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Gene Amplification and qRT-PCR
2014This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Cerith, Jones, Alain, Filloux
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Stem-Loop qRT-PCR–Based Quantification of miRNAs
2021Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR.
Yoann, Abel, Mathieu, Rederstorff
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RSV Growth and Quantification by Microtitration and qRT-PCR Assays
2016Defective interfering viral particles have been reported as important determinants of the course of viral infection, and they can markedly temper the virulence of the infection. Here, we describe a simple method, based on limiting dilution, for the removal of defective interfering particles from RSV.
Hayat, Caidi +2 more
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UNDERSTANDING qRT PCR: BASIC PRINCIPLES AND APPLICATIONS
2023The quantitative Reverse Transcription Polymerase Chain Reaction, abbreviated as qRT PCR is a major advancement of the Polymerase Chain Reaction (PCR) technology. Of all the methods available, qRT PCR is the most rapid, sensitive and accurate methodology for measurement of RNA.
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Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR
2015Whole-organ molecular analysis of the kidney potentially misses important factors involved in the pathogenesis of disease in glomeruli and tubules. Organ wide analysis can however be augmented by using laser capture microdissection (LCM) to isolate morphologically similar cells and nephron structures from a heterogeneous tissue section via direct ...
Tim D, Hewitson +2 more
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Quantification of NMDAR Subunit Genes Expression by qRT-PCR
2017Transcription is the initial and generally the most sensitive step to cellular needs and environmental cues. Thus, it serves as a major mechanism controlling gene expression. Using reverse-transcription quantitative polymerase chain reaction technology (RT-qPCR), we will present how to quantify the transcriptional expression of NMDARs subunits during ...
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Stem-Loop qRT-PCR for the Detection of Plant microRNAs
2016Plant microRNAs (miRNAs) play important roles in the posttranscriptional regulation of protein-coding genes, and they are essential for a normal development and survival. Mature miRNAs are cleaved from larger precursor RNAs and are typically 21-22 nt long.The small size, the lack of a common feature like a poly(A) tail, 3' end-modifications, and ...
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qRT-PCR’s Significance and Efficacy in Molecular Biology Research
International Journal on Emerging TechnologiesQuantitative Real-Time PCR (qRT-PCR) is one of the most in vitro innovative methods in molecular biology research for analyzing nucleic acids (RNA and DNA). It uses fluorescent reporter molecular techniques to track production of amplified products and analyze expression of specific genes (decipher the functional genes) through creation of cDNA from ...
null Ammar M. F. Mohammed +10 more
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Considerations and Suggestions for the Reliable Analysis of miRNA in Plasma Using qRT-PCR
Genes, 2022Eun Joo Song, Eunmi Ban
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