Results 81 to 90 of about 9,244,583 (282)

Synergistic perspectives—How single‐molecule biophysics complement biochemical understanding

FEBS Letters, EarlyView.
In this review, we discuss how ensemble biochemistry and single‐molecule approaches are complementary, outline commonly used single‐molecule techniques, and illustrate their relevance through two representative case studies: chromatin organization by SMC complexes and pathway choice during DNA double‐strand break repair.
Sara De Bragança, Eline L. Jansen, Jorine M. Eeftens   +2 more
wiley   +1 more source

Phase shifting white light interferometry using colour CCD for optical metrology and bio-imaging applications

, 2018
Phase shifting white light interferometry (PSWLI) has been widely used for optical metrology applications because of their precision, reliability, and versatility.
Upputuri, Paul Kumar, Manojit Pramanik, Paul Kumar Upputuri, Pramanik, Manojit   +3 more
core   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Illumination coherence engineering and quantitative phase imaging [PDF]

Optics Letters, 2014
Partially coherent illumination provides significant advantages such as speckle-free imaging and enhanced optical sectioning in optical microscopy. The knowledge of the spatial and temporal coherence is crucial to obtain accurate quantitative phase imaging (QPI) of specimens such as live cells, micrometer-sized particles, etc.
Rodrigo Martín-Romo, José Augusto, Alieva Krasheninnikova, Tatiana   +1 more
openaire   +3 more sources

Tomographic microscope for low-coherent quantitative phase imaging

A novel tomographic microscope setup utilizing the principle of Holographic Incoherent-light-source Quantitative Phase Imaging is introduced. This setup combines the advantages of achromatic off-axis holography with the ultrafast operation of a digital ...
Buček, Jozef, Špaček, Matěj, Pachl, Přemysl, Dvořák, Vladislav, Dostál, Zbyněk, Neumanová, Anna   +5 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Effect of tissue staining in quantitative phase imaging

Journal of Biophotonics, 2018
Quantitative phase imaging (QPI) is an emerging modality, which enables the identification of abnormalities in tissue based on optical properties. QPI can be applied to any biological specimen due to its label‐free imaging capability, but its use in stained tissue is unclear. Here, we study the variability of QPI with the staining dye.
Sungbea Ban, Eunjung Min, Yujin Ahn, Gabriel Popescu, Woonggyu Jung   +4 more
openaire   +4 more sources

Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein

FEBS Letters, EarlyView.
Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux, Christian Lévêque, Fodil Azzaz, Marion Sangiardi, Sarosh R Irani, Michael Seagar, Jacques Fantini, Oussama El Far   +7 more
wiley   +1 more source

High-throughput transport-of-intensity quantitative phase imaging with aberration correction

Light: Advanced Manufacturing
The transport of intensity equation (TIE) is a well-established phase retrieval technique that enables incoherent diffraction limit-resolution imaging and is compatible with widely available brightfield microscopy hardware.
Linpeng Lu, Shun Zhou, Yefeng Shu, Yanbo Jin, Jiasong Sun, Ran Ye, Maciej Trusiak, Peng Gao, Chao Zuo   +8 more
doaj   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source