Results 51 to 60 of about 29,147 (185)

Gz, a guanine nucleotide-binding protein with unique biochemical properties [PDF]

, 1990
Cloning of a complementary DNA (cDNA) for Gz alpha, a newly appreciated member of the family of guanine nucleotide-binding regulatory proteins (G proteins), has allowed preparation of specific antisera to identify the protein in tissues and to assay it ...
Casey, Patrick J., Fong, Henry K. W., Gilman, Alfred G., Simon, Melvin I.   +3 more
core  

Sensing and Filtering Environmental Fluctuations: The Case of Biomolecular Condensates in Plants

Advanced Science, EarlyView.
The diversity of plant condensates reflects constraints of sessile organisms to coordinate postembryonic development with environmental adaptation. This review examines how plants employ condensates to integrate temperature, light, redox, and nutrient signals.
Panagiotis N. Moschou, Dorothee Staiger
wiley   +1 more source

DMS‐MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity

Advanced Science, EarlyView.
RNA structural profiling of Turnip Yellow Mosaic Virus by DMS‐MaPseq and DREEM analyses uncover that viral genome‐wide RSS is highly complicated and heterogeneous, with alternative RSSs widely distributed across the genome. Notably, the viral 3’ tRNA‐like structure adopts alternative conformations in vivo.
Jiaying Zhu, Changhao Li, Chisimbily Onyia, Xindi Li, Xingxing Yan, Songxiao Zhong, Taerin Oh, Xiuren Zhang   +7 more
wiley   +1 more source

Karyopherins regulate nuclear pore complex barrier and transport function [PDF]

, 2017
Nucleocytoplasmic transport is sustained by karyopherins (Kaps) and a Ran guanosine triphosphate (RanGTP) gradient that imports nuclear localization signal (NLS)–specific cargoes (NLS-cargoes) into the nucleus.
Adam, Allen, Bayliss, Bednenko, Ben-Efraim, Binlu Huang, Bischoff, Catimel, Chaillan-Huntington, Chantal Rencurel, Chook, Christie, Cingolani, Cingolani, Conti, Delphin, Eibauer, Eisele, Falces, Floer, Forwood, Forwood, Frey, Fukuhara, Grünwald, Görlich, Görlich, Görlich, Görlich, Hahn, Halder, Hough, Isgro, Kapinos, Kau, Kimura, Kopito, Kubitscheck, Kutay, Kutay, Larisa E. Kapinos, Lee, Lee, Lim, Lim, Lolodi, Lonhienne, Lowe, Lyman, Makise, Milles, Moroianu, Moroianu, Nigg, Ogawa, Paradise, Popken, Pumroy, Pyhtila, Rexach, Ribbeck, Riddick, Roderick Y.H. Lim, Rout, Sakiyama, Schoch, Stewart, Strawn, Sun, Tauchert, Timney, Tokunaga, Tu, von Appen, Vovk, Wagner, Weis, Yamada, Yang, Yang, Yoshimura, Zachariae   +81 more
core   +1 more source

The Role of miRNAs in Chicken Immune Regulation and Prospects for Disease‐Resistant Breeding

Animal Research and One Health, EarlyView.
A schematic workflow illustrating the screening of disease‐resistant miRNAs and the generation of miRNA‐based disease‐resistant chickens via PGC‐mediated germline genome editing. ABSTRACT MicroRNAs (miRNAs) are emerging as pivotal regulators of the immune system, playing a decisive role in shaping disease resistance in chicken.
Qiangzhou Wang, Weiran Wu, Qingqing Cai, Jiaxing Wang, Qi Gao, Muhammad Iftikhar, Yulin Bi, Hao Bai, Lihong Gu, Guobin Chang, Shihao Chen   +10 more
wiley   +1 more source

Importin-β modulates the permeability of the nuclear pore complex in a Ran-dependent manner [PDF]

, 2015
Soluble karyopherins of the importin-β (impβ) family use RanGTP to transport cargos directionally through the nuclear pore complex (NPC). Whether impβ or RanGTP regulate the permeability of the NPC itself has been unknown. Here, we identify a stable pool
Adam, Andersen, Askjaer, Ball, Bischoff, Bischoff, Cheezum, Chen, Chook, Chung, Conover, Forstner, Frenkiel-Krispin, Frey, Gorlich, Gorlich, Harborth, Heilemann, Hitakomate, Hood, Izaurralde, Jovanovic-Talisman, Kalab, Kapinos, Leake, Li, Li, Lim, Lim, Loschberger, Lowe, Lyman, Ma, Milles, Moore, Moore, Muller, Newberry, Nitsche, Peters, Peters, Pollard, Rabut, Ribbeck, Ribbeck, Rout, Saitoh, Sampathkumar, Schleicher, Schmidt, Schrader, Shah, Shah, Snow, Stewart, Stuwe, Sun, Tetin, Thompson, Tokunaga, van de Linde, Walther, Xu, Yoon, Zhou, Zilman, Zilman   +66 more
core   +1 more source

Optimizing Pichia Pastoris Cell‐Free Protein Synthesis to Improve Economics

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Cell‐free protein synthesis (CFPS) is a powerful and versatile platform that supports a wide range of applications, from fundamental studies of the genetic code to scalable and rapid protein production. The recently developed Pichia pastoris CFPS combines advantages of both prokaryotic and eukaryotic systems, including a rapid growth rate ...
Rui Wu, Igor Koop, Gavin Murugan, Thagshanen Thillairajah, Karen M. Polizzi   +4 more
wiley   +1 more source

A Modular Mechanistic In Silico Model for In Vitro Transcription Process Yield and Product Quality Prediction

Biotechnology and Bioengineering, EarlyView.
ABSTRACT In vitro transcription (IVT) plays a critical role in the manufacture of mRNA vaccines and therapeutics. Optimizing mRNA yield and ensuring product quality, such as capping efficiency and integrity, are essential but mechanistically complex. This study presents a modular mechanistic model of the IVT process to advance scientific understanding ...
Keqi Wang, Keilung Choy, Eli Reiser, Jinxiang Pei, Hua Zheng, Aparajita Dasgupta, Fuqiang Cheng, Guogang Dong, Bhanu Chandra Mulukutla, Joshua Mannheimer, Carolyn Huang, Hooman Farsani, Wei Xie   +12 more
wiley   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Change in Nutritional Status Modulates the Abundance of Critical Pre-initiation Intermediate Complexes During Translation Initiation \u3cem\u3ein Vivo\u3c/em\u3e [PDF]

, 2007
In eukaryotic translation initiation, eIF2∙GTP–Met-tRNAiMet ternary complex (TC) interacts with eIF3–eIF1–eIF5 complex to form the multifactor complex (MFC), while eIF2∙GDP associates with eIF2B for guanine nucleotide exchange.
Anderson, James T., Asano, Katsura, He, Hei, Lee, Bumjun, Pavitt, Graham D., Singh, Chingakham Ranjit, Udagawa, Tsuyoshi, Wassink, Sarah, Yamamoto, Yasufumi   +8 more
core   +1 more source