Results 191 to 200 of about 1,079,378 (256)

Analysis of relative genes expression and mutation of pstB and efpA efflux pumps in Mycobacterium simiae isolates from suspected tuberculosis patients by using quantitative Real-time PCR. [PDF]

BMC Microbiol
Hashemzadeh M, Hasanvand M, Montazeri EA.   +2 more
europepmc   +1 more source

Mechanically Spatio‐Chimeric Fibrin Assembly Enables Vascular‐Integrated Muscle Reconstruction for Volumetric Muscle Loss Repair

Advanced Materials, EarlyView.
SPARC (spatio‐chimeric, plasma‐based, anisotropic, and shear‐responsive construct) that integrates myogenic and vascular microenvironments within a single construct. The dual‐modulus matrix directs aligned myotube formation and endothelial network development, enabling a vascularized muscle implant that seamlessly anastomoses with host tissue and ...
Su Hyun Jung, Minjun Kim, Da‐Yoon Kim, Min Kyu Kim, Sieun Lee, Yoonhee Jin, Joo H. Kang   +6 more
wiley   +1 more source

Development and evaluation of a rapid diagnostic method for Sporothrix globosa in Asia using quantitative real-time PCR. [PDF]

BMC Infect Dis
Geng H, Sun L, Wang C, Zhang Y, Wang S, Cheng Y, Bao F, Liu H, Zhang F.   +8 more
europepmc   +1 more source

Immune and Stem Cell‐Mediated Bactericidal Amplification and Bone Remodeling for Infection Clearance and Osteointegration

Advanced Materials, EarlyView.
A pH‐responsive biomaterial interface integrates bactericidal peptides, cell‐recruiting peptides, and magnesium ions into a multifunctional system. Infection‐induced acidification triggers antimicrobial peptide release while cell‐recruiting peptides mobilize bone marrow stem cells.
Zhenyu Li, Siming Zhang, Jiale Dong, Ning Li, Mo Chen, Kunzheng Wang, Bin Li, Guoqing Pan, Jiaxiang Bai, Chen Zhu   +9 more
wiley   +1 more source

Selection and Validation of Reference Genes for Quantitative Real-Time PCR Analysis in Cockroach Parasitoid Tetrastichus hagenowii (Ratzeburg). [PDF]

Insects
Dong R, Cao F, Yu J, Yuan Y, Wang J, Li Z, Zhu C, Li S, Li N.   +8 more
europepmc   +1 more source

Development of Nanoflare Nanosensors for Monitoring Cellular Insulin mRNA in Beta Cells and Islets

Advanced Materials Technologies, EarlyView.
This study presents the design and application of In2‐NF probes for detecting insulin mRNA in beta cells and islets. Using spherical nucleic acid‐coated gold nanoparticles, the probes offer strong fluorescent signals upon hybridization. Their performance is validated across 2D and 3D cultures and rat islets, demonstrating sensitive, real‐time mRNA ...
Yana Hu, Qizheng Zhang, Tianli Hu, Juewen Liu, Hongxing Fu, Xin Zhao, Zijiang Yang, Chenjie Xu   +7 more
wiley   +1 more source

A Novel Normalized Quantitative Real-Time PCR Approach for Ensuring Roe Deer (Capreolus capreolus) Meat Authenticity in Game Meat Foods. [PDF]

Foods
Adenuga BM, Biltes R, Villa C, Costa J, Spychaj A, Montowska M, Mafra I.   +6 more
europepmc   +1 more source

Microfluidic Hydroporation Platform for Effective Allogeneic CAR‐T Cell Production and Subsequent Functional Cytotoxicity Analysis

Advanced Materials Technologies, EarlyView.
The hydroporator platform employs controlled hydrodynamic deformation for efficient mRNA and CRISPR/Cas9 delivery into primary human T cells, enabling allogeneic CAR‐T cell manufacturing. It preserves cell functionality and drives potent gene editing, CAR expression, and tumor cytotoxicity, while feature‐based analysis links these functional outcomes ...
Soohyun Jeon, Sein Kim, Hyun‐Young Kim, Duck Cho, Sungsu Park, Seohyun Kim, Aram J. Chung   +6 more
wiley   +1 more source

Selection of reference genes for quantitative real-time PCR analysis in exogenous hormone-treated <i>Lycoris aurea</i>. [PDF]

Acta Biochim Biophys Sin (Shanghai)
Zhao W, Tian Y, Wang Y, Wu Y, Ren Z, Li K.   +5 more
europepmc   +1 more source

Comparison of high and low virulence serotypes of Actinobacillus pleuropneumoniae by quantitative real-time PCR [PDF]

, 2010
Angen, Øystein, Boye, Mette, Schou, Kirstine Klitgaard   +2 more
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