Results 51 to 60 of about 4,434,687 (184)

Similarity‐Enhanced Representation Learning of Non‐Canonical Amino Acids for Therapeutic Peptide Modeling

Advanced Science, EarlyView.
Non‐canonical amino acids (ncAAs) enhance peptide therapeutics but remain difficult to model computationally. SinCAA, a similarity‐enhanced pretraining framework, jointly optimizes contrastive learning guided by a novel conformational similarity metric with masked node reconstruction, capturing both functional relationships and chemical identity of ...
Chencheng Xu, Lesong Wei, Jianmin Wang, Yuanpeng Xiong, Ruochi Zhang, Yu Wang, Chao Zha, Qiangcheng Zeng, Xin Gao   +8 more
wiley   +1 more source

Kap-Centric control of nuclear pores based on promiscuous binding to FG nucleoporins [PDF]

, 2014
Nuclear pore complexes (NPCs) are remarkable molecular machines that perforate the nuclear envelope (NE) in eukaryotic cells and mediate the rapid bidirectional traffic of hundreds of proteins, ribonucleoproteins, and metabolites across the nuclear ...
Wagner, Raphael S.
core   +1 more source

Astrocytes Coordinate Neurovascular Coupling During Prolonged Sensory Demand via AQP4/TRPA1/BEST1/D‐Serine Signaling

Advanced Science, EarlyView.
Neuronal activation leads to astrocytic transient volume changes, activating transient receptor potential ankyrin 1 (TRPA1) channels, leading to Ca2+ influx. Activation of TRPA1 causes the release of D‐serine through BEST1. Finally, the augmented extracellular levels of D‐serine boost neuronal activity by binding GluN1‐containing NMDARs, leading to CBV
Kayoung Han, Minwoo Wendy Jang, Taeyoung Park, Ki Jung Kim, Victor James Drew, Sunpil Kim, Tai Young Kim, Mingu Gordon Park, Myeongju Kim, Seung Won Chung, Minah Suh, C. Justin Lee   +11 more
wiley   +1 more source

An HflX-type GTPase from Sulfolobus solfataricus binds to the 50S ribosomal subunit in all nucleotide-bound states [PDF]

, 2011
HflX GTPases are found in all three domains of life, Bacteria, Archaea, and Eukaryotes. HflX from Escherichia coli has been shown to bind to the 50S ribosomal subunit in a nucleotide-dependent manner and this interaction strongly stimulates its GTPase ...
Fabian Blombach, J. Van Der Oost, Daan C. Swarts, John van der Oost, Londei, P., LONDEI, Paola, Christodoulou, John, John Christodoulou, Dario Benelli, D. c. Swarts, Lisa D. Cabrita, Launay, H., BENELLI, DARIO, H. Launay, Benelli, D., L. d. Cabrita, Violeta Zorraquino, Zorraquino, V., Cabrita, L.D., V. Zorraquino, Oost, J., van der, F. Blombach, J. Christodoulou, Helene Launay, Paola Londei, van der Oost, J., Blombach, F., Swarts, D.C.   +27 more
core   +1 more source

Identification and functional characterization of the novel MAR-binding protein, SATB2 [PDF]

, 2004
The regulation of gene expression is governed in large part by transcription factors that bind to enhancers and promoters. The functions of transcription factors involve both the modulation of chromatin accessibility via the recruitment of histone ...
Dobreva, Gergana
core   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Structure and function of bacterial dynamin-like proteins [PDF]

, 2012
Membrane dynamics are essential for numerous cellular processes in eukaryotic and prokaryotic cells. In eukaryotic cells, membrane fusion and fission are often catalyzed by large GTPases of the dynamin protein family. These proteins couple GTP hydrolysis
Bramkamp, Marc
core   +1 more source

Identification of Putative Cellulose Synthase Gene xcsA2 Required for c‐di‐GMP‐Induced Biofilm Formation and Cell Aggregation in Synechococcus elongatus PCC 7942

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Synechococcus elongatus PCC 7942 is a widely used model cyanobacterium for diverse research fields and for bioproduction applications owing to its ease of cultivation, genetic modification, and ability to fix CO2. We found that a laboratory strain of S. elongatus PCC 7942 expressing the ydeH gene from E.
Chihiro C. Yamaguchi, Jiro F. Mori, Shinsuke Kutsuna, Robert A. Kanaly   +3 more
wiley   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source