Results 11 to 20 of about 228,684 (270)

Comparison of quantitative fluorescent polymerase chain reaction and karyotype analysis for prenatal screening of chromosomal aneuploidies in 270 amniotic fluid samples

Journal of Perinatal Medicine, 2019
Background Quantitative fluorescent polymerase chain reaction (QF-PCR) technique is a rapid prenatal aneuploidy detection method. This method can diagnose abnormality in chromosome 13, 18, 21, X and Y.
Nooshin Masoudzadeh, S. Teimourian
semanticscholar   +2 more sources

Quantitative fluorescent polymerase chain reaction for rapid prenatal diagnosis of fetal aneuploidies in chorionic villus sampling in a single institution. [PDF]

Obstet Gynecol Sci, 2016
Objective To validate quantitative fluorescent polymerase chain reaction (QF-PCR) via chorionic villus sampling (CVS) for the diagnosis of fetal aneuploidies.
Shin YJ, Chung JH, Kim DJ, Ryu HM, Kim MY, Han JY, Choi JS.   +6 more
europepmc   +2 more sources

Polymerase chain reaction combined with fluorescent lateral flow immunoassay based on magnetic purification for rapid detection of canine parvovirus 2

BMC Veterinary Research, 2019
BackgroundCanine parvovirus 2 (CPV-2) is one of the most common etiological agents that cause severe gastroenteritis in puppies. Early accurate diagnosis is important for infected dogs.
L. Zhuang, Yongxin Ji, Peilong Tian, Kaixuan Wang, C. Kou, N. Gu, Yu Zhang   +6 more
semanticscholar   +2 more sources

Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus

, 2018
Establishment of diagnostic methods with low detection limits plays a critical role in the maintenance of early diagnosis, prevention of serious neurological complications, and control of the spread of ZIKA. In this study, we established the micro-droplet digital polymerase chain reaction (ddPCR) and real-time fluorescent quantification PCR (qPCR ...
Yuan Hui, Zhiming Wu, Zhiran Qin, Li Zhu, Junhe Liang, Xujuan Li, Hanmin Fu, Shiyu Feng, Weiwei Xiao, Qinghua Wu, Bao Zhang, Wei Zhao   +11 more
openaire   +3 more sources

Her2 assessment using quantitative reverse transcriptase polymerase chain reaction reliably identifies Her2 overexpression without amplification in breast cancer cases

Journal of Translational Medicine, 2017
Immunohistochemistry (IHC) and fluorescent-in situ hybridization (FISH) are standard methods to assess human epidermal growth factor receptor 2 (HER2) status in breast cancer (BC) patients.
G. Zoppoli, A. Garuti, G. Cirmena, L. D. di Cantogno, C. Botta, M. Gallo, D. Ferraioli, E. Carminati, P. Baccini, M. Curto, P. Fregatti, E. Isnaldi, M. Lia, R. Murialdo, D. Friedman, A. Sapino, A. Ballestrero   +16 more
semanticscholar   +2 more sources

Instrumentation and Fluorescent Chemistries Used in Quantitative Polymerase Chain Reaction [PDF]

Polymerase Chain Reaction: Theory and Technology, 2019
Mathilde H. Josefsen, Charlotta Löfström, Trine Hansen, Eyjólfur Reynisson, Jeffrey Hoorfar   +4 more
openaire   +2 more sources

Rapid diagnosis of trisomy 18 of maternal origin by quantitative fluorescent polymerase chain reaction analysis following tissue culture failure for conventional cytogenetic analysis in a fetus with holoprosencephaly, ventricular septal defect, arthrogryposis of bilateral wrists and aplasia of the t

Taiwanese Journal of Obstetrics & Gynecology, 2021
OBJECTIVE We present rapid diagnosis of trisomy 18 of maternal origin by quantitative fluorescent polymerase chain reaction (QF-PCR) analysis following tissue culture failure for conventional cytogenetic analysis in a fetus with holoprosencephaly (HPE ...
Chih-ping Chen, Shih-Shien Weng, S. Chern, Shin-Wen Chen, Fang-Tzu Wu, Wayseen Wang   +5 more
semanticscholar   +1 more source

Quantitative Fluorescent Polymerase Chain Reaction for Prenatal Diagnosis and Its Application in Vietnam: A Literature Review [PDF]

, 2021
Early identification of fetal abnormalities is a huge challenge for modern obstetrics. Quantitative fluorescent polymerase chain reaction (QF-PCR) has quickly become an effective means of chromosome anomaly detection due to its advantages in terms of ...
Phan, Thuan
core   +1 more source

Effect of substrate thermal resistance on space-domain microchannel [PDF]

, 2009
In recent years, Fluorescent Melting Curve Analysis (FMCA) has become an almost ubiquitous feature of commercial quantitative PCR (qPCR) thermal cyclers. Here a micro-fluidic device is presented capable of performing FMCA within a microchannel.
Dalton, Tara, Kinahan, David J., Davies, Mark   +2 more
core   +1 more source

Noninvasive prenatal diagnosis of fetal RhD status using cell-free fetal DNA in maternal plasma with TaqMan® real-time PCR assay [PDF]

, 2007
Prenatal diagnosis is now part of established obstetric practice in many countries. However, conventional methods of prenatal diagnosis of obtaining fetal tissues for genetic analysis, including amniocentesis and chorionic villus sampling, are invasive
Rekhviashvili, Tea
core   +1 more source