Results 211 to 220 of about 1,080,483 (256)

The Uppsala APP Mutation Promotes Wild‐Type Amyloid‐β Aggregation and Deposition In Vivo

Advanced Science, EarlyView.
We investigated in vivo cross‐seeding of amyloid‐β (Aβ) isoforms in transgenic mice co‐expressing wild‐typeAβ and the Uppsala‐mutant Aβ variant (AβUpp), lacking six central residues. Weleveraged MALDI‐MS imaging and hyperspectral microscopy to follow spatio‐temporalAβ deposition.
Junyue Ge, María Pagnon de la Vega, Silvia Zampar, Enrica Cerilli, Srinivas Koutarapu, Ling Wu, Paul Fraser, Sertan Arkan, Vilmantas Giedraitis, Lars Lannfelt, Greta Hultqvist, Stina Syvänen, Martin Ingelsson, Jörg Hanrieder, Dag Sehlin   +14 more
wiley   +1 more source

Development of a biomarker-based platform for comprehensive skin characterization using minimally invasive skin sampling and quantitative real-time PCR. [PDF]

Skin Res Technol
Kim SH, Kim JH, Choi YM, Seo SM, Jang EY, Lee SJ, Zhang HS, Roh Y, Jung YW, Park CO, Jeong DH, Lee KH.   +11 more
europepmc   +1 more source

A Central Somatic Transmission Mediates Proprioceptive Facilitation of Muscle Pain

Advanced Science, EarlyView.
Zhang et al. uncover a novel central mechanism for persistent muscle pain, in which TRPA1 sensitization in MeV proprioceptive neurons enhances somatic secretion. This, in turn, disinhibits descending pain control from neighboring noradrenergic locus coeruleus neurons via local GABAergic circuits, thereby promoting inflammatory muscle pain.
Xiaoyu Zhang, Jiale Yang, Xi Wu, Jie Li, Fujian Lu, Yiman Li, Xingyu Du, Rong Huang, Jamila Asgar, Jing Wang, Ke Ren, Feipeng Zhu, Changhe Wang, Yehua Gan, Feng Wei, Zhuan Zhou   +15 more
wiley   +1 more source

Validation of a simplified small-scale DNA extraction protocol from wine by quantitative real-time PCR. [PDF]

3 Biotech
Scali M, Spinsanti G, Vignani R.
europepmc   +1 more source

Conformable Microelectrode Arrays Integrated with a Scoop‐Shaped Slide‐Well for Dynamic Electrophysiological Profiling of Patient‐Derived Cardiac Organoids

Advanced Science, EarlyView.
A flexible, ultrathin multi‐channel microelectrode array (MEA) conformally integrated onto a curved slide‐well enables stable electrical interfacing with 3D cardiac organoids. The geometry‐guided self‐alignment allows simultaneous recording of extracellular field potentials and contractile motion from irregular, beating tissues.
Ye Seul Kim, Jeonghwa Jeong, Gyeonghwa Heo, Young Woo Kwon, Jeongha Lee, Yoon Ji Jeong, Gyu Tae Park, Seongdo Jeong, Ki Won Hwang, Murim Choi, Jinhong Shin, Suck Won Hong, Jae Ho Kim   +12 more
wiley   +1 more source

Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues of Apocynum venetum. [PDF]

Genes (Basel)
Li X, Zhang T, Jiang L, Fan G.
europepmc   +1 more source

Evaluating the Utilities of Foundation Models in Single‐Cell Data Analysis

Advanced Science, EarlyView.
This study delivers the first systematic, task‐level evaluation of single‐cell foundation models across eight core analytical tasks. By benchmarking 10 leading models with the scEval framework, it reveals where foundation models truly add value, where task‐specific methods still dominate, and provides concrete, reproducible guidelines to steer the next
Tianyu Liu, Kexing Li, Yuge Wang, Hongyu Li, Hongyu Zhao   +4 more
wiley   +1 more source

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

Significant difference in gut microbiota Bifidobacterium species but not Lactobacillus species in colorectal cancer patients in comparison with healthy volunteers using quantitative real-time PCR. [PDF]

PLoS One
Esfandiari F, Bakhshi B, Shahbazi T, Derakhshan-Nezhad E, Bahroudi M, Minaeeian S, Boustanshenas M, Alborzi F, Behboudi B, Fazeli MS.   +9 more
europepmc   +1 more source

Zinc Exposure Causes Disulfidptosis to Induce Miscarriage by Up‐Regulating GATA1/METTL1/SLC7A11 Axis

Advanced Science, EarlyView.
Zn exposure up‐regulates GATA1, promoting GATA1‐mediated METTL1 and SLC7A11 transcription. It also enhances METTL1‐mediated m7G modification on SLC7A11 mRNA, increasing SLC7A11 mRNA stability. Ultimately, Zn exposure up‐regulates SLC7A11 at both transcriptional and post‐transcriptional levels, causing disulfidptosis. Knockdown of murine Slc7a11, Gata1,
Wenxin Huang, Yi Sun, Yanxin Wang, Haijun Yan, Xiaoping Yue, Weidong Wu, Xueyu Chen, Jinfang Zhang, Yanbing Lin, Qiong Lei, Nan Ji, Shuaishuai Xing, Liqin Zeng, Qingzheng Kang, Depeng Zhao, Geng Guo, Huidong Zhang   +16 more
wiley   +1 more source