Results 111 to 120 of about 38,509 (306)

Polymer Tools Produced by Fused Filament Fabrication for Steel-Bending Process: Effect of Layering Orientation

open access: yesJournal of Manufacturing and Materials Processing
Rapid tooling with polymer tools produced via additive manufacturing offers significant benefits in sheet metal forming processes as it allows for the production of parts with high accuracy while reducing tool production costs.
Luca Giorleo, Kudret Irem Deniz
doaj   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

The potential of additive manufacturing in the smart factory industrial 4.0: A review [PDF]

open access: yes, 2019
Additive manufacturing (AM) or three-dimensional (3D) printing has introduced a novel production method in design, manufacturing, and distribution to end-users.
Dehghanghadikolaei, Amir   +5 more
core   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

open access: yesFEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold   +11 more
wiley   +1 more source

Fabrication of Graphene Nanoparticle Reinforced Feedstock Filaments for Sustainable Rapid Tooling and Green Manufacturing

open access: yesJournal of Nanotechnology
The growing demand for sustainable rapid tooling in 3D printing highlights the requirement for such advanced materials that are designed using the framework of green chemistry principles.
Suraj Ghising   +5 more
doaj   +1 more source

Computer simulation of thermoforming process and its verification using a rapid tooling mould

open access: yesMATEC Web of Conferences, 2018
The results of computer simulation of thermoforming process made using ANSYS Polyflow software are presented in this paper. The analysis of the wall thickness distribution across an U-shaped thermoformed product manufactured using a positive mould was ...
Modławski Michał, Jaruga Tomasz
doaj   +1 more source

Design for Additive Manufacturing of Conformal Cooling Channels Using Thermal-Fluid Topology Optimization and Application in Injection Molds [PDF]

open access: yes, 2018
Additive manufacturing allows the fabrication parts and tools of high complexity. This capability challenges traditional guidelines in the design of conformal cooling systems in heat exchangers, injection molds, and other parts and tools.
Tovar, Andres, Wu, Tong
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

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