Results 11 to 20 of about 22,884 (241)
Quencher-free multiplexed monitoring of DNA reaction circuits. [PDF]
Nucleic Acids Res, 2012 We present a simple yet efficient technique to monitor the dynamics of DNA-based reaction circuits. This technique relies on the labeling of DNA oligonucleotides with a single fluorescent modification. In this quencher-free setup, the signal is modulated by the interaction of the 3'-terminus fluorophore with the nucleobases themselves. Depending on the
Padirac A, Fujii T, Rondelez Y.
europepmc +3 more sources
Quencher-free CRISPR-based molecular detection using an amphiphilic DNA fluorescence probe. [PDF]
Biosens BioelectronRapid, sensitive, and specific nucleic acid detection methods play crucial roles in clinical diagnostics and healthcare. Here, we report a novel amphiphilic DNA fluorescence probe for CRISPR-based nucleic acid detection. Unlike conventional fluorophore-quencher probe detection system, our amphiphilic DNA fluorescence probe features a hydrophobic Cy5 ...
Yang R +10 more
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Bio-Protocol, 2015 The ribonuclease H (RNase H) polymerase-independent cleavage assay allows detection and quantification of RNase H activity of reverse transcriptase (RT) enzymes with a hybrid substrate formed by a fluorescein labeled RNA annealed with Dabcyl DNA (Figure ...
Angela Corona, Enzo Tramontano
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Design of an ultimate quencher free molecular beacon containing pyrrolocytidine-guanine base pair [PDF]
Nucleic Acids Symposium Series, 2008 A novel quencher free molecular beacon was designed in which fluorophore-labelled pyrrolocytidine was placed away from the stem terminal. This new type of MB was used for the detection of a target DNA with an excellent efficiency.
Yoshio, Saito +5 more
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One-Step Assembly of Fluorescence-Based Cyanide Sensors from Inexpensive, Off-The-Shelf Materials
Sensors, 2020 We report a simple and versatile approach to assemble sensitive and selective fluorescence “turn-on” sensors for cyanide by combining three off-the-shelf materials; namely fluorescent dye, 1-vinyl imidazole polymer, and cupric chloride.
Gregory E. Fernandes +3 more
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Journal of Photochemistry and Photobiology, 2021 Bis-ethylhexyloxyphenol Methoxyphenyl Triazine (BEMT) was previously shown to efficiently quench the excited state of Butyl Methoxydibenzoylmethane (BMDBM) and to prevent its photodegradation.
Myriam Sohn +4 more
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Quencher-free linear probe with multiple fluorophores on an acyclic scaffold
Chemical Science, 2012 We have developed a new quencher-free stemless linear probe involving multiple perylenes incorporated through D-threoninol; each perylene is separated by intervening natural nucleotides. Without a substrate, the flexible linear probe does not emit fluorescence due to the self-quenching of the weakly interacting fluorophores. Upon hybridization with the
Hiroyuki Asanuma +5 more
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Aptamer-Based Fluorometric Ochratoxin A Assay Based on Photoinduced Electron Transfer
Toxins, 2019 This study describes a novel quencher-free fluorescent method for ochratoxin A (OTA) detection based on the photoinduced electron transfer (PIET) between guanine and fluorophore.
Han Zhao +3 more
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Molecules, 2022 Aflatoxin B1 (AFB1) is one of the mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus, and it causes contamination in foods and great risk to human health. Simple sensitive detection of AFB1 is important and demanded for food safety and
Chao Wang, Hao Yu, Qiang Zhao
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Biosensors, 2022 In this work, carbon dots (CDs) were synthesized by a one-step hydrothermal method using citric acid and ethylene diamine, and covalently functionalized with antibodies for the sensing of progesterone hormone.
Disha +4 more
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