Retinoid X receptor γ predicts the prognosis and is associated with immune infiltration in kidney renal clear cell carcinoma: a qRT-PCR, TCGA and in silico research. [PDF]
BMC UrolDong J, Fan L, Wu Q, Zheng Z.
europepmc +1 more source
Identification of an appropriate reference gene for normalization of qRT-PCR expression analyses in human breast cancer cell lines: application to L-arginine depletion studies. [PDF]
J Cancer Res Clin OncolRöglin A +3 more
europepmc +1 more source
Assessment of reference genes for qRT-PCR normalization to elucidate host response to African swine fever infection. [PDF]
Braz J MicrobiolRajkhowa S +8 more
europepmc +1 more source
Detection of porphyromonas gingivalis in oral potentially malignant disorders and oral squamous cell carcinoma using qRT-PCR: A comparative study. [PDF]
J Oral Maxillofac PatholSuganya G +5 more
europepmc +1 more source
Comparative Analysis of Gene Expression at Sea Level and High Altitude: A Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) Approach. [PDF]
CureusAlharthi SB +9 more
europepmc +1 more source
Related searches:
INTRODUCTIONBacteria growing in biofilms often express a different subset of genes compared to the same strains growing planktonically. Quantitative reverse transcriptase real time PCR (qRT-PCR) can be used effectively to quantify the number of RNA transcripts of specific genes from bacteria growing in biofilms.
Ailyn C, Pérez-Osorio +1 more
openaire +2 more sources
Stem-Loop qRT-PCR–Based Quantification of miRNAs
2021Stem-loop qRT-PCR is one of the most commonly used real-time PCR approach to quantify small non-coding RNAs such as microRNAs. The quantification method is divided in two steps. First, RNA is reverse transcribed using a specific stem-loop primer, and the resulting RT product is subsequently used as a template for quantitative real-time PCR.
Yoann, Abel, Mathieu, Rederstorff
openaire +2 more sources
Plant small RNAs are a class of 19- to 25-nucleotide (nt) RNA molecules that are essential for genome stability, development and differentiation, disease, cellular communication, signaling, and adaptive responses to biotic and abiotic stress. Small RNAs comprise two major RNA classes, short interfering RNAs (siRNAs) and microRNAs (miRNAs).
Varkonyi-Gasic, Erika, Hellens, Roger
openaire +3 more sources
Gene Amplification and qRT-PCR
2014This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell
Cerith, Jones, Alain, Filloux
openaire +2 more sources
Continuous-flow, microfluidic, qRT-PCR system for RNA virus detection
Analytical and Bioanalytical Chemistry, 2017One of the main challenges in the diagnosis of infectious diseases is the need for rapid and accurate detection of the causative pathogen in any setting. Rapid diagnosis is key to avoiding the spread of the disease, to allow proper clinical decisions to be made in terms of patient treatment, and to mitigate the rise of drug-resistant pathogens.
B Leticia, Fernández-Carballo +7 more
openaire +2 more sources