Results 41 to 50 of about 649 (103)
Stem Cells International, Volume 2019, Issue 1, 2019., 2019 Adipose‐derived mesenchymal stem cells (MSC‐ATs) are representative cell sources for cell therapy. However, how cell stress resulting from passage influences the MSC‐AT protein expression has been unclear. In this study, a protein expression analysis was performed by liquid chromatography with tandem mass spectrometry (LC‐MS/MS) using mouse primary ...
Yoshiki Nakashima +9 more
wiley +1 more source
Efficient enzyme‐free isolation of brain‐derived extracellular vesicles
Journal of Extracellular Vesicles, Volume 13, Issue 11, November 2024.Abstract Extracellular vesicles (EVs) have gained significant attention as pathology mediators and potential diagnostic tools for neurodegenerative diseases. However, isolation of brain‐derived EVs (BDEVs) from tissue remains challenging, often involving enzymatic digestion steps that may compromise the integrity of EV proteins and overall ...
Andreu Matamoros‐Angles +16 more
wiley +1 more source
A reference genome of the Chinese hamster based on a hybrid assembly strategy
Biotechnology and Bioengineering, Volume 115, Issue 8, Page 2087-2100, August 2018., 2018 The authors completely resequenced the Chinese hamster genome using Single Molecule Real Time (SMRT) sequencing and merged the resulting SMRT‐based assembly with the publicly available draft Illumina‐based assemblies. The new assembly is more contiguous and complete than genome assemblies from either technology alone, reducing the number of scaffolds ...
Oliver Rupp +17 more
wiley +1 more source
Traffic, Volume 25, Issue 5, May 2024.The polymorphic APOE gene is the greatest genetic determinant of sporadic Alzheimer’s disease risk: the APOE4 allele increases risk, while the APOE2 allele is neuroprotective compared with the risk‐neutral APOE3 allele. In contrast to APOE4’s detrimental effects on endosomal pathways, aged APOE2 mice brains show endosomal pathway resiliance, stable ...
Katherine Y. Peng +12 more
wiley +1 more source
Rab GTPases in Osteoclastic Endomembrane Systems
BioMed Research International, Volume 2018, Issue 1, 2018., 2018 Osteoclasts (OCs) are bone‐resorbing cells that maintain bone homeostasis. OC differentiation, survival, and activity are regulated by numerous small GTPases, including those of the Rab family, which are involved in plasma membrane delivery and lysosomal and autophagic degradation pathways.
Michèle Roy, Sophie Roux, Wen-Hwa Lee
wiley +1 more source
International audienceEndocytosis and endocytic recycling allow cells to sense their environment and adapt their metabolism. T cells, which need rapid metabolic flexibility to mount effective immune responses, rely on such adaptation.
Cormont, Mireille
core +4 more sources
Detection of HIV‐1 and Human Proteins in Urinary Extracellular Vesicles from HIV+ Patients
Advances in Virology, Volume 2018, Issue 1, 2018., 2018 Background. Extracellular vesicles (EVs) are membrane bound, secreted by cells, and detected in bodily fluids, including urine, and contain proteins, RNA, and DNA. Our goal was to identify HIV and human proteins (HPs) in urinary EVs from HIV+ patients and compare them to HIV− samples. Methods. Urine samples were collected from HIV+ (n = 35) and HIV− (n
Samuel I. Anyanwu +9 more
wiley +1 more source
HEPATIC GLUCOSE PRODUCTION BY GLYCOPHAGY IS CONTROLLED BY THE SMALL GTPASE RAB4B
International audienceBackground: Endocytic trafficking, controlled by small GTPases of the Rab family, regulates the intracellular transport of metabolic signaling receptors and nutrients, either for degradation via late endosomes and lysosomes, or back
Cormont, Mireille +7 more
core +4 more sources
IST1 regulates select recycling pathways
Traffic, Volume 25, Issue 1, January 2024.We report that ESCRT‐III protein IST1 and its binding partner CHMP1B contribute to scission of endosomal carriers and that IST1 is required to efficiently deliver select cargo from early/sorting endosomes to recycling endosomes. IST1 also interacts with SNX15, and together they localize to a subdomain of early/sorting endosomes distinct from those ...
Amy K. Clippinger +5 more
wiley +1 more source
Control of mitochondrial functions by a mechanism dependent on Rab4b
International audienceBackground: Endocytosis and endocytic recycling are key processes that inform cells about their environment in order to adapt their metabolism to fulfill their role.
Cormont, Mireille +6 more
core +2 more sources