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Quantitative phase imaging: introduction

Journal of the Optical Society of America A
Quantitative phase imaging (QPI), propelled by advancements in digital holography and computational imaging, has revolutionized the ability to retrieve phase delays with high precision. Over the past two decades, the field has seen tremendous growth, contributing to numerous applications in biomedicine and material metrology, including live cell ...
Chenfei Hu   +5 more
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Quantitative phase imaging in biomedicine

Nature Photonics, 2018
Quantitative phase imaging (QPI) has emerged as a valuable method for investigating cells and tissues. QPI operates on unlabelled specimens and, as such, is complementary to established fluorescence microscopy, exhibiting lower phototoxicity and no photobleaching. As the images represent quantitative maps of optical path length delays introduced by the
YongKeun Park   +2 more
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Quantitative Phase Imaging in Microscopy

2009
Phase imaging can be used in a reflection or transmission geometry. In biology it has the advantage of being marker-free. It can measure physical shape, and refractive index variations, ideally in three dimensions (3D). In transmission, phase is related to optical path difference.
Colin JR Sheppard   +2 more
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Digital holography for quantitative phase-contrast imaging

Optics Letters, 1999
We present a new application of digital holography for phase-contrast imaging and optical metrology. This holographic imaging technique uses a CCD camera for recording of a digital Fresnel off-axis hologram and a numerical method for hologram reconstruction.
E, Cuche, F, Bevilacqua, C, Depeursinge
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Quantitative Phase Imaging by Evanescent Wave Microscopy

Frontiers in Optics 2017, 2017
Here we show the versatility of Digital Holography Microscopy for the development of innovative systems for quantitative phase imaging of Total Internal Reflection.
Mandracchia B, Paturzo M, Ferraro P
openaire   +2 more sources

Full-field quantitative phase imaging

SPIE Proceedings, 2008
Full-field quantitative phase imaging provides useful endogenous contrast in a variety of biological specimens where contrast from other natural sources is small and the use of exogenous materials is undesirable. While the concepts of interferometric microscopy are simple and have long been known, diffculties in implementation have prevented this ...
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Time Stretch Quantitative Phase Imaging

2017
Label-free cell analysis is essential to personalized genomics, cancer diagnostics, and drug development as it avoids adverse effects of staining reagents on cellular viability and cell signaling. However, currently available label-free cell assays mostly rely only on a single feature and lack sufficient differentiation.
Ata Mahjoubfar   +2 more
openaire   +1 more source

Quantitative Phase Imaging Using Hard X Rays

Physical Review Letters, 1996
The quantitative imaging of a phase object using 16 keV x rays is reported. The theoretical basis of the techniques is presented along with its implementation using a synchrotron x-ray source. We find that our phase image is in quantitative agreement with independent measurements of the object.
, Nugent   +4 more
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Quantitative phase imaging by optimized asymmetric illumination

Applied Optics, 2017
We have presented a simple approach for quantitative phase imaging by optimizing asymmetric illumination of a conventional microscope. With this illumination, the light intensity modulation accompanying refraction at the surface profile of phase objects occurs, and "phase-gradient information" can be derived by detecting it.
Yoshimasa, Suzuki   +3 more
openaire   +2 more sources

Coded aperture pair for quantitative phase imaging

Optics Letters, 2014
This Letter proposes a novel quantitative phase-imaging approach by optically encoding light fields into a complementary image pair followed by computational reconstruction. We demonstrate that the axial intensity derivative for phase recovery can be well estimated by a coded-aperture image pair without z axial scanning.
Jiamin, Wu   +4 more
openaire   +2 more sources

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