Results 161 to 170 of about 21,194,222 (201)

Validation of a quantitative method for real time PCR kinetics

Biochemical and Biophysical Research Communications, 2002
Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which the quantitative methods are based. The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same amplicon ...
Liu, W., Saint, D.
openaire   +3 more sources

Detection of pathogenic leptospires by real-time quantitative PCR

Journal of Medical Microbiology, 2005
Definitive diagnosis of leptospirosis has traditionally depended upon the isolation of leptospires from clinical specimens or the demonstration of seroconversion in paired acute and convalescent serum samples. Both of these approaches require expertise not routinely available in clinical laboratories and usually result in delayed diagnosis ...
Paul N, Levett, Roger E, Morey, Renee L, Galloway, Danielle E, Turner, Arnold G, Steigerwalt, Leonard W, Mayer   +5 more
openaire   +2 more sources

New FRET primers for quantitative real-time PCR

Analytical and Bioanalytical Chemistry, 2007
FRET primer real-time PCR chemistry depends on internally labeled primers with FRET dyes linked to their 3' end. The best distance between the FRET dyes for obtaining the largest signal and the lowest background is six nucleotides. In this study the forward primer was labeled with FAM and the reverse primer with Texas red; the labeled primers meet in ...
Ashraf I, Ahmad, Jahan B, Ghasemi
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Real-Time Quantitative PCR for the Detection of Fetal Aneuploidies

2008
In prenatal analysis, one of the major concerns is the detection of fetal aneuploidies. Several molecular methods have been described recently for the rapid analysis of amniotic fluid and chorionic villi. Fluorescence in situ hybridization (FISH) and polymerase chain reaction (PCR) of short tandem repeats are already implemented in prenatal ...
Bernhard G, Zimmermann, Lech, Dudarewicz
openaire   +2 more sources

Diagnosis of aerobic vaginitis by quantitative real-time PCR

Archives of Gynecology and Obstetrics, 2016
To evaluate a real-time PCR-based technique to quantify bacteria associated with aerobic vaginitis (AV) as a potential test.Vaginal samples from 100 women were tested by wet-mount microscopy, gram stain and quantitative real-time PCR targeting Enterobacteriacea, Staphylococcus spp., Streptococcus spp., Enterococcus spp., Escherichia coli, Streptococcus
Rumyantseva, T. A., Bellen, G., Savochkina, Y. A., Guschin, A. E., Donders, G.G.G.   +4 more
openaire   +3 more sources

Real-Time Quantitative PCR, Pathogen Detection and MIQE

2012
Nucleic acids are the ultimate biomarker and real-time PCR (qPCR) is firmly established as the method of choice for nucleic acid detection. Together, they allow the accurate, sensitive and specific identification of pathogens, and the use of qPCR has become routine in diagnostic laboratories. The reliability of qPCR-based assays relies on a combination
Gemma, Johnson, Tania, Nolan, Stephen A, Bustin   +2 more
openaire   +2 more sources

RNA Isolation and Real-Time Quantitative RT-PCR

2008
Adipose tissue has emerged as a major endocrine organ producing a wide spectrum of hormones and factors that play crucial roles in regulating cell turnover and function, not only locally within the adipose tissue but also in the brain and other key metabolic organ systems.
Haiyan, Guan, Kaiping, Yang
openaire   +2 more sources

Quantitative (Real-Time) RT-PCR in Cardiovascular Research

2007
Quantitative (real-time) PCR (qPCR) represents a highly sensitive, sequence-specific, and reproducible technique for the gel-free detection and quantitation of nucleic acids. Owing to its large dynamic range and throughput, this approach has become the chosen method for rapid quantification of mRNA levels in biological samples.
Ashton, KJ, Headrick, JP
openaire   +2 more sources

Semi-Quantitative Real-Time PCR for Pain Research

2004
In this chapter we cover what we have found to be a "best practice" for real-time polymerase chain reaction (PCR) for relative mRNA quantification. We describe our techniques for tissue-sample collection and freezing, sample handling for quick and reproducible extraction of total RNA, first strand cDNA synthesis, real-time PCR amplification, and ...
Hong Qing, Guo, Sandra R, Chaplan
openaire   +2 more sources

Real-Time Quantitative PCR Analysis of Viral Transcription

2004
Whole-genome profiling using DNA arrays has led to tremendous advances in our understanding of cell biology. It has had similar success when applied to large viral genomes, such as the herpesviruses. Unfortunately, most DNA arrays still require specialized and expensive resources and, generally, large amounts of input RNA. An alternative approach is to
James, Papin, Wolfgang, Vahrson, Rebecca, Hines-Boykin, Dirk P, Dittmer   +3 more
openaire   +2 more sources