Results 1 to 10 of about 399,396 (198)

Reducing the effects of PCR amplification and sequencing artifacts on 16S rRNA-based studies. [PDF]

open access: yesPLoS ONE, 2011
The advent of next generation sequencing has coincided with a growth in interest in using these approaches to better understand the role of the structure and function of the microbial communities in human, animal, and environmental health.
Patrick D Schloss   +2 more
doaj   +11 more sources

FASTQuick: rapid and comprehensive quality assessment of raw sequence reads. [PDF]

open access: yesGigascience, 2021
Abstract Background Rapid and thorough quality assessment of sequenced genomes on an ultra-high-throughput scale is crucial for successful large-scale genomic studies. Comprehensive quality assessment typically requires full genome alignment, which costs a substantial amount of computational ...
Zhang F, Kang HM.
europepmc   +5 more sources

Scalable long read self-correction and assembly polishing with multiple sequence alignment

open access: yesScientific Reports, 2021
Third-generation sequencing technologies allow to sequence long reads of tens of kbp, that are expected to solve various problems. However, they display high error rates, currently capped around 10%.
Pierre Morisse   +4 more
doaj   +1 more source

De novo characterization of the Lycium ruthenicum transcriptome and analysis of its digital gene expression profiles during fruit development and ripening [PDF]

open access: yesArchives of Biological Sciences, 2017
Lycium ruthenicum Murr., which belongs to the family Solanaceae, is a resource plant for Chinese traditional medicine and nutraceutical foods. In this study, RNA sequencing was applied to obtain raw reads of L.
Peng Yong, Ma Huiqin, Chen Shangwu
doaj   +1 more source

RNA-seq data from C-X-C chemokine receptor type 5 (CXCR5) gene knockout aged mice with retinal degeneration phenotype

open access: yesData in Brief, 2020
The CXCR5 (C-X-C motif chemokine receptor 5) is chemokine transmembrane receptor, acting via its ligand CXCL13 and plays a crucial role in controlling the trafficking of inflammatory cells into and from the sub-retinal space, which contributes to the ...
Madhu Sudhana Saddala   +2 more
doaj   +1 more source

Rapid multi-locus sequence typing direct from uncorrected long reads using Krocus [PDF]

open access: yesPeerJ, 2018
Genome sequencing is rapidly being adopted in reference labs and hospitals for bacterial outbreak investigation and diagnostics where time is critical.
Andrew J. Page, Jacqueline A. Keane
doaj   +2 more sources

The complete genome sequence of Stevia rebaudiana, the Sweetleaf [version 1; peer review: 2 approved, 1 approved with reservations]

open access: yesF1000Research, 2020
The Sweetleaf (Stevia rebaudiana: Asteraceae) is widely grown for use as a sweetener.  We present the whole genome sequence and annotation of this species.  A total of 146,838,888 paired-end reads consisting of 22.2G bases were obtained by sequencing one
Kathleen O'Neill, Stacy Pirro
doaj   +1 more source

Detection of microRNAs in color space [PDF]

open access: yes, 2011
MotivationDeep sequencing provides inexpensive opportunities to characterize the transcriptional diversity of known genomes. The AB SOLiD technology generates millions of short sequencing reads in color-space; that is, the raw data is a sequence of ...
Altschul   +29 more
core   +2 more sources

RNA-seq data from different developmental stages of Rafflesia cantleyi floral buds

open access: yesGenomics Data, 2017
Rafflesia cantleyi, known as one of the world's largest flowers, is a specialised holoparasite due to dramatic morphological modifications. It possesses highly reduced vegetative structure and only appears as a flower for sexual reproduction.
Safoora Amini   +6 more
doaj   +1 more source

The complete genome sequence of Toxicodendron radicans, Eastern Poison Ivy [version 1; peer review: 2 approved]

open access: yesF1000Research, 2020
Eastern Poison Ivy (Toxicodendron radicans, Anacardiaceae) is well known in Eastern North America for causing contact dermatitis, an itchy and painful rash in most people who come in contact with it.
Toby Pirro, Stacy Pirro
doaj   +1 more source

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