Results 91 to 100 of about 837,711 (307)
Lego DNA assembling, a simple in vitro method for constructing DNA molecule
, 2010 Digestion-ligation based and recombination based methods for constructing recombinant DNA are the basic techniques in molecular biology and thus built one of the foundations for the modern life sciences. Here we describe a new strategy that can radically
Cong Wang +7 more
core
Molecular cloning and physical mapping of murine cytomegalovirus DNA [PDF]
, 1983 Murine cytomegalovirus (MCMV) Smith strain DNA is cleaved by restriction endonuclease HindIII into 16 fragments, ranging in size from 0.64 to 22.25 megadaltons.
Ebeling, Angelika +3 more
core
Automatic Preparation of DNA Templates for Sequencing on the ABI Catalyst Robotic Workstation
, 1994 The need for automation of the various steps in DNA sequencing increases as the number of applications and genomic sequencing applications grow. It is therefore necessary to design robotic workstations for template preparation and sequencing reactions in
Holmberg, A., +5 more
core +1 more source
Metal complexes as DNA intercalators [PDF]
, 2011 DNA has a strong affinity for many heterocyclic aromatic dyes, such as acridine and its derivatives. Lerman in 1961 first proposed intercalation as the source of this affinity, and this mode of DNA binding has since attracted considerable research ...
Peter J. Sadler +3 more
core +1 more source
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
Rapid analysis technologies with chemometrics for food authenticity field: A review
Current Research in Food ScienceIn recent years, the problem of food adulteration has become increasingly rampant, seriously hindering the development of food production, consumption, and management.
Zixuan Zhang +7 more
doaj +1 more source
Design and analysis strategies for robust microbiome ageing research
FEBS Letters, EarlyView.The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik +5 more
wiley +1 more source
Reproducible genomic DNA preparation from diverse crop species for molecular genetic applications
Plant Methods, 2017 Background Several high-throughput molecular genetic analyses rely on high-quality genomic DNA. Copurification of other molecules can negatively impact the functionality of plant DNA preparations employed in these procedures.
Kelvin T. Chiong +8 more
doaj +1 more source
FEBS Letters, EarlyView.CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens +8 more
wiley +1 more source
Frontiers in Genetics, 2018 Background:FMR1 CGG trinucleotide repeat hyper-expansions are observed in 99% of individuals with fragile X mental retardation syndrome (FXS). We evaluated the reliability of a rapid single-step gender-neutral molecular screen for FXS when performed on ...
Vivienne J. Tan +6 more
doaj +1 more source