Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley +1 more source
The Spread of COVID-19 Crisis Communication by German Public Authorities and Experts on Twitter: Quantitative Content Analysis. [PDF]
Drescher LS +5 more
europepmc +1 more source
Automation of the quantitative content analysis
The thesis is a summary of the state-of-the-art language technologies could be used for automation of the coding phase of the quantitative content analysis pipeline.
Libovický, Jindřich
core
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz +6 more
wiley +1 more source
Investigation of self-regulation skills in children's picturebooks: a quantitative content analysis. [PDF]
Gümrükçü Bilgici B.
europepmc +1 more source
Analysis of online parenting community posts on expanded newborn screening for metabolic disorders using topic modeling: a quantitative content analysis. [PDF]
Lee MS, Chung HS, Kim JS.
europepmc +1 more source
Quantitative analysis of technology futures. Part I: Techniques, contexts, and organizations
As a gentle introduction to quantitative foresight techniques we begin by providing a working definition of future-oriented technology analysis (FTA) and discussing its role, uses, and popularity over recent decades.
Rafols, Ismael +2 more
core
Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj +3 more
wiley +1 more source
Examining the news media reaction to a national sugary beverage tax in South Africa: a quantitative content analysis. [PDF]
Essman M +4 more
europepmc +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source

