Results 61 to 70 of about 1,079,378 (256)

Reference gene screening of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans for quantitative real-time PCR studies [PDF]

, 2019
Real-time quantitative PCR studies largely depend on reference genes for the normalization of gene expression. Stable reference genes should be accurately selected in order to obtain reliable results.
Martel, An, Pasmans, Frank, Verbrugghe, Elin   +2 more
core   +1 more source

RIPK4 function interferes with melanoma cell adhesion and metastasis

Molecular Oncology, EarlyView.
RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski, Sławomir Lasota, Ewelina Madej, Anna A. Brożyna, Małgorzata Szczygieł, Agnieszka Harazin‐Lechowska, Jan Czerbniak, Janusz Rys, Jaroslaw Czyz, Agnieszka Wolnicka‐Glubisz   +9 more
wiley   +1 more source

Application of droplet digital PCR for quantitative detection of Spiroplasma citri in comparison with real time PCR.

PLoS ONE, 2017
Droplet digital polymerase chain reaction (ddPCR) is a method for performing digital PCR that is based on water-oil emulsion droplet technology. It is a unique approach to measure the absolute copy number of nucleic acid targets without the need of ...
Yogita Maheshwari, Vijayanandraj Selvaraj, Subhas Hajeri, Raymond Yokomi   +3 more
doaj   +1 more source

Quantitative analysis of diet structure by real-time PCR, reveals different feeding patterns by two dominant grasshopper species [PDF]

, 2016
This study was supported by the National Natural Science Foundation of China, 31471823, the Earmarked Fund for China Agriculture Research System, CARS-35-07, and the Innovation Project of Chinese Academy of Agricultural Science.Studies on grasshopper ...
Cao, Guangchun, Huang, Xunbing, Ma, Jingchuan, McNeill, Mark Richard, Nong, Xiangqun, Qin, Xinghu, Tu, Xiongbing, Wang, Guangjun, Wu, Huihui, Zhang, Zehua   +9 more
core   +2 more sources

COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells

Molecular Oncology, EarlyView.
This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos, Gilar Gorji‐bahri, Lejla Gradjan, Julia Zajac, Kacper Gil, Yvonne Thokozile Nduku, Anna M. Blom   +6 more
wiley   +1 more source

Quantification of Intestinal Lactobacillus Species in Children with Functional Constipation by Quantitative Real-Time PCR

Clinical and Experimental Gastroenterology, 2020
Nabi Jomehzadeh,1– 3 Hazhir Javaherizadeh,4 Mansour Amin,2,3 Mohammad Rashno,1 Ali Teimoori5 1Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Infectious and Tropical Diseases Research Center ...
Jomehzadeh N, Javaherizadeh H, Amin M, Rashno M, Teimoori A   +4 more
doaj  

Validation of reference genes for gene expression studies in tartary buckwheat (Fagopyrum tataricum Gaertn.) using quantitative real-time PCR [PDF]

PeerJ, 2019
Quantitative real-time reverse transcriptase polymerase chain reaction is a sensitive technique for quantifying gene expression levels. By implementing three distinct algorithms (geNorm, normFinder and BestKeeper), we have validated the stability of the ...
Chenglei Li, Haixia Zhao, Maofei Li, Panfeng Yao, Qingqing Li, Xuerong Zhao, Anhu Wang, Hui Chen, Zizhong Tang, Tongliang Bu, Qi Wu   +10 more
doaj   +2 more sources

Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes [PDF]

, 2008
Background Recombinant protein production in Escherichia coli cells is a complex process, where among other parameters, plasmid copy number, structural and segregational stability of plasmid have an important impact on the success of productivity. It was
Mihaela Skulj, Veronika Okrslar, Spela Jalen, Simona Jevsevar, Petra Slanc, Borut Strukelj, Viktor Menart   +6 more
core   +2 more sources

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source

Cloning and expression of two new prolactin-related proteins, prolactin-related protein-VIII and -IX, in bovine placenta [PDF]

, 2005
Background Prolactin-related proteins (PRPs) are specific proteins of the growth hormone/prolactin (GH/PRL) family in bovine placenta. This study reports the identification and sequencing of a full-length cDNA for two new members of bovine PRPs, bPRP ...
Kaneyama Kanako, Hosoe Misa, Takahashi Toru, Ushizawa Koichi, Hashizume Kazuyoshi   +4 more
core   +1 more source