Results 11 to 20 of about 34,349 (240)

Resource-efficient internally controlled in-house real-time PCR detection of SARS-CoV-2

open access: yesVirology Journal, 2021
Background The reliable detection of SARS-CoV-2 has become one of the most important contributions to COVID-19 crisis management. With the publication of the first sequences of SARS-CoV-2, several diagnostic PCR assays have been developed and published ...
Janine Michel   +16 more
doaj   +1 more source

Real-Time Polymerase Chain Reaction: Applications in Diagnostic Microbiology

open access: yesInternational Journal of Medical Students, 2013
The polymerase chain reaction (PCR) has revolutionized the detection of DNA and RNA. Real-Time PCR (RT-PCR) is becoming the gold standard test for accurate, sensitive and fast diagnosis for a large range of infectious agents.
Kordo B.A. Saeed, Nusreen S. Ahmad
doaj   +1 more source

Development and validation of an in-house, low-cost SARS-CoV-2 detection assay

open access: yesJournal of Infection and Public Health, 2021
Background: One major challenge for detecting the virus that causes COVID-19 is commercial SARS-CoV-2 testing kit or reagent availability. To allow every laboratory or hospital access to an in-house assay, we developed a low-cost SARS-CoV-2 detection ...
Fatimah S. Alhamlan   +7 more
doaj   +1 more source

Effects of upconversion nanoparticles on polymerase chain reaction. [PDF]

open access: yesPLoS ONE, 2013
Nanoparticles (NPs) are attractive materials owing to their physical and electrochemical properties, which make them extremely useful in diagnostic applications.
Sang-Hyun Hwang   +8 more
doaj   +1 more source

Evaluation of serological test of Zika in an endemic area of flavivirus in the Colombian Caribbean

open access: yesAnnals of Clinical Microbiology and Antimicrobials, 2019
The Zika virus (ZIKV) is an emerging flavivirus transmitted primarily through arthropods, endemic in Africa, Asia, and the Americas, and is considered a global threat by the World Health Organization.
German Arrieta   +4 more
doaj   +1 more source

Molecular methods for diagnosing novel coronavirus infection: comparison of loop-mediated isothermal amplification and polymerase chain reaction

open access: yesВопросы вирусологии, 2022
Introduction. Currently, the basis for molecular diagnostics of most infections is the use of reverse transcription polymerase chain reaction (RT-PCR). Technologies based on reverse transcription isothermal loop amplification (RT-LAMP) can be used as an ...
V. G. Akimkin   +9 more
doaj   +1 more source

Development and evaluation of a differential diagnostic test strip for antibodies induced by HIV-1 vaccine and natural infections

open access: yesDi-san junyi daxue xuebao, 2019
Objective To develop a differential diagnostic kit for distinguishing antibodies induced by HIV-1 infection and HIV-1 vaccine and evaluate its diagnostic performance.
YUAN Zuowei   +3 more
doaj   +1 more source

Risk Management in the Manufacturing and Use of the Medical Product for <i>in vitro</i> Diagnostics: Reagent Kit “Lyophilized Erythrocyte Antigenic Tularemia Diagnosticum”

open access: yesProblems of Particularly Dangerous Infections, 2022
The aim of the work was to implement the risk management strategies in the manufacturing and use of medical products for in vitro diagnostics by the example of the experimental series of the reagent kit “Lyophilized erythrocyte antigenic tularemia diagnosticum”.Materials and methods.
O. L. Startseva   +5 more
openaire   +2 more sources

Effective Approaches to Study the Genetic Variability of SARS-CoV-2

open access: yesViruses, 2022
Significant efforts are being made in many countries around the world to respond to the COVID-19 pandemic by developing diagnostic reagent kits, identifying infected people, determining treatment methods, and finally producing effective vaccines. However,
Ivan Kotov   +7 more
doaj   +1 more source

Diagnostic performance evaluation of hepatitis B e antigen rapid diagnostic tests in Malawi

open access: yesBMC Infectious Diseases, 2021
Background The World Health Organization (WHO) has targeted a reduction in viral hepatitis-related mortality by 65% and incidence by 90% by 2030, necessitating enhanced hepatitis B treatment and prevention programmes in low- and middle-income countries ...
Alexander J. Stockdale   +5 more
doaj   +1 more source

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