Results 101 to 110 of about 837,711 (307)

A Dynamic Stochastic Model for DNA Replication Initiation in Early Embryos

, 2008
Background: Eukaryotic cells seem unable to monitor replication completion during normal S phase, yet must ensure a reliable replication completion time.
Labit, H, Goldar, A, Marheineke, K, Olivier Hyrien, Hélène Labit, Hyrien, O, Kathrin Marheineke, Arach Goldar   +7 more
core   +1 more source

Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction [PDF]

, 1992
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid
Dunham, Ian, Cremer, Thomas, Terry Featherstone, Christoph Lengauer, Featherstone, Terry, Lan Dunham, Lengauer, Christoph, Thomas Cremer   +7 more
core   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Development of Lasing Silica Microsphere for High-Speed DNA Molecular Detection

Sensors
Laser and molecular detection techniques that have been used to overcome the limitations of fluorescent DNA labeling have presented new challenges. To address some of these challenges, we developed a DNA laser that uses a solid-state silica microsphere ...
Chan Seok Jun, Wonsuk Lee
doaj   +1 more source

Expression in mammalian cells of a cloned gene encoding murine DNA methyltransferase [PDF]

, 1991
Mammalian DNA cytosine-5-methyltransferase (MTase, EC 2.1.1.37) is an essential component for establishing and maintaining cell-type specific methylation patterns in the genome. The cDNAfor the murine enzyme was previously cloned in segments.
Häuselmann, Remo, Bestor, Timothy H., Bestor, T. H., Page, A. W., Page, Andrea W., Hergersberg, M., Leonhardt, Heinrich, Häuselmann, R., Czank, A., Czank, Andreas, Schaffner, Walter, Schaffner, W., Hergersberg, Martin, Leonhardt, H.   +13 more
core   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Circular RNA expression landscapes in myelodysplastic neoplasms: Associations with mutational signatures and disease progression

Molecular Oncology, EarlyView.
In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge, Morten Tulstrup, Gabriele Todisco, Pedro Moura, Christophe Côme, Jakob Werner Hansen, Jakob Schmidt Jespersen, Balthasar Schlotmann, Maria Creignou, Mette Dahl, Claudia Schöllkopf, Klas Raaschou‐Jensen, Krister Wennerberg, Bo Porse, Joachim Weischenfeldt, Eva Hellström‐Lindberg, Lasse Sommer Kristensen, Kirsten Grønbæk   +17 more
wiley   +1 more source

A Simple Method for Sexing Chicken Embryos at Stage X

The Journal of Poultry Science, 2003
A simple method for sexing chicken embryos was devised using DNA prepared from blastodermal cells. A cluster of cells was collected from the area opaca of a stage X blastoderm, and DNA was prepared by the boiling method (10-minute boiling and 3-minute ...
Mitsuru Naito, Akiko Sano, Takaharu Kawashima, Hiroyuki Nakamichi, Takashi Harumi, Yuko Matsubara, Takashi Kuwana   +6 more
doaj   +1 more source

Evolution of repetitive DNA in angiosperms: examples from Nicotiana allopolyploids

, 2012
PhDAllopolyploidy, interspecific hybridisation coupled with genome multiplication, is a prevailing force in the evolution of angiosperms. This thesis examines the consequences of allopolyploidy at the genomic level.
Renny-Byfield, Simon
core  

Low-cost rapid detection of rifampicin resistant tuberculosis using bacteriophage in Kampala, Uganda. [PDF]

, 2007
BACKGROUND: Resistance to anti-tuberculosis drugs is a serious public health problem. Multi-drug resistant tuberculosis (MDR-TB), defined as resistance to at least rifampicin and isoniazid, has been reported in all regions of the world.
Traore, Hamidou, Hamidou Traore, Jones-Lopez, Edward C, McNerney, Ruth, Eisenach Kathleen D, Joloba, Moses L, Jones-Lopez Edward C, Roy D Mugerwa, Smith, Peter G, Mugerwa, Roy D, Ogwang Sam, Smith Peter G, Moses L Joloba, Mallard, Kim, Kathleen D Eisenach, Jerrold J Ellner, Ellner, Jerrold J, Eisenach, Kathleen D, Ogwang, Sam, Mugerwa Roy D, Kayes, Susan, McNerney Ruth, Edward C Jones-Lopez, Kalpana Narayan, Ruth McNerney, Peter G Smith, Sam Ogwang, Susan Kayes, Joloba Moses L, Narayan, Kalpana, Francis Mumbowa, Narayan Kalpana, Ellner Jerrold J, Mumbowa, Francis, Kayes Susan, Kim Mallard, Traore Hamidou, Mumbowa Francis, Mallard Kim   +38 more
core   +1 more source