Results 31 to 40 of about 22,884 (241)
Optimization of Graphene Oxide-based Quencher-free Molecular Beacon for Meat Product Authentication
Pakistan Journal of Biological Sciences, 2019 Sensitivity is very important in DNA detection. Various attempts have been made to increase detection sensitivity, including increasing the detection capabilities of devices and using DNA probes. This study was aimed to develop a DNA detection method using a quencher-free molecular beacon (QFMB) probe with the help of graphene oxide (GO) as a quencher ...
Hari, Widada +3 more
openaire +2 more sources
Materials & Design, 2022 Multiplexed detection is essential in biomedical sciences since it is more efficient and accurate than single-analyte detection. For an accurate early diagnosis of COVID-19, a multiplexed detection strategy is required to avoid false negatives with the ...
Menglin Song +8 more
doaj +1 more source
Probing the stable G-quadruplex transition using quencher-free end-stacking ethynyl pyrene–adenosine [PDF]
Chem. Commun., 2007 Pyrene-modified adenosines in the dangling positions of G-rich oligodeoxynucleotides undergo pi-stacking in their G-quadruplex formation, but not in their single strands, which can be characterized by fluorescence lambda(max) changes that occur on stacking.
Seo, YJ, Lee, IJ, Yi, JW, Kim, BH
openaire +3 more sources
Plants, 2023 Considering the great potential of black soybean seed coat as a source of bioactive compounds, the objective of this study was to investigate the effect of anthocyanin-rich brine from the seed coat on functional properties of pickled baby corn, as well ...
Marijana Simić +6 more
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Fabrication of quencher-free liquid scintillator-based, high-activity 222Rn calibration sources for the Borexino detector [PDF]
Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 2018 A reliable and consistently reproducible technique to fabricate $^{222}$Rn-loaded radioactive sources ($\sim$0.5-1 kBq just after fabrication) based on liquid scintillator (LS), with negligible amounts of LS quencher contaminants, was implemented. This work demonstrates the process that will be used during the Borexino detector's upcoming calibration ...
Bravo-Berguño, David +5 more
openaire +2 more sources
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source
New organic PVC photo-stabilizers derived from synthesised novel coumarine moieties
Materials Science for Energy Technologies, 2022 New four coumarin derivatives (9–12) compounds have been successfully synthesized from the reaction of salicyaldehyde and ethylacetoacetate yielding 3-acetyl coumarine, which on further treatment with bromine/chloroform gave 3-(2-Bromoacetyl) coumarine ...
Suror A. Mahdi +6 more
doaj +1 more source
FEBS Letters, EarlyView.Protein aggregates threaten proteostasis and cell health. In human cells, Hsp70–J‐domain protein‐based disaggregases remove aggregates, but how they assemble remains unclear. Our biochemical findings show that DNAJA2‐ and DNAJB1‐containing disaggregase scaffolds enhance luciferase aggregate targeting, and that Hsp70 recruitment by both J‐domain ...
Anna Szlachcic, Nadinath B. Nillegoda
wiley +1 more source
Molecular Oncology, EarlyView.Loss of the miR‐214/199a cluster is associated with recurrence in ovarian cancer. Engineered small extracellular vesicles (m214‐sEVs) elevate miR‐214‐3p/miR‐199a‐5p in tumor cells, suppress β‐catenin, TLR4, and YKT6 signaling, reprogram tumor‐derived sEV cargo, reduce chemoresistance and migration, and enhance carboplatin efficacy and survival in ...
Weida Wang +12 more
wiley +1 more source