Results 161 to 170 of about 1,161,528 (204)

Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae). [PDF]

Insects
Yang G, Yu X, Zhang Y, Luo J, Li X, Zhu L, Zhang H, Jin L, Wu G, Yan X, Shen C.   +10 more
europepmc   +1 more source

Application of novel dark-quencher labeled probes in multiplex qRT-PCR assays for rapid detection of SARS-CoV-2 variants. [PDF]

J Thorac Dis
Zeng Z, Yang J, Dai J, Zou L, Lin Z, Liu Y, Guan W, Li F, Zheng K, Yuan S, Sun F, He F, Hong Y, Li H, Liu W, Men G, Zhang X, Lan Y, Deng X, Li L, Lin Y, Lai H, Qian P, Fan Q, Jiang M, Li J, Tang G, Mo Q, Deng X, Huang J, Deng X, Yang Z.   +31 more
europepmc   +1 more source

Identification and validation of qRT-PCR reference genes for analyzing grape infection with gray mold. [PDF]

BMC Genomics
Tan L, Lu L, Sun W, Zhang X, Liu Y, Xiang Y, Yan H.   +6 more
europepmc   +1 more source

A TaqMan-Based qRT-PCR Assay for Accurate Evaluation of the Oncogenic <i>TrkAIII</i> Splice Variant in Tumor cDNAs. [PDF]

Cancers (Basel)
Sbaffone M, Farina AR, Martelli I, Pontieri E, Guadagni S, Mackay AR, Cappabianca L, Zelli V.   +7 more
europepmc   +1 more source

Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris. [PDF]

J Vis Exp
Kirk MJ, Xu C, Paules J, Rothman JH.
europepmc   +1 more source

Validation of suitable reference microRNAs for qRT-PCR in Osmanthus fragrans under abiotic stress, hormone and metal ion treatments. [PDF]

Front Plant Sci
Zhang Y, Yan Q, Xia H, Yang J, Zeng X, Li Z, Cai X, Zou J, Chen H.   +8 more
europepmc   +1 more source

Selection and validation of reference genes for qRT-PCR normalization in dayflower (Commelina communis) based on the transcriptome profiling. [PDF]

BMC Plant Biol
Yang Y, Cao G, Tang L.
europepmc   +1 more source

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qRT-PCR of Microbial Biofilms

Cold Spring Harbor Protocols, 2008
INTRODUCTIONBacteria growing in biofilms often express a different subset of genes compared to the same strains growing planktonically. Quantitative reverse transcriptase real time PCR (qRT-PCR) can be used effectively to quantify the number of RNA transcripts of specific genes from bacteria growing in biofilms.
Ailyn C, Pérez-Osorio, Michael J, Franklin   +1 more
openaire   +2 more sources

RNA Integrity and the Effect on the Real-Time qRT-PCR Performance

Molecular Aspects of Medicine, 2006
The assessment of RNA integrity is a critical first step in obtaining meaningful gene expression data. Working with low-quality RNA may strongly compromise the experimental results of downstream applications which are often labour-intensive, time-consuming, and highly expensive. Using intact RNA is a key element for the successful application of modern
Michael W Pfaffl
exaly   +3 more sources

qRT-PCR of Small RNAs

2010
Plant small RNAs are a class of 19- to 25-nucleotide (nt) RNA molecules that are essential for genome stability, development and differentiation, disease, cellular communication, signaling, and adaptive responses to biotic and abiotic stress. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs).
Varkonyi-Gasic, Erika, Hellens, Roger
openaire   +3 more sources