Results 161 to 170 of about 1,617,447 (322)

Evaluation of the effects of tropical tanniferous plants on rumen microbiota using qRT PCR and DGGE analysis

Czech Journal of Animal Science, 2013
Tanniferous forages may have bacteriostatic and/or bactericidal effect on different rumen microbial populations. We investigated the influence of the tropical tanniferous plants Styzolobium aterrimum (STA), Styzolobium deeringianum (STD), Leucaena ...
C. Longo, A.L. Abdalla, J. Liebich, I. Janzik, J. Hummel, P.S. Correa, K.-H. Südekum, P. Burauel   +7 more
doaj   +1 more source

Identification and evaluation of reference genes for qRT-PCR studies in Lentinula edodes

PLoS ONE, 2018
Lentinula edodes (shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. It contains various medically important molecules, such as polysaccharides, terpenoids, sterols, and lipids, were ...
Q. Xiang, Jin Li, P. Qin, Maolan He, Xiumei Yu, K. Zhao, Xiao-ping Zhang, Menggen Ma, Qiang Chen, Xiaoqiong Chen, Xianfu Zeng, Yun-fu Gu   +11 more
semanticscholar   +1 more source

Targeting Post‐Irradiation Thyroid Dysfunction: Electrospun Scaffolds As A Dual‐Action Approach for Antioxidant and Immune Modulation

Advanced Healthcare Materials, EarlyView.
Radiation‐induced hypothyroidism follows head and neck radiotherapy due to oxidative stress and inflammation. Electrospun polycaprolactone scaffolds containing adenosine have potential to modulate thyroid repair. Scaffolds enhance thyrocyte proliferation, antioxidant enzymes glutathione peroxidase and catalase, reduce senescence and apoptosis markers ...
Maria Heim, Ella‐Louise Handley, Daniel Grant, Lizi M. Hegarty, Elaine Emmerson, Anthony Callanan   +5 more
wiley   +1 more source

Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.

PLoS ONE, 2012
qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently,
Natalia V Demidenko, Aleksey A Penin
doaj   +1 more source

Profiling of Circulating Serum MicroRNAs in Children with Autism Spectrum Disorder using Stem-loop qRT-PCR Assay

Folia Medica, 2017
Background: Development of biomarkers for autism spectrum disorder (ASD) has still remained a challenge to date. Recently, alterations of the expression of microRNAs (miRNAs) in peripheral blood, serum and post-mortem brain tissue have been linked to ASD.
Tatyana M Kichukova, N. Popov, I. Ivanov, T. Vachev   +3 more
semanticscholar   +1 more source

Ascorbic Acid Modulates Collagen Properties in Glucocorticoid‐Induced Osteoporotic Bone: Insights into Chemical, Mechanical, and Biological Regulation

Advanced Healthcare Materials, EarlyView.
Osteoporosis from long‐term glucocorticoid (GIOP) use elevates susceptibility to fracture. This study shows GCs impair ascorbic acid (AA) metabolism in osteoblasts, collagen synthesis and extracellular matrix integrity. AA enhanced collagen biochemical and mechanical properties and restored osteoblast and endothelial function. These findings underscore
Micaila DE Curtis, Yoontae Kim, Rahul Patil, Xinran Zhang, Meth Jayatilake, Orelle Bulgin, Peter Lialios, Sana Surrency, Soraya Tarrah, Argyris K. Lazaris, Alison Grafton, James Williams IV, Seyedeh Boshra Nouraie, Maria Bizaki, Penelope Xeron, Edward Van Keuren, Jonathan E. Seppala, Evagelia C. Laiakis, Joanna B. Kitlinska, Stella Alimperti   +19 more
wiley   +1 more source

Quantitative RT-PCR assay for high-throughput screening (HTS) of drugs against the growth of Cryptosporidium parvum in vitro

Frontiers in Microbiology, 2015
Our laboratory has previously developed a qRT-PCR assay to assess drug efficacy on the growth of Cryptosporidium parvum in vitro by detecting the levels of parasite 18S rRNA.
Haili eZhang, Guan eZhu
doaj   +1 more source

Bioengineered Pancreatic Cancer Immunosuppressive Microenvironment Models for Screening Immunotherapies

Advanced Healthcare Materials, EarlyView.
Bioengineering tumor‐stroma pancreatic cancer models with potential to emulate the native immunosuppressive tumor microenvironment. Cancer‐on‐a‐bead models exhibited higher relevance for screening immunotherapies and modulating the protumoral PDAC microenvironment. ABSTRACT Pancreatic cancer is notably resistant to treatment, primarily due to its dense
Maria V. Monteiro, Margarida Henriques‐Pereira, Bruno M. Neves, Vítor M. Gaspar, João F. Mano   +4 more
wiley   +1 more source

Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling

Advanced Healthcare Materials, EarlyView.
A fully synthetic, compartmentalized 3D kidney disease model is introduced. The kidney model combines a PEG‐based hydrogel matrix with anisotropic, enzymatically degradable rod‐shaped microgels to spatially arrange a triple co‐culture of key renal epithelial, endothelial, and fibroblast cells.
Ninon Möhl, Daphne Bouwens, Johanna Abele, Aline Hans, Tanja Topic, Daniel Günther, Jitske Jansen, Rafael Kramann, Laura De Laporte   +8 more
wiley   +1 more source

Pathogen Response Genes Mediate Caenorhabditis elegans Innate Immunity

, 2012
Innate immunity is crucial in the response and defense against pathogens for invertebrates and vertebrates alike. The soil nematode Caenorhabditis elegans is a useful model to study the eukaryotic innate immune response to microbial pathogenesis.
Anthony, Hannah L.
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