Results 21 to 30 of about 383,746 (345)

High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number

Analytical Chemistry, 2011
Digital PCR enables the absolute quantitation of nucleic acids in a sample. The lack of scalable and practical technologies for digital PCR implementation has hampered the widespread adoption of this inherently powerful technique. Here we describe a high-
B. Hindson, K. Ness, D. Masquelier, P. Belgrader, Nick Heredia, A. Makarewicz, Isaac J. Bright, M. Lucero, A. Hiddessen, T. Legler, Tyler K. Kitano, Michael R. Hodel, J. Petersen, Paul W Wyatt, Erin R. Steenblock, Pallavi H. Shah, L. Bousse, C. Troup, Jeff Mellen, D. Wittmann, Nicholas G. Erndt, T. Cauley, R. Koehler, A. So, S. Dube, K. Rose, Luz Montesclaros, Shenglong Wang, D. Stumbo, Shawn Hodges, S. Romine, F. Milanovich, H. White, John F. Regan, G. Karlin-Neumann, Christopher M. Hindson, Serge Saxonov, B. Colston   +37 more
semanticscholar   +1 more source

Evaluation of ViroCyt® Virus Counter for Rapid Filovirus Quantitation

Viruses, 2015
Development and evaluation of medical countermeasures for diagnostics, vaccines, and therapeutics requires production of standardized, reproducible, and well characterized virus preparations. For filoviruses this includes plaque assay for quantitation of
Cynthia A. Rossi, Brian J. Kearney, Scott P. Olschner, Priscilla L. Williams, Camenzind G. Robinson, Megan L. Heinrich, Ashley M. Zovanyi, Michael F. Ingram, David A. Norwood, Randal J. Schoepp   +9 more
doaj   +3 more sources

Insights into the Mechanism of Separation of Bisphosphonates by Zwitterionic Hydrophilic Interaction Liquid Chromatography: Application to the Quantitation of Risedronate in Pharmaceuticals

Separations, 2019
Bisphosphonates are used to treat various skeletal disorders, as they modulate bone metabolism by inhibition of the osteoclast-mediated bone resorption.
Irene Panderi, Eugenia Taxiarchi, Constantinos Pistos, Eleni Kalogria, Ariadni Vonaparti   +4 more
doaj   +1 more source

Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Journal of Immunology, 1972
A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after
E. Engvall, P. Perlmann
semanticscholar   +1 more source

MODERN STEREOLOGICAL EVALUATION IN THE AGING HUMAN SUBSTANTIA NIGRA

Image Analysis and Stereology, 2011
Quantitative estimation of neuronal numbers in the human substantia nigra (SN) can be achieved by a conventional single section (SS) count or by the more modern stereological disector (DS) count.
Shuang Y Ma, Frank M Longo, Matias Röyttä, Yrjö Collan   +3 more
doaj   +1 more source

Assessment of the direct quantitation of SARS-CoV-2 by droplet digital PCR

Scientific Reports, 2020
Droplet digital PCR (ddPCR) is a sensitive and reproducible technology widely used for quantitation of several viruses. The aim of this study was to evaluate the 2019-nCoV CDC ddPCR Triplex Probe Assay (BioRad) performance, comparing the direct ...
M. Deiana, A. Mori, C. Piubelli, Salvatore Scarso, M. Favarato, Elena Pomari   +5 more
semanticscholar   +1 more source

Quantitative testing [PDF]

Proceedings of the 8th ACM international conference on Embedded software, 2008
We investigate the problem of specification based testing with dense sets of inputs and outputs, in particular with imprecision as they might occur due to errors in measurements, numerical instability or noisy channels. Using quantitative transition systems to describe implementations and specifications, we introduce implementation relations that ...
Bohnenkamp, H.C., Stoelinga, Mariëlle Ida Antoinette   +1 more
openaire   +2 more sources

Anthocyanin Characterization, Total Phenolic Quantification and Antioxidant Features of Some Chilean Edible Berry Extracts

Molecules, 2014
The anthocyanin composition and HPLC fingerprints of six small berries endemic of the VIII region of Chile were investigated using high resolution mass analysis for the first time (HR-ToF-ESI-MS).
Anghel Brito, Carlos Areche, Beatriz Sepúlveda, Edward J. Kennelly, Mario J. Simirgiotis   +4 more
doaj   +1 more source

Rapid and ultra-sensitive quantitation of disease-associated α-synuclein seeds in brain and cerebrospinal fluid by αSyn RT-QuIC

Acta Neuropathologica Communications, 2018
The diagnosis and treatment of synucleinopathies such as Parkinson disease and dementia with Lewy bodies would be aided by the availability of assays for the pathogenic disease-associated forms of α-synuclein (αSynD) that are sufficiently sensitive ...
B. Groveman, C. Orrú, A. Hughson, L. Raymond, G. Zanusso, B. Ghetti, Katrina J Campbell, J. Safar, D. Galasko, B. Caughey   +9 more
semanticscholar   +1 more source

Pharmacokinetics and metabolism of ketoconazole after single ocular instillation in Sprague-Dawley rats

ADMET and DMPK
Background and purpose: Ketoconazole is limited to its conditioned oral use due to hepatic toxicity. Its ocular eye drop administration may be an option for mycotic keratitis treatment.
Jiang Pu, Jingsong He, Ru Xue, Ruiqi Gao, Yaoming Yu, Wanyong Feng   +5 more
doaj   +1 more source