Results 11 to 20 of about 5,056 (155)

Effect of High-Pressure Processing Operating Parameters on Microbial Inactivation and Bioactive Protein Preservation in Bovine Milk: A Systematic Review. [PDF]

Compr Rev Food Sci Food Saf
ABSTRACT In the U.S., bovine milk is processed using thermal pasteurization to ensure microbial safety. However, this process alters the structure of heat‐sensitive bioactive proteins associated with the functional benefits of raw milk, including antimicrobial, immunomodulatory, and antioxidant proteins.
Sykora R, Mark C, Biondi Ryan M, Barman B, Pitino M, Dallas DC.   +5 more
europepmc   +2 more sources

A Monoclonal Antibody ELISA-Based Assay for Measuring the Potency of Candidate H5 Clade 2.3.4.4b Pandemic Influenza Vaccines. [PDF]

Influenza Other Respir Viruses
ABSTRACT Background The single radial immunodiffusion (SRID) assay is used to determine the potency of inactivated influenza vaccines. Nevertheless, development of alternative influenza vaccine potency assays with greater sensitivity and less reliance on large quantities of reference reagents is needed.
Odin M, Schmeisser F, Soto J, Weir JP.
europepmc   +2 more sources

Diagnostic accuracy of a Brix refractometer to estimate porcine colostrum quality. [PDF]

Vet Rec
Abstract Background The objective of this study was to investigate the validity of the Brix refractometer to accurately estimate immunoglobulin G (IgG) concentrations in porcine colostrum, using radial immunodiffusion (RID) as a reference standard. Methods Eighty‐seven composite colostrum samples were collected from sows on a single pig farm between ...
O'Brien ÁC, Farish M, Baxter EM, Denholm KS.   +3 more
europepmc   +2 more sources

Determination of Fibrinogen Ratio Cutoff Limits Using Indirect Reference Interval Methodology. [PDF]

Int J Lab Hematol
ABSTRACT Introduction Discordant fibrinogen antigen to activity ratios are utilized by clinicians as evidence of dysfibrinogenemia. Abnormal ratio cutoffs implemented by clinical laboratories are typically determined by validation studies that include limited numbers of samples.
Saadalla A, Doyle K, Moser K, Smock K.
europepmc   +2 more sources

Developing tools for learning immunology using diffusion-based salt precipitation assays: A low-cost alternative for college laboratories. [PDF]

Biochem Mol Biol Educ
Abstract The Ouchterlony double immunodiffusion technique is used as a teaching tool for studying immune responses and exemplifying differences in antigen–antibody reactions. Although commonplace in undergraduate labs, standardized commercial kits limit learning experiences because they have fixed modalities of use, a low shelf‐life, and impose ...
Pal A, Sen S.
europepmc   +2 more sources

Evaluation of Immunogenicity of Seasonal Influenza Split HA Vaccines Involved With Changes of Vaccine Strains Using Mouse and Human Sera. [PDF]

Microbiol Immunol
ABSTRACT Seasonal influenza is prevalent every winter, and influenza vaccines are used to safeguard public health. As the influenza vaccines are produced in embryonated hen eggs using vaccine viruses recommended by the World Health Organization each year based on the epidemic situation, it is important, from the perspective of public health, to ...
Sato K, Shimasaki N, Fujisaki S, Nishijima H, Kuwahara T, Nakai Y, Murano K, Asanuma H, Watanabe S, Harada Y, Ryo A, Tashiro M, Itamura S.   +12 more
europepmc   +2 more sources

Determination of the Reliability and Accuracy of the Brix Refractometer for the Detection of Colostrum Quality in Sheep. [PDF]

Vet Med Sci
ABSTRACT This study was aimed at the assessment of the reliability and performance of the Brix refractometer for the determination of colostral IgG concentrations in sheep. A total of 190 colostrum samples were collected from 150 ewes of various breeds.
Akköse M, Özbeyaz C, Buczinski S, Gündoğar U, Ünal N.   +4 more
europepmc   +2 more sources

Method Comparison of Dried Blood Spot and Plasma Apolipoprotein B Measurement on a Semi-Automated Analyser: Toward Accessible Lipid Profiling in Resource-Limited Settings. [PDF]

Anal Sci Adv
ABSTRACT Apolipoprotein B (ApoB) is a key marker of atherogenic lipoprotein burden, but conventional plasma‐based testing requires venous sampling and centralized laboratory infrastructure. Dried blood spot (DBS) sampling offers a minimally invasive alternative suitable for decentralized settings.
Pitampersad B, Madurai S, Manivannan B, Pillay K, Mahomed S.   +4 more
europepmc   +2 more sources

Determining Immunoglobulin Content of Bovine Colostrum and Factors Affecting the Outcome: A Review

Animals, 2021
The immunoglobulin concentration in bovine colostrum should be measured to ensure feeding with sufficient immunoglobulins (≥50 mg immunoglobulin G mL−1).
Johanna Ahmann, Julia Steinhoff-Wagner, Wolfgang Büscher   +2 more
doaj   +1 more source

Silver-enhanced radial immunodiffusion assay of plasma apolipoproteins.

Journal of Lipid Research, 1992
Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-I, A-II, C, and E at levels of 0.2-1.0 mg/dl in plasma samples at a ...
BY Ishida, B Paigen
doaj   +1 more source