Results 21 to 30 of about 228,684 (270)

Comparison of diagnostic methods for the detection and quantification of the four sympatric Plasmodium species in field samples from Papua New Guinea [PDF]

, 2010
Accurate diagnosis of Plasmodium infections is essential for malaria morbidity and mortality reduction in tropical areas. Despite great advantages of light microscopy (LM) for malaria diagnosis, its limited sensitivity is a critical shortfall for ...
Barnadas, Céline, Siba Peter, Betuela Inoni, Mueller Ivo, Zimmerman, Peter A., Rosanas-Urgell, Anna, Felger Ingrid, Felger, Ingrid, Rosanas-Urgell Anna, Dania Mueller, Mueller, Dania, Céline Barnadas, Ingrid Felger, Del Portillo, HA, Iga, Jonah, Felger, I, Hernando A del Portillo, Mueller, Ivo, Mueller Dania, Mueller, D, Anna Rosanas-Urgell, Siba, P, del Portillo, Hernando A., del Portillo Hernando A, Ivo Mueller, Iga Jonah, Peter Siba, Zimmerman, PA, Siba, Peter, Barnadas, C, Betuela, Inoni, Mueller, I, Barnadas Céline, Inoni Betuela, Jonah Iga, Betuela, I, Zimmerman Peter A, Peter A Zimmerman, Iga, J, Rosanas-Urgell, A   +39 more
core   +1 more source

Emerging Technologies for the Detection of Rabies Virus: Challenges and Hopes in the 21st Century [PDF]

, 2009
The diagnosis of rabies is routinely based on clinical and epidemiological information, especially when exposures are reported in rabies-endemic countries.
Müller, Thomas, Banyard, AC, Conrad M Freuling, Freuling, Conrad Martin, Dastjerdi, A., McElhinney, LM, Dastjerdi, A, Akbar Dastjerdi, Anthony R Fooks, Weiss, RA, Thomas Müller, Freuling, C.M., McElhinney, L.M., Edward Wright, Banyard, A.C., Lorraine M McElhinney, Fooks, AR, Johnson, N., Wright, E, Muller, T, Fooks, A.R., Wakeley, P.R., Wakeley, PR, Robin A Weiss, Philip R Wakeley, Johnson, N, Weiss, R.A., Marston, D.A., Muller, T., Marston, DA, Denise A Marston, Ashley C Banyard, Wright, E., Nicholas Johnson, Freuling, CM   +34 more
core   +1 more source

Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms [PDF]

, 2008
Background The real-time polymerase chain reaction is currently the method of choice for quantifying nucleic acids in different DNA based quantification applications. It is widely used also for detecting and quantifying genetically modified components in
Žel Jana, Cankar, K., Cankar Katarina, Buh Gašparič Meti, Buh Gašparič, Meti, Gasparic, M.B., Gruden Kristina, Gruden, Kristina, Cankar, Katarina, Gruden, K., Zel, J., Žel, Jana   +11 more
core   +1 more source

Real-time Polymerase Chain Reaction [PDF]

, 2020
: The real-time polymerase chain reaction (RT-PCR), also called quantitative real-time polymerase chain reaction (QRT-PCR) or kinetic polymerase chain reaction (kPCR), is a technique used to simultaneously quantify and amplify a DNA molecule.
Mei-Ying Chuang, Geroge Chen, Kuan-Jiunn Shieh, X Tracy Qiao, Hongbao Ma   +4 more
core  

Hematopoietic chimerism after allogeneic stem cell transplantation : a comparison of quantitative analysis by automated DNA sizing and fluorescent in situ hybridization

, 2005
Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is performed mainly in patients with high-risk or advanced hematologic malignancies and congenital or acquired aplastic anemias.
Wachowiak, Jacek, Tomasz Strabel, Peter Bader, Bader, Peter, Jólkowska, Justyna, Jacek Wachowiak, Michał Witt, Anna Pieczonka, Strabel, Tomasz, Pieczonka, Anna, Dariusz Boruczkowski, Boruczkowski, Dariusz, Justyna Jółkowska, Witt, Michal   +13 more
core   +1 more source

Accurate quantification of microorganisms in PCR-inhibiting environmental DNA extracts by a novel Internal Amplification Control approach using Biotrove OpenArrays

, 2009
PCR-based detection assays are prone to inhibition by substances present in environmental samples, thereby potentially leading to inaccurate target quantification or false-negative results.
Gent-Pelzer, M. van, Gent-Pelzer, M.P.E., van, Van Doorn, R., Doorn, R. van, Speksnijder, A., Speksnijder, A.G.C.L., Klerks, M.M., Schoen, C.D., van Gent-Pelzer, M., Klerks, M., Gent-Pelzer van, M., Doorn, R., van, Kowalchuk, G.A., van Gent-Pelzer, M.P.E.   +13 more
core   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Dimethyl fumarate combined with cisplatin at subcytotoxic doses sensitizes cervical cancer toward ferroptosis and apoptosis through GSH restriction and p53 (re)activation

Molecular Oncology, EarlyView.
Dimethyl fumarate (DMF) reduces growth of HPV‐positive cervical cancer spheroids and induces ferroptosis in cervical cancer cells via blocking SLC7A11/Glutathione (GSH) axis. Combination of subcytotoxic doses of DMF and cisplatin (CDDP) further suppresses spheroid growth and drives cell death in 2D culture models.
Carolina Punziano, Giuseppina Minopoli, Simona Romano, Laura Marrone, Katia Aquilano, Maria Lina Tornesello, Raffaella Faraonio   +6 more
wiley   +1 more source

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source