Lídia, Riancho, Javier, Tapia, Olga, Tarabal Mostazo, Olga   +7 more
core   +2 more sources

qRT-PCR versus IFA-based Quantification of Male and Female Gametocytes in Low-Density Plasmodium falciparum Infections and Their Relevance for Transmission

Journal of Infectious Diseases, 2019
Background Accurate quantification of female and male gametocytes and sex ratios in asymptomatic low-density malaria infections are important for assessing their transmission potential.
Maria Gruenberg, Natalie E Hofmann, E. Nate, S. Karl, L. Robinson, Kjerstin Lanke, Thomas A. Smith, T. Bousema, I. Felger   +8 more
semanticscholar   +1 more source

qRT-PCR (SYBR Green) v1

Quantitative reverse-transcription PCR (qRT-PCR) to measure relative gene expression using TRIzol RNA extraction, PrimeScript‱ RT Master Mix, and SYBR‱ Green on a 96-well real-time instrument (e.g., StepOnePlus‱). Analysis by 2^-ΔΔCT with melt-curve confirmation.
Armin Bayati, Yuhang Zhao
openaire   +1 more source

A Vascular Microphysiological Model of Lung Fibrosis Reveals That Myofibroblasts and IPF Patient‐Derived Fibroblasts Impair Angiogenesis and Vasculogenesis

Advanced Functional Materials, EarlyView.
A microphysiological lung fibrosis model recapitulates myofibroblast–vascular interactions. Induced myofibroblasts and patient‐derived IPF fibroblasts impair angiogenesis and increase vascular permeability via TGF‐β1–driven signaling. Pharmacological interventions with SB 431542 and VEGF supplementation restore vascular morphology and barrier function.
Elena Cambria, Adriana Blazeski, Eunkyung Clare Ko, Tran Thai, Shania Dantes, David A. Barbie, Sarah E. Shelton, Roger D. Kamm   +7 more
wiley   +1 more source