Results 151 to 160 of about 21,194,222 (201)

Quantitative detection of periodontopathogens by real-time PCR

Journal of Microbiological Methods, 2004
Specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. We report here the development of a sensitive, quantitative PCR technique for enumerating Actinobacillus actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg ...
Claudia, Nonnenmacher, Alexander, Dalpke, Reinier, Mutters, Klaus, Heeg   +3 more
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Accuracy of quantitative real-time PCR analysis

Doklady Biochemistry and Biophysics, 2013
When solving many problems in biology and med icine, it is necessary to detect the presence of specific nucleic acid sequences in samples. For this purpose, polymerase chain reaction (PCR)—a chain enzy matic reaction of multiplication of the analyzed DNA fragment, realized in the form of successive rounds of duplication of fragments present in the ...
D G, Sochivko, A A, Fedorov, D A, Varlamov, V E, Kurochkin, R V, Petrov   +4 more
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Real-Time Quantitative PCR of Telomere Length

Molecular Biotechnology, 2004
Telomeres cap the ends of chromosomes and are essential for the protection of chromosomes, as well as restricting the replicative potential of a cell. These functions are achieved by the regulation of telomeric repeat length, making the measurement of telomere length a useful aid in the elucidation of the replicative history and potential of cells ...
Marcel E, Gil, Thérèsa L, Coetzer
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Evaluation of uncertainty in quantitative real-time PCR

Journal of Microbiological Methods, 2006
Quantitative real-time PCR is one of the newer methods for measurement of the amount of nucleic material in biological systems. However, reliable measurement requires an appropriate estimation of uncertainty and this paper has developed the uncertainty budget associated with this procedure using as an example, data from a quantitative real-time PCR ...
John L, Love, Paula, Scholes, Brent, Gilpin, Marion, Savill, Susan, Lin, Laly, Samuel   +5 more
openaire   +2 more sources

Quantitative Real-Time PCR: Recent Advances

2016
Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a "closed-tube" system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or ...
Charanjeet, Singh, Sinchita, Roy-Chowdhuri   +1 more
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High‐Throughput Quantitative Real‐Time PCR

Current Protocols in Human Genetics, 2008
AbstractRecent technical advances in quantitative real‐time PCR (qRT‐PCR) have allowed for extensive miniaturization, thereby rendering the technique amenable to high‐throughput assays. Large numbers of different nucleic acids can now rapidly be measured quantitatively. Many investigations can benefit from this approach, including determination of gene
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Quantitative real-time RT-PCR assay for PCA3

Clinical Biochemistry, 2008
To develop a quantitative, internally standardized real-time RT-PCR assay for prostate cancer antigen 3 (PCA3), a non-translated gene found to be prostate-specific and highly overexpressed in cancer, and examine the role of PCA3 in peripheral blood with a small sample cohort.The RT-PCR assay for PCA3 is based on target-specific lanthanide probes ...
Riina-Minna, Väänänen, Maria, Rissanen, Otto, Kauko, Siina, Junnila, Ville, Väisänen, Jussi, Nurmi, Kalle, Alanen, Martti, Nurmi, Kim, Pettersson   +8 more
openaire   +2 more sources

Quantitative Real-Time PCR in aDNA Research

2011
Quantitative real-time PCR (qPCR) is a technique that is widely used in the field of ancient DNA (aDNA). Quantitative PCR can be used to optimize aDNA extraction methodologies, to detect PCR inhibition, and to quantify aDNA libraries for use in high-throughput sequencing.
Bunce, Michael, Oskam, C., Allentoft, M.
openaire   +3 more sources

Quantitative Real-Time RT-PCR

2011
Quantitative real-time PCR is a sensitive tool for quantitative expression analysis of RNA isolated from clinical samples and is increasingly being utilized in novel clinical diagnostic assays. It is an essential tool for the detection of degraded RNA, as that extracted from formalin-fixed paraffin-embedded (FFPE) tissues.
DOTTI, ISABELLA, NARDON, ERMANNO, PRACELLA, DANAE, BONIN, Serena   +3 more
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Accurate Real-time Reverse Transcription Quantitative PCR

2009
Within the last few years real-time quantitative PCR has become the method of choice for the accurate quantification of mRNA levels. Compared to previous methods the sensitivity of real-time quantitative PCR improved to the detection limit of up to one single molecule per reaction tube.
Marco, Klatte, Petra, Bauer
openaire   +2 more sources